Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JWZ97_RS13015 Genome accession   NZ_CP070491
Coordinates   3043941..3045059 (-) Length   372 a.a.
NCBI ID   WP_205429920.1    Uniprot ID   A0A974VSC1
Organism   Methylococcus sp. EFPC2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3038941..3050059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWZ97_RS12990 (JWZ97_12990) - 3041038..3041781 (-) 744 WP_205429902.1 S-adenosyl-l-methionine hydroxide adenosyltransferase family protein -
  JWZ97_RS12995 (JWZ97_12995) tatC 3041800..3042609 (-) 810 WP_205429912.1 twin-arginine translocase subunit TatC -
  JWZ97_RS13000 (JWZ97_13000) tatB 3042596..3042892 (-) 297 WP_205429914.1 Sec-independent protein translocase protein TatB -
  JWZ97_RS13005 (JWZ97_13005) tatA 3042907..3043110 (-) 204 WP_205429916.1 twin-arginine translocase TatA/TatE family subunit -
  JWZ97_RS13010 (JWZ97_13010) - 3043223..3043789 (-) 567 WP_205429918.1 DUF2726 domain-containing protein -
  JWZ97_RS13015 (JWZ97_13015) pilU 3043941..3045059 (-) 1119 WP_205429920.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JWZ97_RS13020 (JWZ97_13020) pilT 3045078..3046112 (-) 1035 WP_205429921.1 type IV pilus twitching motility protein PilT Machinery gene
  JWZ97_RS13025 (JWZ97_13025) - 3046195..3046893 (+) 699 WP_205429923.1 YggS family pyridoxal phosphate-dependent enzyme -
  JWZ97_RS13030 (JWZ97_13030) proC 3046921..3047751 (+) 831 WP_205429925.1 pyrroline-5-carboxylate reductase -
  JWZ97_RS13035 (JWZ97_13035) - 3047748..3048323 (+) 576 WP_205429927.1 YggT family protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41686.05 Da        Isoelectric Point: 6.5650

>NTDB_id=540184 JWZ97_RS13015 WP_205429920.1 3043941..3045059(-) (pilU) [Methylococcus sp. EFPC2]
MEFQSLLMLMAHKKASDLFIIPGKEPCLKVDGAIQPIASTKLSADQAKQLIYSIMSQRQIDEFENTKECNFAIAAEGLGR
FRVSAYIQRDTPGVVLRRIETHIPTVEDLNLPPILNELSMTKRGLILFVGATGTGKSTSLAAMVKYRNEHSSGHIITIED
PLEYVHPHAGCIVSQREVGIDTESYEVALKNTLRQAPNVILIGEIRTRETMQQAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQLFMDLSLNMRAIVAQQLIKRADGKGRYPAIEILINTPLASDLIRKGEVHKLKELMKQSREQGMLTFDQA
LYDLYKAGKITYEDALHSADSKNEVRLMIKLGEEGGIEKYSGQHDKMFVVDD

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=540184 JWZ97_RS13015 WP_205429920.1 3043941..3045059(-) (pilU) [Methylococcus sp. EFPC2]
ATGGAATTCCAATCCCTGCTCATGCTCATGGCGCACAAGAAGGCGTCGGACCTGTTCATCATTCCCGGCAAGGAGCCCTG
CCTCAAGGTCGACGGGGCCATACAGCCGATTGCGAGCACCAAGCTCAGTGCCGATCAGGCCAAGCAGCTGATCTACAGCA
TCATGAGTCAACGCCAGATCGACGAGTTCGAAAACACCAAGGAATGCAATTTTGCCATCGCGGCCGAAGGTTTGGGGCGT
TTCCGCGTCAGCGCCTACATCCAGCGCGACACGCCCGGCGTCGTCCTGCGCCGCATCGAGACCCACATTCCCACCGTCGA
GGACTTGAACCTGCCGCCCATCCTCAACGAGTTGTCGATGACCAAGCGTGGTCTGATCCTCTTCGTCGGCGCCACCGGCA
CCGGTAAATCGACCTCGCTGGCCGCCATGGTCAAATACCGCAACGAGCATTCCTCCGGCCATATCATCACCATCGAGGAT
CCACTGGAATACGTGCATCCGCACGCCGGCTGTATCGTCAGCCAGCGCGAGGTGGGCATAGACACGGAATCCTACGAGGT
GGCGCTGAAAAACACGCTGCGACAAGCTCCCAATGTCATCCTGATCGGCGAAATCCGCACCCGCGAAACCATGCAGCAGG
CCATCACCTTCGCCGAGACGGGCCATCTGTGCCTCAGCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
CTGCACTTTTTCCCGGAGGACATGCATCCCCAGTTGTTCATGGACCTGTCCCTGAATATGCGGGCCATCGTCGCTCAGCA
GCTCATCAAGCGGGCCGACGGCAAGGGGCGTTATCCGGCGATCGAGATCCTCATCAATACGCCGCTGGCCTCCGACCTGA
TCCGCAAGGGCGAGGTTCACAAGCTCAAGGAGTTGATGAAACAATCGCGCGAACAAGGTATGTTGACCTTCGATCAGGCG
CTATACGACCTGTACAAGGCAGGCAAGATCACCTACGAAGACGCGCTCCACTCGGCCGATTCCAAGAACGAAGTACGCCT
GATGATCAAGCTGGGCGAGGAAGGCGGCATCGAAAAATACTCCGGCCAGCACGACAAAATGTTCGTGGTCGACGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.949

95.161

0.637
  pilU Acinetobacter baylyi ADP1

64.426

95.968

0.618
  pilU Vibrio cholerae strain A1552

56.857

94.086

0.535
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.954

93.548

0.392
  pilT Legionella pneumophila strain Lp02

39.65

92.204

0.366
  pilT Legionella pneumophila strain ERS1305867

39.65

92.204

0.366
  pilT Pseudomonas aeruginosa PAK

39.528

91.129

0.36