Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q91_RS00365 Genome accession   NC_018697
Coordinates   78803..79930 (-) Length   375 a.a.
NCBI ID   WP_015004886.1    Uniprot ID   A0AB33Z1A0
Organism   Cycloclasticus sp. P1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 73803..84930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q91_RS00345 (Q91_0069) - 75493..76806 (-) 1314 WP_041230466.1 PilX N-terminal domain-containing pilus assembly protein -
  Q91_RS00350 (Q91_0070) - 76812..77471 (-) 660 WP_015004883.1 PilW family protein -
  Q91_RS11680 (Q91_0071) - 77462..77986 (-) 525 WP_015004884.1 GspH/FimT family pseudopilin -
  Q91_RS11820 (Q91_0072) - 78161..78559 (+) 399 WP_015004885.1 type IV pilin protein -
  Q91_RS00365 (Q91_0073) pilU 78803..79930 (-) 1128 WP_015004886.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q91_RS00370 (Q91_0074) pilT 79955..80992 (-) 1038 WP_015004887.1 type IV pilus twitching motility protein PilT Machinery gene
  Q91_RS00375 (Q91_0075) - 81116..81805 (+) 690 WP_015004888.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q91_RS00380 (Q91_0076) proC 81839..82666 (+) 828 WP_015004889.1 pyrroline-5-carboxylate reductase -
  Q91_RS00385 (Q91_0077) - 82666..83220 (+) 555 WP_015004890.1 YggT family protein -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41741.52 Da        Isoelectric Point: 5.0659

>NTDB_id=53776 Q91_RS00365 WP_015004886.1 78803..79930(-) (pilU) [Cycloclasticus sp. P1]
MDFDALLQLVVHKKASDLFITSGWAPTIKVNGALQQVSKTRLSASQSLDIVKSIMTDDQKKEFEETKECQFAIQRPDIGR
FRVSAFIQKDDAGMVLRRIETTIPTSEELNLPPILDELAMTKRGLIIFVGATGTGKSTSLAAMVGYRNRNSSGHIITIED
PVEYVHEHAGCVITQREVGIDTESYEVALKNTLRQAPDVILIGEIRSRETMEHAMTFAETGHLCLSTLHANNANQALDRI
IHFFPEEAHKQLFMDLSLNLKAMIAQQLVPTIDGKGRKAVIEIMLNTPLASDFIRKGDIHKLKELMKNSTEQGMQTFDQA
LYGFYASGDISYENAINYADSSNEVRLMIKLGDAKAKNPDDDNDSGMELIDNYDG

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=53776 Q91_RS00365 WP_015004886.1 78803..79930(-) (pilU) [Cycloclasticus sp. P1]
ATGGATTTTGATGCACTTTTACAACTCGTTGTTCATAAAAAAGCATCTGACTTATTTATTACATCTGGTTGGGCTCCCAC
CATTAAGGTCAATGGAGCGCTACAGCAAGTATCAAAAACACGCTTAAGCGCTAGCCAGTCTTTAGACATCGTAAAAAGCA
TCATGACCGATGATCAGAAAAAAGAGTTTGAAGAGACCAAAGAGTGCCAATTTGCCATTCAACGCCCAGATATTGGCCGT
TTTCGTGTCAGCGCATTTATACAAAAAGACGATGCTGGCATGGTCTTACGTCGAATCGAAACCACCATCCCAACCTCTGA
AGAACTCAACCTTCCACCAATTTTAGACGAATTGGCAATGACAAAACGCGGGCTCATTATCTTCGTGGGCGCAACCGGCA
CCGGTAAATCAACGTCCTTAGCTGCGATGGTTGGCTACCGTAATAGAAACAGTTCCGGCCATATCATTACCATTGAAGAC
CCTGTTGAGTATGTACATGAACACGCGGGGTGTGTCATTACGCAACGCGAAGTAGGCATAGATACCGAATCGTATGAAGT
TGCGCTTAAAAACACGTTGCGTCAAGCACCCGATGTCATCCTAATTGGTGAGATTCGTAGTCGCGAAACCATGGAGCACG
CTATGACCTTCGCTGAAACGGGGCATTTATGCCTGTCCACTTTGCACGCTAATAACGCCAACCAAGCACTTGATCGTATT
ATTCATTTCTTCCCTGAAGAAGCGCACAAGCAATTATTTATGGATTTATCGCTCAACCTAAAGGCCATGATCGCGCAGCA
ACTTGTACCCACCATCGATGGCAAAGGCAGAAAAGCAGTGATTGAAATTATGCTTAACACACCGTTAGCCTCTGACTTCA
TCAGAAAAGGTGATATTCATAAACTAAAAGAGCTGATGAAAAACTCTACCGAACAAGGCATGCAAACGTTTGACCAGGCA
CTGTACGGCTTCTATGCCAGCGGCGACATCAGCTACGAAAATGCCATTAATTACGCCGACTCCAGCAACGAGGTACGCTT
AATGATCAAGCTGGGTGATGCAAAAGCTAAAAACCCCGATGATGACAACGACAGTGGTATGGAGTTGATTGATAATTATG
ACGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.898

93.867

0.637
  pilU Acinetobacter baylyi ADP1

62.169

100

0.627
  pilU Vibrio cholerae strain A1552

57.429

93.333

0.536
  pilT Legionella pneumophila strain ERS1305867

41.935

90.933

0.381
  pilT Legionella pneumophila strain Lp02

41.935

90.933

0.381
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

90.4

0.373
  pilT Pseudomonas aeruginosa PAK

41.194

89.333

0.368
  pilT Acinetobacter baylyi ADP1

40.176

90.933

0.365
  pilT Vibrio cholerae strain A1552

41.768

87.467

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.768

87.467

0.365
  pilT Acinetobacter baumannii strain A118

40.299

89.333

0.36
  pilT Pseudomonas stutzeri DSM 10701

40.299

89.333

0.36
  pilT Acinetobacter baumannii D1279779

40.299

89.333

0.36
  pilT Neisseria meningitidis 8013

39.474

91.2

0.36
  pilT Neisseria gonorrhoeae MS11

39.474

91.2

0.36

Multiple sequence alignment