Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MASE_RS14365 Genome accession   NC_018632
Coordinates   3358399..3359511 (-) Length   370 a.a.
NCBI ID   WP_014950463.1    Uniprot ID   -
Organism   Alteromonas macleodii ATCC 27126     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3353399..3364511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MASE_RS14340 (MASE_14385) - 3354955..3355683 (+) 729 WP_014950458.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MASE_RS14345 (MASE_14390) gshB 3355822..3356775 (+) 954 WP_014950459.1 glutathione synthase -
  MASE_RS14350 (MASE_14395) - 3356904..3357470 (+) 567 WP_014950460.1 YqgE/AlgH family protein -
  MASE_RS14355 (MASE_14400) ruvX 3357463..3357894 (+) 432 WP_014950461.1 Holliday junction resolvase RuvX -
  MASE_RS14360 (MASE_14405) - 3357941..3358258 (-) 318 WP_014950462.1 DUF2007 domain-containing protein -
  MASE_RS14365 (MASE_14410) pilU 3358399..3359511 (-) 1113 WP_014950463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MASE_RS14370 (MASE_14415) pilT 3359520..3360563 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  MASE_RS14375 (MASE_14420) - 3360591..3361277 (+) 687 WP_014950465.1 YggS family pyridoxal phosphate-dependent enzyme -
  MASE_RS14380 (MASE_14425) proC 3361348..3362169 (+) 822 WP_014950466.1 pyrroline-5-carboxylate reductase -
  MASE_RS14385 (MASE_14430) - 3362190..3362726 (+) 537 WP_014950467.1 YggT family protein -
  MASE_RS14390 (MASE_14435) - 3362823..3363296 (+) 474 WP_014950468.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40780.71 Da        Isoelectric Point: 6.1868

>NTDB_id=53427 MASE_RS14365 WP_014950463.1 3358399..3359511(-) (pilU) [Alteromonas macleodii ATCC 27126]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTESATSEEASLALVHEAMTEKQQNEFHTTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFNDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=53427 MASE_RS14365 WP_014950463.1 3358399..3359511(-) (pilU) [Alteromonas macleodii ATCC 27126]
ATGTTAGATTCACTACTACAAAAAATGGTCGATACCAACGCATCGGATTTATTCGTAACGGCGGGGTTTCCGGTAAGTGC
CAAAATTAACGGCAAGCTTACGCCAGTGACGGAAAGCGCGACCTCGGAAGAGGCATCGCTGGCGCTGGTTCACGAAGCGA
TGACTGAAAAACAGCAGAATGAGTTTCACACGACCAAAGAATGTAATTTCGCCATAGTACGAGAGGGCATTGGTCGTTTC
CGCTGCTCTGCATTTTGGCAGCGTGACCAAGCGGGCATGGTGATTCGCCGCATCGTTACTCAAATTCCGAAAGCTGATGA
TTTGGGTCTGCCGCGAGTATTAAAAGAAATTATTATGGCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGCACGG
GTAAGTCCACGTCATTGGCTGCGCTTATTGGGCATAGAAATGAAAATTCGCACGGGCATATACTGACTATTGAAGACCCC
ATTGAATTTGTGCACGAGCATAAAAACTGTGTTATTACCCAGCGCGAGGTGGGCATAGATACTCAGTCTTTCAACGACGC
CCTTAAAAGTTCGCTACGTCAGGCACCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAAACCATGGAATATGCCA
TGTCGTTTGCTGATACAGGGCATTTGTGTGTGGCTACGCTGCACGCTAACAACGCGAACCAAGCTATTGAACGTATTATG
CATTTAGCGCCGAAAGATCAGCACGATAAGTTGCGTTTTGACTTAAGCTTAAACATCCGCGCCATTGTTGCGCAGCAACT
AGTACCTACGCCAGACGGCAAGGGTAGGGTTGCGGCAATAGAAATATTGCTAAACTCGCCGCTAGTGCGCGACCTAATAC
AGCGCAATGAAATAGGCGCGTTAAAAGAAGCAATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCGCTG
TATGATTTGTACAAAGCGGGTAAGATTGATATGGACCAAGCGCTGCACCACGCCGACTCGCCTAATGACCTGCGCTTAAT
GATCAAGCTTGATACCAGCGACGGTTCTAGCTTAGGTACACTGTCAAATGTATCTATTGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilU Vibrio cholerae strain A1552

56.25

99.459

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.353

91.892

0.389
  pilT Acinetobacter nosocomialis M2

39.706

91.892

0.365
  pilT Acinetobacter baumannii D1279779

39.706

91.892

0.365
  pilT Acinetobacter baumannii strain A118

39.706

91.892

0.365

Multiple sequence alignment