Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6K04_RS15140 Genome accession   NZ_CP069442
Coordinates   3237512..3238549 (+) Length   345 a.a.
NCBI ID   WP_011133734.1    Uniprot ID   Q7P1N4
Organism   Chromobacterium violaceum strain FDAARGOS_1274     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3232512..3243549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K04_RS15110 (I6K04_15110) aroG 3232959..3234017 (-) 1059 WP_011133728.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  I6K04_RS15115 (I6K04_15115) dksA 3234151..3234561 (-) 411 WP_011133729.1 RNA polymerase-binding protein DksA -
  I6K04_RS15120 (I6K04_15120) - 3234845..3235150 (-) 306 WP_011133730.1 c-type cytochrome -
  I6K04_RS15125 (I6K04_15125) - 3235230..3235811 (-) 582 WP_045051079.1 YggT family protein -
  I6K04_RS15130 (I6K04_15130) proC 3235821..3236624 (-) 804 WP_081527360.1 pyrroline-5-carboxylate reductase -
  I6K04_RS15135 (I6K04_15135) - 3236679..3237374 (-) 696 WP_204086480.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6K04_RS15140 (I6K04_15140) pilT 3237512..3238549 (+) 1038 WP_011133734.1 type IV pilus twitching motility protein PilT Machinery gene
  I6K04_RS15145 (I6K04_15145) pilU 3238612..3239748 (+) 1137 WP_011133735.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6K04_RS15150 (I6K04_15150) - 3239774..3241387 (+) 1614 WP_139795212.1 response regulator -
  I6K04_RS15155 (I6K04_15155) - 3241422..3243035 (+) 1614 WP_052942147.1 response regulator -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38337.95 Da        Isoelectric Point: 6.7801

>NTDB_id=534738 I6K04_RS15140 WP_011133734.1 3237512..3238549(+) (pilT) [Chromobacterium violaceum strain FDAARGOS_1274]
MEISELLAFTVKNKASDLHLSAGLPPMIRVNGDIRRINLPPLDHHDVHDMVYDIMNDYQRKTFEDTYECDFSFELPGIAR
FRVNSFIQNRGMASVFRVIPSKVLTLEQLSAPRIFQDIANYPRGLVLVTGPTGSGKSTTLAAMIDYVNENAFSHILTVED
PIEFVHESKKSLINQRELGLQTHSFANALKSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAGEKEMVRSMLSESVRAVIAQTLLKTKDGQGRVAAHEIMIGTPAIRNLIRENKIAQINSMIQTGQQHGMQTLDQC
LADLTRRNIVSPAEARAKASHKDAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=534738 I6K04_RS15140 WP_011133734.1 3237512..3238549(+) (pilT) [Chromobacterium violaceum strain FDAARGOS_1274]
ATGGAAATTTCCGAGCTCCTGGCTTTTACCGTCAAGAACAAGGCCTCCGATCTGCACCTGTCGGCCGGCCTGCCGCCGAT
GATACGGGTCAACGGCGACATCCGCCGGATCAATCTGCCGCCGCTGGACCACCATGACGTGCACGACATGGTGTACGACA
TCATGAACGACTACCAGCGCAAGACCTTCGAAGACACCTACGAGTGCGACTTCTCGTTCGAGCTGCCCGGCATCGCCCGC
TTCCGCGTCAACTCCTTCATCCAGAACCGCGGCATGGCCTCGGTGTTCCGGGTGATTCCGAGCAAGGTGCTGACGCTGGA
GCAGCTGTCGGCGCCGCGCATCTTCCAGGACATCGCCAACTACCCGCGCGGCCTGGTGCTGGTGACCGGCCCCACCGGCT
CCGGCAAGTCGACCACGCTGGCGGCGATGATAGACTACGTCAACGAAAACGCCTTCTCGCACATTCTTACCGTGGAAGAC
CCGATCGAGTTCGTCCACGAGAGCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGCCTGCAGACCCACAGCTTCGCCAA
CGCGCTGAAAAGCGCGCTGCGCGAGGACCCGGACGTGATCCTGGTCGGCGAATTGCGCGACCTGGAAACGATACGGCTGG
CGCTGACCGCCGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCAGCAGCGCGGCCAAGACCATAGACCGGATC
GTCGACGTGTTCCCCGCCGGCGAGAAGGAAATGGTGCGCTCGATGCTGTCGGAGTCGGTGCGCGCGGTGATCGCGCAGAC
GCTGCTGAAGACCAAGGACGGCCAGGGCCGGGTGGCCGCGCACGAGATCATGATAGGCACGCCGGCCATCCGCAACCTGA
TACGCGAGAACAAGATCGCCCAGATCAACTCGATGATACAGACCGGGCAGCAACACGGCATGCAGACGCTGGACCAGTGC
CTGGCCGACCTGACCCGGCGCAACATCGTGTCGCCGGCCGAGGCGCGCGCCAAGGCCAGCCACAAGGACGCATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7P1N4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

72.754

100

0.728
  pilT Acinetobacter nosocomialis M2

72.754

100

0.728
  pilT Acinetobacter baumannii strain A118

72.754

100

0.728
  pilT Acinetobacter baylyi ADP1

71.014

100

0.71
  pilT Pseudomonas aeruginosa PAK

71.014

100

0.71
  pilT Pseudomonas stutzeri DSM 10701

71.014

100

0.71
  pilT Neisseria meningitidis 8013

70.435

100

0.704
  pilT Neisseria gonorrhoeae MS11

70.435

100

0.704
  pilT Legionella pneumophila strain Lp02

68.986

100

0.69
  pilT Legionella pneumophila strain ERS1305867

68.986

100

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.456

97.971

0.661
  pilT Vibrio cholerae strain A1552

67.456

97.971

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.474

95.942

0.513
  pilU Vibrio cholerae strain A1552

41.493

97.101

0.403
  pilU Acinetobacter baylyi ADP1

41.369

97.391

0.403
  pilU Pseudomonas stutzeri DSM 10701

42.901

93.913

0.403