Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JWZ97_RS13020 Genome accession   NZ_CP070491
Coordinates   3045078..3046112 (-) Length   344 a.a.
NCBI ID   WP_205429921.1    Uniprot ID   A0A974VQ64
Organism   Methylococcus sp. EFPC2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3040078..3051112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWZ97_RS12990 (JWZ97_12990) - 3041038..3041781 (-) 744 WP_205429902.1 S-adenosyl-l-methionine hydroxide adenosyltransferase family protein -
  JWZ97_RS12995 (JWZ97_12995) tatC 3041800..3042609 (-) 810 WP_205429912.1 twin-arginine translocase subunit TatC -
  JWZ97_RS13000 (JWZ97_13000) tatB 3042596..3042892 (-) 297 WP_205429914.1 Sec-independent protein translocase protein TatB -
  JWZ97_RS13005 (JWZ97_13005) tatA 3042907..3043110 (-) 204 WP_205429916.1 twin-arginine translocase TatA/TatE family subunit -
  JWZ97_RS13010 (JWZ97_13010) - 3043223..3043789 (-) 567 WP_205429918.1 DUF2726 domain-containing protein -
  JWZ97_RS13015 (JWZ97_13015) pilU 3043941..3045059 (-) 1119 WP_205429920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JWZ97_RS13020 (JWZ97_13020) pilT 3045078..3046112 (-) 1035 WP_205429921.1 type IV pilus twitching motility protein PilT Machinery gene
  JWZ97_RS13025 (JWZ97_13025) - 3046195..3046893 (+) 699 WP_205429923.1 YggS family pyridoxal phosphate-dependent enzyme -
  JWZ97_RS13030 (JWZ97_13030) proC 3046921..3047751 (+) 831 WP_205429925.1 pyrroline-5-carboxylate reductase -
  JWZ97_RS13035 (JWZ97_13035) - 3047748..3048323 (+) 576 WP_205429927.1 YggT family protein -
  JWZ97_RS13040 (JWZ97_13040) - 3048447..3050417 (+) 1971 WP_205429929.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38336.89 Da        Isoelectric Point: 6.6677

>NTDB_id=540185 JWZ97_RS13020 WP_205429921.1 3045078..3046112(-) (pilT) [Methylococcus sp. EFPC2]
MDIAELLAFSVKNGASDLHLSSGLPPMIRVDGDIRRINVPPLEHKEVHALIYDIMNDKQRRDYEEFFETDFSFDLPGVAR
FRVNAFNQDRGAAGVFRTIPSKVLTLEELNCPQFFQDVTRHPRGLILVTGPTGSGKSTTLAAMIDYINSNDYSHILTIED
PIEFVHQSKKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKSMIRSMLSESLQAVIAQTLLKKVAGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGRKDGMQTLDQHL
TELVEKGVVARQTARTRAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=540185 JWZ97_RS13020 WP_205429921.1 3045078..3046112(-) (pilT) [Methylococcus sp. EFPC2]
ATGGATATCGCCGAACTGCTCGCCTTTTCCGTCAAGAACGGGGCCTCCGACTTGCATCTCTCTTCCGGCCTGCCGCCCAT
GATACGTGTGGACGGCGATATTCGGCGCATCAACGTGCCGCCGCTGGAGCACAAGGAAGTACATGCCTTGATCTACGACA
TCATGAACGACAAGCAACGCCGCGATTACGAGGAGTTCTTCGAAACCGACTTTTCCTTCGACCTGCCCGGCGTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGGATCGCGGCGCGGCGGGGGTATTCCGTACCATTCCGTCCAAGGTGCTGACCTTGGA
AGAGTTGAACTGCCCGCAGTTCTTCCAGGATGTCACCCGTCATCCGCGCGGCCTGATCCTGGTGACCGGCCCCACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAGCAACGACTATTCGCATATCCTCACCATCGAGGAC
CCCATCGAGTTCGTGCATCAGAGCAAGAAATCGCTGATCAACCAGCGCGAGGTTCATCGCGACACCCTGGGGTTCAACGA
AGCGCTCCGGTCGGCCCTGCGCGAAGACCCCGATATCATCCTGGTAGGCGAAATGCGCGACCTGGAAACCATACGCCTGG
CGCTAACCGCGGCGGAAACCGGCCATCTCGTATTCGGCACCCTGCACACGACCTCGGCGGCCAAGACCATAGACCGCATC
ATCGACGTGTTCCCCGCCGCCGAAAAAAGCATGATACGTTCCATGCTGTCGGAGTCCCTGCAGGCCGTCATCGCCCAGAC
CCTGCTGAAAAAGGTGGCCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCACGCCTGCCATCCGCAATCTGATAC
GCGAAGACAAGGTCGCGCAGATGTATTCCGCCATACAGACCGGCCGCAAGGACGGCATGCAAACCCTGGATCAGCACCTC
ACCGAGCTCGTCGAAAAAGGCGTCGTCGCGCGTCAGACCGCCCGCACCCGGGCCGTCAACAAGGCCGCCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Pseudomonas aeruginosa PAK

80.769

98.256

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Legionella pneumophila strain Lp02

75.872

100

0.759
  pilT Legionella pneumophila strain ERS1305867

75.872

100

0.759
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Neisseria gonorrhoeae MS11

66.957

100

0.672
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.384

0.395
  pilU Vibrio cholerae strain A1552

39.403

97.384

0.384
  pilU Acinetobacter baylyi ADP1

39.144

95.058

0.372