Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C380_RS02205 Genome accession   NC_018708
Coordinates   472047..473183 (-) Length   378 a.a.
NCBI ID   WP_015012255.1    Uniprot ID   A0A8I1V4W1
Organism   Acidovorax sp. KKS102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 467047..478183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C380_RS02180 (C380_02195) rsmI 468214..469143 (-) 930 WP_015012250.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  C380_RS02185 (C380_02200) - 469143..469553 (+) 411 WP_015012251.1 YraN family protein -
  C380_RS02190 (C380_02205) - 469644..470243 (+) 600 WP_015012252.1 SIS domain-containing protein -
  C380_RS02195 (C380_02210) - 470240..470905 (+) 666 WP_015012253.1 BON domain-containing protein -
  C380_RS02200 (C380_02215) - 471033..471947 (-) 915 WP_015012254.1 NAD(P)-dependent oxidoreductase -
  C380_RS02205 (C380_02220) pilU 472047..473183 (-) 1137 WP_015012255.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  C380_RS02210 (C380_02225) - 473242..473877 (-) 636 WP_015012256.1 Crp/Fnr family transcriptional regulator -
  C380_RS02215 (C380_02230) pilT 473945..474988 (-) 1044 WP_015012257.1 type IV pilus twitching motility protein PilT Machinery gene
  C380_RS02220 (C380_02235) - 475026..475745 (+) 720 WP_015012258.1 YggS family pyridoxal phosphate-dependent enzyme -
  C380_RS02225 (C380_02240) ltaE 475752..476822 (-) 1071 WP_015012259.1 low-specificity L-threonine aldolase -
  C380_RS02230 (C380_02245) - 476932..478176 (-) 1245 WP_015012260.1 branched-chain amino acid ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41888.30 Da        Isoelectric Point: 6.9078

>NTDB_id=53890 C380_RS02205 WP_015012255.1 472047..473183(-) (pilU) [Acidovorax sp. KKS102]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGKVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMISQRLIPKQDGKGRAAAVEVMLNTPLISDLIFKGEVSEIKEIMKKSRNLG
MQTFDQALFDAYEANVISYEDALRNADSLNDLRLQIKLNSQRAKSPDLAQGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=53890 C380_RS02205 WP_015012255.1 472047..473183(-) (pilU) [Acidovorax sp. KKS102]
ATGGAACGCGATCAGGCCAGTAAATTCATTAATGACCTGCTCAAGCTGATGGTGAGCCGCAATGGCAGCGACTTGTTCAT
CACGGCAGAGTTTCCACCCGCCATCAAGGTCGATGGCAAGGTGACCAAGGTGTCGCCGCAGCCGCTCACACCCAACCACA
CGCTGACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACGAAAGAGTGCAACTTCGCCATC
TCGCCCGCCGGCATCGGGCGCTTCCGCGTCAACGCCTTCATCCAGCAGGGCAAGGTGGGTATGGTGCTGCGGACCATTCC
GCTCACACTGCCGACCATTGACGGCCTGGGCGTGCCACAGGTGCTCAAGGAGGTGACGATGACCAAACGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAATCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCGTTCGGC
CACATCATCACGGTGGAAGACCCTATCGAATTTGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCCT
GGACACCGACAGCTGGGAAGCCGCCCTGAAGAACACGTTGCGGCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACATGCGGTGGCGTTTGCCGAAACCGGGCACTTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCTGAAGAGCGCCGCGCACAATTGCTGATGGACCTGTCGCTCAACCT
GCGCGCCATGATCTCGCAACGCCTGATCCCCAAGCAGGACGGCAAGGGCCGCGCCGCAGCGGTGGAGGTGATGCTCAACA
CCCCGCTGATCTCCGACCTGATCTTCAAGGGCGAGGTCTCCGAAATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATGCCTACGAGGCCAACGTCATCAGCTACGAAGACGCGCTGCGCAATGCCGA
CTCGCTCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGCGCCAAGTCGCCCGACCTGGCGCAGGGCACCGAGC
ATTTCGCGATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

58.939

94.709

0.558
  pilU Vibrio cholerae strain A1552

54.31

92.063

0.5
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

44.444

88.095

0.392
  pilT Acinetobacter baumannii D1279779

44.444

88.095

0.392
  pilT Acinetobacter baumannii strain A118

44.444

88.095

0.392
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Legionella pneumophila strain ERS1305867

43.844

88.095

0.386
  pilT Legionella pneumophila strain Lp02

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baylyi ADP1

43.887

84.392

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.849

83.862

0.368
  pilT Vibrio cholerae strain A1552

43.849

83.862

0.368

Multiple sequence alignment