Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AMEC673_RS14920 Genome accession   NC_018678
Coordinates   3439372..3440484 (-) Length   370 a.a.
NCBI ID   WP_014977241.1    Uniprot ID   A0AB33A1W2
Organism   Alteromonas macleodii str. 'English Channel 673'     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3434372..3445484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMEC673_RS14895 (AMEC673_14660) - 3435928..3436656 (+) 729 WP_014977239.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AMEC673_RS14900 (AMEC673_14665) gshB 3436795..3437748 (+) 954 WP_014950459.1 glutathione synthase -
  AMEC673_RS14905 (AMEC673_14670) - 3437877..3438443 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  AMEC673_RS14910 (AMEC673_14675) ruvX 3438436..3438867 (+) 432 WP_014950461.1 Holliday junction resolvase RuvX -
  AMEC673_RS14915 (AMEC673_14680) - 3438914..3439231 (-) 318 WP_014977240.1 putative signal transducing protein -
  AMEC673_RS14920 (AMEC673_14685) pilU 3439372..3440484 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMEC673_RS14925 (AMEC673_14690) pilT 3440493..3441536 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  AMEC673_RS14930 (AMEC673_14695) - 3441564..3442250 (+) 687 WP_014977242.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMEC673_RS14935 (AMEC673_14700) proC 3442321..3443142 (+) 822 WP_014950466.1 pyrroline-5-carboxylate reductase -
  AMEC673_RS14940 (AMEC673_14705) - 3443163..3443699 (+) 537 WP_014977243.1 YggT family protein -
  AMEC673_RS14945 (AMEC673_14710) - 3443796..3444269 (+) 474 WP_014977244.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40781.69 Da        Isoelectric Point: 6.0487

>NTDB_id=53697 AMEC673_RS14920 WP_014977241.1 3439372..3440484(-) (pilU) [Alteromonas macleodii str. 'English Channel 673']
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTESATSEEASLALVHEAMTEKQQNEFHTTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=53697 AMEC673_RS14920 WP_014977241.1 3439372..3440484(-) (pilU) [Alteromonas macleodii str. 'English Channel 673']
ATGTTAGATTCACTACTACAAAAAATGGTCGATACCAACGCATCGGATTTATTCGTAACGGCGGGGTTTCCGGTAAGTGC
CAAAATTAACGGCAAGCTTACGCCAGTGACGGAAAGCGCGACCTCGGAAGAGGCATCGCTGGCGCTGGTTCACGAAGCGA
TGACTGAAAAACAGCAGAATGAGTTTCACACGACCAAAGAATGTAATTTCGCCATAGTACGAGAAGGCATTGGTCGTTTC
CGCTGCTCTGCATTTTGGCAGCGTGACCAAGCGGGCATGGTGATTCGCCGCATCGTTACTCAAATTCCGAAAGCTGATGA
TTTGGGTCTGCCGCGAGTATTAAAAGAAATTATTATGGCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGCACGG
GTAAGTCCACGTCATTGGCTGCGCTTATTGGGCATAGAAATGAAAATTCGCACGGGCATATACTGACTATTGAAGACCCC
ATTGAATTTGTGCACGAGCATAAAAACTGTGTTATTACCCAGCGCGAGGTGGGCATAGATACTCAGTCTTTCGACGACGC
CCTTAAAAGTTCGCTACGTCAGGCACCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAAACCATGGAATATGCCA
TGTCGTTTGCTGATACAGGGCATTTGTGTGTGGCTACGCTGCACGCTAACAACGCGAACCAAGCTATTGAACGTATTATG
CATTTAGCGCCGAAAGATCAGCACGATAAGTTGCGCTTTGACTTAAGCTTAAACATCCGCGCCATTGTTGCGCAGCAACT
AGTACCTACGCCAGACGGCAAGGGTAGGGTTGCGGCAATAGAAATATTGCTAAACTCGCCGCTAGTGCGCGACCTAATAC
AGCGCAATGAAATAGGCGCGTTAAAAGAAGCAATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCGCTG
TATGATTTGTACAAAGCGGGTAAGATTGATATGGACCAAGCGCTGCACCACGCCGACTCGCCTAATGACCTGCGCTTAAT
GATCAAGCTTGATACCAGCGACGGTTCTAGCTTAGGTACACTGTCAAATGTATCTATTGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilU Vibrio cholerae strain A1552

56.25

99.459

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

91.892

0.392
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362

Multiple sequence alignment