Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C380_RS02215 Genome accession   NC_018708
Coordinates   473945..474988 (-) Length   347 a.a.
NCBI ID   WP_015012257.1    Uniprot ID   A0A8I1V4A6
Organism   Acidovorax sp. KKS102     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 468945..479988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C380_RS02185 (C380_02200) - 469143..469553 (+) 411 WP_015012251.1 YraN family protein -
  C380_RS02190 (C380_02205) - 469644..470243 (+) 600 WP_015012252.1 SIS domain-containing protein -
  C380_RS02195 (C380_02210) - 470240..470905 (+) 666 WP_015012253.1 BON domain-containing protein -
  C380_RS02200 (C380_02215) - 471033..471947 (-) 915 WP_015012254.1 NAD(P)-dependent oxidoreductase -
  C380_RS02205 (C380_02220) pilU 472047..473183 (-) 1137 WP_015012255.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C380_RS02210 (C380_02225) - 473242..473877 (-) 636 WP_015012256.1 Crp/Fnr family transcriptional regulator -
  C380_RS02215 (C380_02230) pilT 473945..474988 (-) 1044 WP_015012257.1 type IV pilus twitching motility protein PilT Machinery gene
  C380_RS02220 (C380_02235) - 475026..475745 (+) 720 WP_015012258.1 YggS family pyridoxal phosphate-dependent enzyme -
  C380_RS02225 (C380_02240) ltaE 475752..476822 (-) 1071 WP_015012259.1 low-specificity L-threonine aldolase -
  C380_RS02230 (C380_02245) - 476932..478176 (-) 1245 WP_015012260.1 branched-chain amino acid ABC transporter substrate-binding protein -
  C380_RS02235 (C380_02250) - 478244..478993 (-) 750 WP_015012261.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38134.90 Da        Isoelectric Point: 6.7743

>NTDB_id=53891 C380_RS02215 WP_015012257.1 473945..474988(-) (pilT) [Acidovorax sp. KKS102]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDALDHKTVHAMVYDIMSDAQRKTYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNYLNESEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LTDLVRRNIISPAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=53891 C380_RS02215 WP_015012257.1 473945..474988(-) (pilT) [Acidovorax sp. KKS102]
GTGGACATTACCCAATTGCTCGCGTTCAGCGTGAAGAACAAGGCCTCCGACCTGCACCTCTCCGCCGGTCTGCCGCCCAT
GATCCGGGTCCACGGCGACGTGCGGCGCATCAACGTCGATGCGCTGGACCACAAGACGGTGCACGCCATGGTGTACGACA
TCATGAGCGACGCGCAGCGCAAGACGTATGAAGAATTCCTGGAGGTGGACTTTTCGTTCGAGATCGAAGGCCTGGCCCGT
TTCCGCGTCAACGCCTTCAACCAGAACCGGGGCGCGGCCGCCGTGTTCCGGACGATTCCAAGCAAGATCCTCACGCTGGA
ACAGCTCAACGCCCCCAAGATCTTCGGCGACCTGGCCTTGAAGCCACGGGGCCTGGTGCTGGTGACCGGCCCCACGGGCT
CGGGCAAGTCCACCACGCTGGCCGCCATGGTCAACTACCTCAACGAATCCGAGTACGGCCACATCCTCACAGTGGAAGAC
CCGATCGAATTCGTGCACGAGTCCAAGAAGTGCCTGATCAACCAGCGTGAAGTCGGCCCGATGACGCTGTCGTTCGCGGC
GGCCCTGAAGTCTGCACTGCGTGAAGACCCGGACGCCATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATCCGTCTGG
CCATGACAGCGGCGGAAACGGGCCACTTGGTGTTTGGCACGCTGCACACCTCCAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTCCCCGCCGAAGAAAAGGAAATGGTCCGTGCGATGTTGTCCGAATCGCTGCAGGCCGTGATCTCGCAGAC
GCTGTGCAAGACCAAGGATGGCTCCGGGCGCGTGGCCGCGCACGAGATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAGGCCAAGGTTGCGCAGATGTATTCGACCATCCAGACCAGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTGACCGACCTGGTGCGCCGCAACATCATCAGCCCGGCCGAAGCACGCAGCAAGGCCAAGATCCCCGAGAACTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.522

99.424

0.761
  pilT Acinetobacter baumannii D1279779

76.522

99.424

0.761
  pilT Acinetobacter baumannii strain A118

76.522

99.424

0.761
  pilT Pseudomonas stutzeri DSM 10701

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.362

99.424

0.749
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Neisseria meningitidis 8013

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.742

98.271

0.666
  pilT Vibrio cholerae strain A1552

67.742

98.271

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment