Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JWZ98_RS21455 Genome accession   NZ_CP070494
Coordinates   4676214..4677347 (-) Length   377 a.a.
NCBI ID   WP_171696916.1    Uniprot ID   -
Organism   Methylomonas sp. EFPC1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4671214..4682347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWZ98_RS21435 (JWZ98_21435) - 4671931..4672827 (+) 897 WP_205451821.1 MBL fold metallo-hydrolase -
  JWZ98_RS21440 (JWZ98_21440) - 4673303..4673839 (+) 537 WP_205451823.1 transposase -
  JWZ98_RS21445 (JWZ98_21445) - 4673960..4674514 (+) 555 WP_129871319.1 hypothetical protein -
  JWZ98_RS21450 (JWZ98_21450) mltB 4675015..4676196 (-) 1182 WP_205451825.1 lytic murein transglycosylase B -
  JWZ98_RS21455 (JWZ98_21455) pilU 4676214..4677347 (-) 1134 WP_171696916.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JWZ98_RS21460 (JWZ98_21460) pilT 4677362..4678399 (-) 1038 WP_101053853.1 type IV pilus twitching motility protein PilT Machinery gene
  JWZ98_RS21465 (JWZ98_21465) - 4678516..4678857 (-) 342 WP_033158299.1 TraR/DksA family transcriptional regulator -
  JWZ98_RS21470 (JWZ98_21470) - 4679003..4679908 (+) 906 WP_205451826.1 AAA family ATPase -
  JWZ98_RS21475 (JWZ98_21475) - 4679905..4680855 (+) 951 WP_205451828.1 DUF58 domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42074.41 Da        Isoelectric Point: 6.6669

>NTDB_id=540210 JWZ98_RS21455 WP_171696916.1 4676214..4677347(-) (pilU) [Methylomonas sp. EFPC1]
MDFKALLALMVEKKASDLFITAGRPPSMKVNGKVVEVSKTVLTSEQTMMLVLSIMSQRQRDEFENTHECQFALSAHSLGR
FRVSAFTQRDSAGMVLRRIETTIPSAEELHLPPVLKELIMYKRGLVMFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFIHPHKGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRSRETMQHAITFAETGHLCVCTLHANNANQAIDRI
LHFFPEEMHSQLFMDLSLNLRGIVAQQLIQRADGKGRYPAIEILLNTPLASDMIRKGEVHKLKELMKSSREHGMQTFDQA
LYDLYTAGKIGYDDALHAADSRNEVRLMIKLGAENVNFETAGMTLAENDDEGGSLFK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=540210 JWZ98_RS21455 WP_171696916.1 4676214..4677347(-) (pilU) [Methylomonas sp. EFPC1]
ATGGACTTTAAAGCCCTATTGGCCCTGATGGTGGAAAAGAAAGCGTCCGACTTGTTTATCACTGCCGGCCGGCCGCCGTC
GATGAAAGTCAACGGCAAGGTCGTCGAAGTGTCCAAAACCGTTTTAACCAGCGAACAGACCATGATGCTGGTGCTCAGCA
TTATGTCGCAGCGCCAACGCGACGAGTTTGAAAACACTCACGAATGCCAGTTTGCCTTAAGCGCCCACAGTTTGGGCCGG
TTCCGGGTCAGCGCCTTCACCCAACGCGACTCGGCCGGCATGGTGTTACGCCGCATCGAAACCACCATCCCCAGCGCCGA
GGAATTGCACCTGCCGCCGGTGTTGAAAGAACTGATCATGTACAAGCGCGGTCTGGTGATGTTTGTCGGTGCCACCGGTA
CCGGTAAATCCACCTCGCTGGCGGCCTTGATTCGGCACCGCAACGAAAACAGTAGCGGCCACATCATTTCCATTGAAGAC
CCTATCGAGTTTATCCATCCGCACAAGGGCTGCATCATTACCCAGCGCGAAGTGGGCCTGGATACCGAATCGTTCGAAGT
GGCTTTAAAAAACACCCTGCGGCAAGCGCCGGATGTGATTCTGATCGGTGAGGTCAGGAGCCGGGAAACCATGCAACACG
CCATCACCTTCGCGGAAACCGGCCACCTGTGCGTTTGCACCTTGCACGCTAATAACGCCAACCAGGCCATCGACCGCATC
CTGCATTTTTTTCCGGAAGAAATGCACAGCCAGCTGTTCATGGATTTGTCTTTGAACTTGCGCGGTATCGTCGCCCAACA
ATTGATTCAGCGCGCCGACGGCAAGGGCCGCTACCCGGCTATTGAGATTTTGCTGAATACCCCGCTGGCATCAGACATGA
TCCGCAAGGGCGAAGTACACAAATTAAAAGAACTGATGAAAAGCTCCCGCGAGCATGGCATGCAGACTTTCGACCAAGCG
CTTTACGATCTGTATACGGCGGGTAAAATTGGTTACGATGACGCCTTGCACGCTGCCGACTCCCGCAACGAGGTGCGCCT
GATGATTAAACTGGGGGCCGAGAACGTCAACTTCGAAACCGCCGGCATGACCCTGGCCGAAAACGACGACGAGGGAGGCA
GTCTTTTTAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.192

100

0.679
  pilU Acinetobacter baylyi ADP1

65.07

94.164

0.613
  pilU Vibrio cholerae strain A1552

57.143

92.838

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.253

92.308

0.408
  pilT Legionella pneumophila strain Lp02

42.478

89.92

0.382
  pilT Legionella pneumophila strain ERS1305867

42.478

89.92

0.382
  pilT Acinetobacter nosocomialis M2

40.708

89.92

0.366
  pilT Acinetobacter baumannii D1279779

40.708

89.92

0.366
  pilT Acinetobacter baumannii strain A118

40.708

89.92

0.366
  pilT Acinetobacter baylyi ADP1

40.176

90.451

0.363
  pilT Pseudomonas aeruginosa PAK

40.413

89.92

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.212

87.533

0.361
  pilT Vibrio cholerae strain A1552

41.212

87.533

0.361