Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6J77_RS04225 Genome accession   NZ_CP069535
Coordinates   976392..977429 (+) Length   345 a.a.
NCBI ID   WP_007809667.1    Uniprot ID   I4VUT4
Organism   Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 971392..982429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6J77_RS04200 (I6J77_04200) pdxH 971896..972483 (+) 588 WP_204110687.1 pyridoxamine 5'-phosphate oxidase -
  I6J77_RS04205 (I6J77_04205) - 972480..972920 (+) 441 WP_204110688.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  I6J77_RS04210 (I6J77_04210) - 972925..973734 (+) 810 WP_204110689.1 cobalamin-binding protein -
  I6J77_RS04215 (I6J77_04215) - 974613..975188 (-) 576 WP_204110690.1 YggT family protein -
  I6J77_RS04220 (I6J77_04220) proC 975185..976009 (-) 825 WP_204110691.1 pyrroline-5-carboxylate reductase -
  I6J77_RS04225 (I6J77_04225) pilT 976392..977429 (+) 1038 WP_007809667.1 type IV pilus twitching motility protein PilT Machinery gene
  I6J77_RS04230 (I6J77_04230) pilU 977584..978753 (+) 1170 WP_204110692.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6J77_RS04235 (I6J77_04235) - 978836..979204 (-) 369 WP_204110693.1 BON domain-containing protein -
  I6J77_RS04240 (I6J77_04240) - 979443..981224 (-) 1782 WP_040673521.1 hypothetical protein -
  I6J77_RS04245 (I6J77_04245) - 981309..982235 (-) 927 WP_204110694.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38531.17 Da        Isoelectric Point: 6.7246

>NTDB_id=535386 I6J77_RS04225 WP_007809667.1 976392..977429(+) (pilT) [Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPRVFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCL
QDLVKRGLVTRQEASMYAKNKDSFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=535386 I6J77_RS04225 WP_007809667.1 976392..977429(+) (pilT) [Rhodanobacter sp. FDAARGOS 1247 strain FDAARGOS_1247]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTAAAGAACAAGGCCTCGGACTTGCATCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTTGATGGCGATGTCCGCCGCATCAACATCCCTGCGCTGGAACACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAAGAGTTCTACGAGACCGACTTCTCGTTCGAGATTCCCGGGCTGGCCCGT
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCGGGCGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGGGTTGCCCCCGGGTGTTCAAGGAACTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGTCACATGCTCTCGGTCGAGGAC
CCGATCGAGTTCGTGCATACCTCGCAAAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGATACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCATTGCGCGAGGACCCGGATTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCCACCGTGCACACCAGCTCGGCGGCGAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTCATCTCGCAGTC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCGTCGCGGCGCACGAGATCATGGTCGGCATCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTG
CAGGACTTGGTCAAGCGTGGCCTGGTGACCCGTCAGGAAGCCTCGATGTACGCCAAGAACAAGGACAGCTTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4VUT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.27

96.522

0.678
  pilT Vibrio cholerae strain A1552

70.27

96.522

0.678
  pilT Neisseria meningitidis 8013

63.768

100

0.638
  pilT Neisseria gonorrhoeae MS11

63.478

100

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.547

95.942

0.475
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Vibrio cholerae strain A1552

39.017

100

0.391
  pilU Acinetobacter baylyi ADP1

38.329

100

0.386