Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MASE_RS14370 Genome accession   NC_018632
Coordinates   3359520..3360563 (-) Length   347 a.a.
NCBI ID   WP_014950464.1    Uniprot ID   -
Organism   Alteromonas macleodii ATCC 27126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3354520..3365563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MASE_RS14340 (MASE_14385) - 3354955..3355683 (+) 729 WP_014950458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MASE_RS14345 (MASE_14390) gshB 3355822..3356775 (+) 954 WP_014950459.1 glutathione synthase -
  MASE_RS14350 (MASE_14395) - 3356904..3357470 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  MASE_RS14355 (MASE_14400) ruvX 3357463..3357894 (+) 432 WP_014950461.1 Holliday junction resolvase RuvX -
  MASE_RS14360 (MASE_14405) - 3357941..3358258 (-) 318 WP_014950462.1 DUF2007 domain-containing protein -
  MASE_RS14365 (MASE_14410) pilU 3358399..3359511 (-) 1113 WP_014950463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MASE_RS14370 (MASE_14415) pilT 3359520..3360563 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  MASE_RS14375 (MASE_14420) - 3360591..3361277 (+) 687 WP_014950465.1 YggS family pyridoxal phosphate-dependent enzyme -
  MASE_RS14380 (MASE_14425) proC 3361348..3362169 (+) 822 WP_014950466.1 pyrroline-5-carboxylate reductase -
  MASE_RS14385 (MASE_14430) - 3362190..3362726 (+) 537 WP_014950467.1 YggT family protein -
  MASE_RS14390 (MASE_14435) - 3362823..3363296 (+) 474 WP_014950468.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38380.09 Da        Isoelectric Point: 6.5677

>NTDB_id=53428 MASE_RS14370 WP_014950464.1 3359520..3360563(-) (pilT) [Alteromonas macleodii ATCC 27126]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHGLVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAAKSVDKQSMNSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=53428 MASE_RS14370 WP_014950464.1 3359520..3360563(-) (pilT) [Alteromonas macleodii ATCC 27126]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCGGGCCTACCACCAAT
AATTCGTGTAGATGGCGAAATGCGTAAACTGAACGTGCCAGCCCTTGATCACAAGCAAGTACATGGTCTCGTTTATGAGA
TTATGAATGACATGCAGCGCAAAGAATACGAAGAAAACCTGGAAACCGATTTCTCTTTCGAAGTGAATGGCTTGTCGCGC
TTTCGTGTTAACGCGTTTGTGCAAAACCGAGGCGCCGCGGCAGTATTACGTACTATCCCCAGTAAAGTATTAACCTTAGA
CGACCTTGGCGCGCCTGAAATTTTCAAGGAAATTATAAATCAGCCTACCGGCATTGTACTTGTTACAGGTGCCACCGGCT
CAGGTAAAAGTACCACGCTTGCGGCCATGGTTGACCATATCAACACCCATAAGCGCGAGCACATTCTTACCATTGAAGAC
CCTATCGAATTCGTGCACGACAACAAGCTTAGCTTGGTAAACCAGCGGGAAGTGCACCGTGATACGCACTCGTTTAATAA
CGCACTGCGCTCGGCGCTTCGTGAAGACCCAGACGTAATTCTAGTCGGTGAGCTACGTGACTTAGAAACCATACGCCTTG
CCATTTCCGCTGCCGAAACCGGCCATTTAGTATTCGGCACTCTTCACACCAACTCTGCACCAAAAACCATTGACCGTATT
ATCGATGTTTTCCCGGCAGAAGAAAAAGCCATGGTGCGTTCTATGTTGTCGGAATCATTGCGTGCGGTTATTTCACAGAC
ACTGCTTAAGAAAGTAGGCGGGGGCCGAGTTGCGGCTCATGAAATCATGGTGGGGATCCCCGCTATTCGTAACCTTATTC
GAGAAGATAAAGTGCCGCAAATGTACTCGGTTATTCAAACGGGCCAGGCTCACGGTATGCAAACTATGGATCAGTGTCTT
CAGCGCTTAGTGGCGATGGGGGTTATCACCAAACAAGACGCCGCAGCAAAGTCAGTTGATAAACAGTCGATGAACAGTTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter nosocomialis M2

75.581

99.135

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

74.852

97.406

0.729
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

65.029

99.712

0.648
  pilT Neisseria gonorrhoeae MS11

64.74

99.712

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

98.847

0.513
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

41.42

97.406

0.403

Multiple sequence alignment