Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JSQ74_RS10440 Genome accession   NZ_CP069710
Coordinates   2171377..2172357 (-) Length   326 a.a.
NCBI ID   WP_204084397.1    Uniprot ID   -
Organism   Escherichia coli strain EA2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2166377..2177357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JSQ74_RS10410 (JSQ74_10410) yggI 2167040..2167537 (+) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -
  JSQ74_RS10415 (JSQ74_10415) endA 2167632..2168339 (+) 708 WP_000286500.1 deoxyribonuclease I -
  JSQ74_RS10420 (JSQ74_10420) rsmE 2168419..2169150 (+) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  JSQ74_RS10425 (JSQ74_10425) gshB 2169163..2170113 (+) 951 WP_000593273.1 glutathione synthase -
  JSQ74_RS10430 (JSQ74_10430) yqgE 2170222..2170785 (+) 564 WP_001053178.1 YqgE/AlgH family protein -
  JSQ74_RS10435 (JSQ74_10435) ruvX 2170785..2171201 (+) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  JSQ74_RS10440 (JSQ74_10440) pilT 2171377..2172357 (-) 981 WP_204084397.1 type IV pilus twitching motility protein PilT Machinery gene
  JSQ74_RS10445 (JSQ74_10445) yggS 2172375..2173079 (+) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  JSQ74_RS10450 (JSQ74_10450) yggT 2173097..2173663 (+) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  JSQ74_RS10455 (JSQ74_10455) yggU 2173660..2173950 (+) 291 WP_001277222.1 DUF167 family protein YggU -
  JSQ74_RS10460 (JSQ74_10460) rdgB 2173958..2174551 (+) 594 WP_001174735.1 XTP/dITP diphosphatase -
  JSQ74_RS10465 (JSQ74_10465) hemW 2174544..2175680 (+) 1137 WP_000239916.1 radical SAM family heme chaperone HemW -
  JSQ74_RS10470 (JSQ74_10470) yggM 2175835..2176842 (-) 1008 WP_000745210.1 DUF1202 family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35976.14 Da        Isoelectric Point: 5.7654

>NTDB_id=536535 JSQ74_RS10440 WP_204084397.1 2171377..2172357(-) (pilT) [Escherichia coli strain EA2]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLTAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGFHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=536535 JSQ74_RS10440 WP_204084397.1 2171377..2172357(-) (pilT) [Escherichia coli strain EA2]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAGTTACTCAAGAGCGAGAATGGCTTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGACGGCGATGGTTGGTTATCTCAATCAACATGCTGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATCGGTTTTCATTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter baylyi ADP1

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

46.177

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.566

100

0.457
  pilT Legionella pneumophila strain ERS1305867

44.648

100

0.448
  pilT Legionella pneumophila strain Lp02

44.648

100

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

38.906

100

0.393
  pilU Pseudomonas stutzeri DSM 10701

37.273

100

0.377
  pilU Acinetobacter baylyi ADP1

36.42

99.387

0.362
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362