Detailed information NTG0053738

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B5T_RS00440
• Genome accession: NC_018691
• Coordinates: 93381..94502 (-)
• Length: 373 a.a.
• NCBI ID: WP_014992458.1
• Uniprot ID: K0C9J6
• Organism: Alloalcanivorax dieselolei B5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 88381..99502

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5T_RS00415 (B5T_00083)	-	88820..89713 (-)	894	WP_014992453.1	LysR family transcriptional regulator	-
B5T_RS00420 (B5T_00084)	-	89811..90419 (+)	609	WP_014992454.1	FMN-dependent NADH-azoreductase	-
B5T_RS00425 (B5T_00085)	-	90468..91472 (+)	1005	WP_014992455.1	glutathione S-transferase family protein	-
B5T_RS00430 (B5T_00086)	-	91513..92367 (+)	855	WP_014992456.1	pirin family protein	-
B5T_RS00435 (B5T_00087)	-	92452..92766 (-)	315	WP_014992457.1	HigA family addiction module antitoxin	-
B5T_RS00440 (B5T_00088)	pilU	93381..94502 (-)	1122	WP_014992458.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B5T_RS00445 (B5T_00089)	pilT	94521..95555 (-)	1035	WP_014992459.1	type IV pilus twitching motility protein PilT	Machinery gene
B5T_RS00450 (B5T_00090)	-	95674..96378 (+)	705	WP_041716608.1	YggS family pyridoxal phosphate-dependent enzyme	-
B5T_RS00455 (B5T_00091)	proC	96411..97223 (+)	813	WP_014992461.1	pyrroline-5-carboxylate reductase	-
B5T_RS00460 (B5T_00092)	-	97600..98148 (+)	549	WP_014992462.1	YggT family protein	-
B5T_RS00465 (B5T_00093)	-	98227..99150 (-)	924	WP_014992463.1	hydroxymethylglutaryl-CoA lyase	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41591.80 Da
• Isoelectric Point: 6.7399

>NTDB_id=53738 B5T_RS00440 WP_014992458.1 93381..94502(-) (pilU) [Alloalcanivorax dieselolei B5]
MEFEDLLKLMVQKGGSDLFITAGVPPSMKVHGKVVPVTRNALTPDMTRDLVFGVMSEKQRKEFVDQHECNFALSSRGIGR
FRVSAFYQRNLVGMVLRRIETKIPDVDELRLPPIIKDLAMTKRGLVIFVGATGTGKSTSLASMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGLDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIAFAETGHLCLATLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLRGIIAQQLIPTPDGKGRRAAIEVLLNTPLVADHIRKGEVHLLKALMGKSREQGMQTFDQA
LYDLYDAGEITYEDAIAHADSPNDLRLMIKLANETDASSLDHATRGLSIEDSD

Nucleotide

• Length: 1122 bp

>NTDB_id=53738 B5T_RS00440 WP_014992458.1 93381..94502(-) (pilU) [Alloalcanivorax dieselolei B5]
ATGGAATTCGAAGACCTGCTCAAACTGATGGTGCAAAAAGGCGGCTCGGACCTGTTCATCACCGCCGGTGTCCCGCCAAG
TATGAAAGTCCACGGCAAGGTGGTGCCGGTTACCCGTAACGCCCTGACCCCGGACATGACCCGGGACCTGGTGTTCGGTG
TGATGTCGGAAAAACAGCGCAAGGAGTTCGTCGACCAGCACGAATGCAACTTCGCGCTGTCCTCGCGGGGCATCGGCCGC
TTCCGCGTTTCCGCCTTCTATCAGCGGAACCTGGTGGGCATGGTGTTGCGGCGCATCGAGACCAAAATTCCCGATGTGGA
CGAGCTGCGGCTGCCGCCGATCATCAAGGACCTGGCGATGACCAAGCGCGGCCTGGTGATTTTCGTCGGCGCCACCGGCA
CCGGGAAATCCACATCGCTGGCGTCGATGATCGGCCACCGTAATCGCAATTCCAAAGGCCACATCATCTCTATTGAGGAT
CCGATCGAATTCATCCACCAGCACCAGGGCTGCATTGTTACACAACGGGAAGTGGGTCTGGATACGGACAGTTTCGAGAT
CGCTCTCAAGAACACCCTGCGACAGGCGCCGGATGTGATTCTGATCGGTGAGATCCGCAGCCGCGAGGTGATGGATTACG
CCATCGCTTTCGCCGAAACCGGCCATCTGTGCCTGGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATTCACTTTTTCCCGGCCGACCGTCACCGGCAGTTATGGATGGACCTCTCCCTGAACCTGCGCGGCATCATCGCCCAACA
ACTGATCCCGACTCCGGACGGCAAGGGCCGCCGCGCCGCCATCGAAGTGCTGCTGAACACGCCACTGGTGGCGGACCATA
TCCGTAAAGGCGAAGTGCATTTGCTCAAGGCGCTGATGGGCAAATCCCGGGAGCAGGGCATGCAGACTTTCGATCAGGCC
CTCTATGATCTTTATGACGCCGGTGAAATCACTTACGAGGACGCGATTGCCCACGCCGACTCCCCCAACGATCTGCGTCT
GATGATCAAGCTCGCCAACGAAACCGACGCCTCCAGCCTGGACCACGCCACCCGTGGCCTGAGCATCGAGGACTCGGACT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (116-269)

3D structure

• AlphaFold DB: K0C9J6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	76.676	100	0.767
pilU	Acinetobacter baylyi ADP1	67.655	99.464	0.673
pilU	Vibrio cholerae strain A1552	56.546	96.247	0.544
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.017	95.71	0.402