Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B5T_RS00445 Genome accession   NC_018691
Coordinates   94521..95555 (-) Length   344 a.a.
NCBI ID   WP_014992459.1    Uniprot ID   -
Organism   Alloalcanivorax dieselolei B5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 89521..100555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5T_RS00420 (B5T_00084) - 89811..90419 (+) 609 WP_014992454.1 FMN-dependent NADH-azoreductase -
  B5T_RS00425 (B5T_00085) - 90468..91472 (+) 1005 WP_014992455.1 glutathione S-transferase family protein -
  B5T_RS00430 (B5T_00086) - 91513..92367 (+) 855 WP_014992456.1 pirin family protein -
  B5T_RS00435 (B5T_00087) - 92452..92766 (-) 315 WP_014992457.1 HigA family addiction module antitoxin -
  B5T_RS00440 (B5T_00088) pilU 93381..94502 (-) 1122 WP_014992458.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  B5T_RS00445 (B5T_00089) pilT 94521..95555 (-) 1035 WP_014992459.1 type IV pilus twitching motility protein PilT Machinery gene
  B5T_RS00450 (B5T_00090) - 95674..96378 (+) 705 WP_041716608.1 YggS family pyridoxal phosphate-dependent enzyme -
  B5T_RS00455 (B5T_00091) proC 96411..97223 (+) 813 WP_014992461.1 pyrroline-5-carboxylate reductase -
  B5T_RS00460 (B5T_00092) - 97600..98148 (+) 549 WP_014992462.1 YggT family protein -
  B5T_RS00465 (B5T_00093) - 98227..99150 (-) 924 WP_014992463.1 hydroxymethylglutaryl-CoA lyase -
  B5T_RS00470 (B5T_00094) - 99337..100188 (+) 852 WP_041716610.1 triacylglycerol lipase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38001.62 Da        Isoelectric Point: 6.5061

>NTDB_id=53739 B5T_RS00445 WP_014992459.1 94521..95555(-) (pilT) [Alloalcanivorax dieselolei B5]
MDITELLAFSAKNGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKEVHSLIYEIMNDKQRKDYEEFLETDFSFDVPSVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEELGMGKVFQNIASLPRGIVLVTGPTGSGKSTTLAAMMDYINNTRYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVVSQTLMKKNGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGASVGMQTLDQCL
QGLVQRGLVSREVAREKAKSPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=53739 B5T_RS00445 WP_014992459.1 94521..95555(-) (pilT) [Alloalcanivorax dieselolei B5]
ATGGATATCACCGAATTGCTCGCCTTCAGCGCCAAAAACGGCGCTTCGGATCTGCACCTGTCCGCCGGCCTGCCGCCGAT
GATTCGCGTGGACGGCGACGTGCGCCGCATCAATCTGCCGCCCATGGAGCACAAGGAAGTGCACTCCCTGATCTACGAGA
TCATGAACGACAAGCAGCGCAAGGACTACGAGGAGTTCCTGGAAACCGACTTCTCCTTCGATGTGCCCAGCGTGGCGCGT
TTCCGGGTCAACGCCTTCAATCAGAATCGGGGCGCCGGCGCCGTATTCCGGACCATTCCCTCCAAGGTGCTGAGCATGGA
GGAACTGGGCATGGGCAAGGTGTTCCAGAACATCGCCTCGCTGCCGCGCGGCATCGTGCTGGTTACCGGCCCCACCGGCT
CCGGTAAGTCCACCACGCTGGCGGCGATGATGGATTACATCAATAACACCCGCTACGAACACATCCTCACCATTGAGGAC
CCGATTGAATTCGTCCATGAATCGAAGAAATGTCTGGTCAACCAGCGGGAAGTGCACCGGGACACCCATGGTTTCGCCGA
AGCCCTGCGCTCGGCGCTGCGGGAGGATCCGGACATCATCCTGGTGGGGGAGATGCGGGACCTGGAGACCATTCGTCTGG
CGCTGACCGCCGCCGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACCAGCGCCGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGCCGAGGAGAAGGCCATGGTCCGTTCCATGCTCTCCGAGTCGCTGCAGGCGGTGGTGTCGCAAAC
GCTGATGAAGAAAAACGGCGGCGGCCGGGTGGCCGCCCACGAAATCATGATCGGCACCCCGGCGATTCGAAACCTGATTC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGCGCCTCGGTGGGCATGCAGACCCTGGATCAATGCCTG
CAAGGACTGGTGCAGCGGGGGCTGGTATCCCGCGAAGTGGCCCGGGAAAAAGCCAAGTCCCCGGACTCAATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

86.919

100

0.869
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baumannii D1279779

84.302

100

0.843
  pilT Acinetobacter baumannii strain A118

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

81.395

100

0.814
  pilT Legionella pneumophila strain Lp02

75.74

98.256

0.744
  pilT Legionella pneumophila strain ERS1305867

75.74

98.256

0.744
  pilT Neisseria meningitidis 8013

69.565

100

0.698
  pilT Neisseria gonorrhoeae MS11

68.986

100

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.339

99.419

0.52
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

39.71

100

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387

Multiple sequence alignment