Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q91_RS00370 Genome accession   NC_018697
Coordinates   79955..80992 (-) Length   345 a.a.
NCBI ID   WP_015004887.1    Uniprot ID   A0AB33Z1F9
Organism   Cycloclasticus sp. P1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 74955..85992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q91_RS00345 (Q91_0069) - 75493..76806 (-) 1314 WP_041230466.1 PilX N-terminal domain-containing pilus assembly protein -
  Q91_RS00350 (Q91_0070) - 76812..77471 (-) 660 WP_015004883.1 PilW family protein -
  Q91_RS11680 (Q91_0071) - 77462..77986 (-) 525 WP_015004884.1 GspH/FimT family pseudopilin -
  Q91_RS11820 (Q91_0072) - 78161..78559 (+) 399 WP_015004885.1 type IV pilin protein -
  Q91_RS00365 (Q91_0073) pilU 78803..79930 (-) 1128 WP_015004886.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q91_RS00370 (Q91_0074) pilT 79955..80992 (-) 1038 WP_015004887.1 type IV pilus twitching motility protein PilT Machinery gene
  Q91_RS00375 (Q91_0075) - 81116..81805 (+) 690 WP_015004888.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q91_RS00380 (Q91_0076) proC 81839..82666 (+) 828 WP_015004889.1 pyrroline-5-carboxylate reductase -
  Q91_RS00385 (Q91_0077) - 82666..83220 (+) 555 WP_015004890.1 YggT family protein -
  Q91_RS00390 (Q91_0078) - 83455..85386 (+) 1932 WP_015004891.1 dynamin family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38628.40 Da        Isoelectric Point: 6.4346

>NTDB_id=53777 Q91_RS00370 WP_015004887.1 79955..80992(-) (pilT) [Cycloclasticus sp. P1]
MDISDLLAFSTKNNASDLHLSAGLPPMIRVDGDIRRINMPELEHKEVHAMIYDIMNDKQRRDFEEFLEVDFSFELPGTAR
FRVNAFNQDRGAAAVFRTIPVDILTLEDLNCPQSFKEIINVPRGIVLVTGPTGSGKSTTLAALMDYKNDNVYEHILTIED
PIEFVHKSKKCLINQREVHKHTHGFSEALRVALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPEGEKGMVRSMLSESLRAVIAQTLMKKIGGGRIAAWEIMTGTAAIRNLIREDKIAQMYSTIQTSRKDGMQTLDQHL
TELVQAGVITRQDARMKAADKKAFS

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=53777 Q91_RS00370 WP_015004887.1 79955..80992(-) (pilT) [Cycloclasticus sp. P1]
ATGGATATTTCAGATTTATTAGCCTTTAGCACCAAAAATAACGCCTCTGACTTACACCTTTCAGCTGGTTTACCTCCTAT
GATTCGAGTGGACGGCGACATTCGACGAATCAATATGCCTGAGCTTGAGCATAAAGAAGTTCATGCGATGATTTATGACA
TCATGAACGATAAACAGCGCCGTGACTTTGAAGAGTTTCTTGAAGTCGATTTTTCCTTCGAGTTGCCCGGCACTGCTCGC
TTCCGTGTTAATGCCTTTAATCAAGACCGTGGAGCTGCTGCTGTATTTAGAACTATTCCCGTTGATATTCTTACCCTAGA
AGACTTAAATTGCCCCCAATCTTTTAAAGAAATCATCAATGTTCCTCGTGGCATTGTGCTGGTAACAGGGCCAACAGGGT
CAGGAAAATCGACCACACTAGCGGCTTTGATGGATTATAAAAACGATAATGTATACGAACACATTCTCACCATTGAAGAC
CCTATCGAATTCGTACATAAAAGTAAAAAATGCCTTATTAATCAACGTGAAGTACACAAGCATACACATGGGTTTAGTGA
AGCCTTACGTGTTGCGCTTCGTGAAGACCCCGACATTATTCTAGTCGGCGAAATGCGAGACCTTGAAACCATTCGCCTTG
CGTTAACCGCGGCAGAAACAGGCCACTTGGTCTTCGGCACTTTACACACCAGCTCTGCTGCCAAAACCATTGACCGTATT
ATTGATGTATTCCCCGAGGGTGAAAAAGGCATGGTTCGCTCCATGTTATCCGAGTCACTTCGTGCTGTTATTGCACAAAC
CTTAATGAAAAAAATTGGTGGTGGACGAATTGCCGCATGGGAAATCATGACCGGCACTGCGGCTATCCGAAACCTCATTC
GAGAAGATAAAATTGCACAAATGTATTCCACCATACAAACCAGTCGTAAAGATGGTATGCAAACCCTCGATCAACACTTA
ACAGAGCTTGTTCAAGCCGGTGTCATTACAAGACAAGATGCCCGAATGAAAGCCGCCGACAAAAAAGCATTTAGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.744

99.71

0.765
  pilT Acinetobacter nosocomialis M2

75.872

99.71

0.757
  pilT Acinetobacter baumannii D1279779

75.872

99.71

0.757
  pilT Acinetobacter baumannii strain A118

75.872

99.71

0.757
  pilT Pseudomonas stutzeri DSM 10701

75.581

99.71

0.754
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Acinetobacter baylyi ADP1

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.536

100

0.675
  pilT Vibrio cholerae strain A1552

67.536

100

0.675
  pilT Neisseria meningitidis 8013

65.029

100

0.652
  pilT Neisseria gonorrhoeae MS11

64.74

100

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.422

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Vibrio cholerae strain A1552

39.883

98.841

0.394
  pilU Acinetobacter baylyi ADP1

39.766

99.13

0.394

Multiple sequence alignment