Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMEC673_RS14925 Genome accession   NC_018678
Coordinates   3440493..3441536 (-) Length   347 a.a.
NCBI ID   WP_014950464.1    Uniprot ID   -
Organism   Alteromonas macleodii str. 'English Channel 673'     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3435493..3446536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMEC673_RS14895 (AMEC673_14660) - 3435928..3436656 (+) 729 WP_014977239.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AMEC673_RS14900 (AMEC673_14665) gshB 3436795..3437748 (+) 954 WP_014950459.1 glutathione synthase -
  AMEC673_RS14905 (AMEC673_14670) - 3437877..3438443 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  AMEC673_RS14910 (AMEC673_14675) ruvX 3438436..3438867 (+) 432 WP_014950461.1 Holliday junction resolvase RuvX -
  AMEC673_RS14915 (AMEC673_14680) - 3438914..3439231 (-) 318 WP_014977240.1 putative signal transducing protein -
  AMEC673_RS14920 (AMEC673_14685) pilU 3439372..3440484 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMEC673_RS14925 (AMEC673_14690) pilT 3440493..3441536 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  AMEC673_RS14930 (AMEC673_14695) - 3441564..3442250 (+) 687 WP_014977242.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMEC673_RS14935 (AMEC673_14700) proC 3442321..3443142 (+) 822 WP_014950466.1 pyrroline-5-carboxylate reductase -
  AMEC673_RS14940 (AMEC673_14705) - 3443163..3443699 (+) 537 WP_014977243.1 YggT family protein -
  AMEC673_RS14945 (AMEC673_14710) - 3443796..3444269 (+) 474 WP_014977244.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38380.09 Da        Isoelectric Point: 6.5677

>NTDB_id=53698 AMEC673_RS14925 WP_014950464.1 3440493..3441536(-) (pilT) [Alteromonas macleodii str. 'English Channel 673']
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHGLVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAAKSVDKQSMNSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=53698 AMEC673_RS14925 WP_014950464.1 3440493..3441536(-) (pilT) [Alteromonas macleodii str. 'English Channel 673']
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCGGGCCTACCACCAAT
AATTCGTGTAGATGGCGAAATGCGTAAACTGAATGTGCCAGCCCTCGATCACAAGCAAGTACATGGTCTCGTTTATGAGA
TTATGAATGACATGCAGCGCAAAGAATACGAAGAAAACCTGGAAACCGATTTTTCTTTCGAAGTGAATGGCTTGTCGCGC
TTTCGTGTTAACGCGTTTGTGCAAAACCGAGGCGCCGCGGCAGTATTACGTACTATCCCCAGTAAAGTATTAACCTTAGA
CGACCTTGGCGCGCCTGAAATTTTCAAGGAAATTATAAATCAGCCTACCGGCATTGTACTTGTGACAGGTGCCACCGGCT
CGGGTAAAAGTACCACGCTTGCGGCCATGGTCGACCATATCAACACCCACAAGCGTGAACATATTCTCACCATTGAAGAC
CCTATCGAATTCGTGCACGACAACAAGCTTAGCTTGGTAAACCAGCGGGAAGTACACCGCGATACGCACTCGTTTAATAA
CGCACTGCGCTCGGCGCTTCGTGAAGACCCAGACGTAATTCTAGTCGGTGAGCTACGTGACTTAGAAACCATACGCCTTG
CCATTTCAGCTGCCGAAACCGGCCATTTAGTATTCGGCACTCTTCACACCAACTCCGCACCAAAAACCATTGACCGTATT
ATCGATGTTTTCCCGGCAGAAGAAAAAGCCATGGTGCGTTCTATGTTGTCGGAATCATTGCGTGCGGTTATTTCACAGAC
ACTGCTTAAGAAAGTAGGTGGGGGCCGAGTTGCGGCTCATGAAATCATGGTGGGGATCCCCGCTATTCGTAACCTTATTC
GAGAAGATAAAGTGCCGCAAATGTACTCGGTTATTCAAACGGGCCAGGCTCACGGTATGCAAACTATGGATCAGTGTCTT
CAGCGCTTAGTGGCGATGGGGGTTATCACCAAACAAGACGCCGCAGCAAAGTCAGTTGATAAACAGTCGATGAACAGTTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter nosocomialis M2

75.581

99.135

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

74.852

97.406

0.729
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

65.029

99.712

0.648
  pilT Neisseria gonorrhoeae MS11

64.74

99.712

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

98.847

0.513
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

41.42

97.406

0.403

Multiple sequence alignment