Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JNO50_RS00520 Genome accession   NZ_CP069161
Coordinates   124999..126036 (+) Length   345 a.a.
NCBI ID   WP_189532734.1    Uniprot ID   A0A918P2I2
Organism   Paludibacterium paludis strain BCRC 80514     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 119999..131036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO50_RS00490 aroG 120570..121634 (-) 1065 WP_189532717.1 3-deoxy-7-phosphoheptulonate synthase AroG -
  JNO50_RS00495 dksA 121782..122192 (-) 411 WP_189532724.1 RNA polymerase-binding protein DksA -
  JNO50_RS00500 - 122379..122684 (-) 306 WP_189532726.1 c-type cytochrome -
  JNO50_RS00505 - 122770..123351 (-) 582 WP_189532728.1 YggT family protein -
  JNO50_RS00510 proC 123353..124156 (-) 804 WP_189532729.1 pyrroline-5-carboxylate reductase -
  JNO50_RS00515 - 124170..124865 (-) 696 WP_189532731.1 YggS family pyridoxal phosphate-dependent enzyme -
  JNO50_RS00520 pilT 124999..126036 (+) 1038 WP_189532734.1 type IV pilus twitching motility protein PilT Machinery gene
  JNO50_RS00525 pilU 126055..127191 (+) 1137 WP_189532736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JNO50_RS00530 - 127270..127998 (-) 729 WP_189532910.1 amino acid ABC transporter ATP-binding protein -
  JNO50_RS00535 - 128004..128678 (-) 675 WP_189532738.1 ABC transporter permease subunit -
  JNO50_RS00540 - 128675..129421 (-) 747 WP_189532740.1 amino acid ABC transporter permease -
  JNO50_RS00545 - 129485..130378 (-) 894 WP_229804572.1 glutamate/aspartate ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38143.93 Da        Isoelectric Point: 6.5058

>NTDB_id=532541 JNO50_RS00520 WP_189532734.1 124999..126036(+) (pilT) [Paludibacterium paludis strain BCRC 80514]
MEISELLAFTVKNQASDLHLSSGMPPLLRVNGDIRRVNLPPMTSHDVHSMIYDIMNDYQRKGFEETLESDFSFALPGIAR
FRVNVFMQNRGIAAVFRVIPSHVFTLDELDGPRILKELCDLPRGLVLVTGPTGSGKSTTLAAMIDFINATRFAHILTIED
PIEFVHDSKQSLINQRELGQQTMSFANALRSALREDPDVIMVGELRDLETIRLALTAAETGHLVFASLHTSSAPKTIDRI
VDVFPAGEKDMVRTMLSESLRAVVSQTLIKQSDGNGRVAAFEIMMGTPAVRNLIRENKLAQLLTAMQTGSQSGMQTLDQA
LQELLRRGRISLADARGKAAQKDLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=532541 JNO50_RS00520 WP_189532734.1 124999..126036(+) (pilT) [Paludibacterium paludis strain BCRC 80514]
ATGGAAATTTCCGAGCTGTTGGCGTTCACGGTCAAAAACCAGGCTTCCGACCTTCACCTGTCGTCGGGCATGCCCCCGCT
GCTCAGGGTCAATGGCGACATCCGCCGCGTCAACCTGCCGCCGATGACGTCCCATGATGTCCACTCGATGATCTACGACA
TCATGAACGATTATCAGCGCAAAGGCTTCGAAGAAACCCTCGAGAGCGACTTCTCGTTCGCGCTGCCGGGCATCGCGCGC
TTCCGGGTCAACGTATTCATGCAAAATCGCGGTATCGCCGCCGTGTTCCGCGTGATTCCCTCTCATGTGTTCACCCTCGA
CGAACTCGACGGCCCGCGCATCCTGAAGGAACTGTGCGATCTGCCGCGCGGCCTCGTACTGGTCACCGGCCCGACCGGTT
CGGGCAAATCCACCACGCTCGCCGCGATGATCGATTTCATCAACGCCACCCGTTTCGCGCACATCCTCACGATCGAGGAC
CCGATCGAATTCGTGCACGACAGCAAGCAAAGCCTCATCAACCAGCGCGAACTCGGGCAACAGACCATGAGTTTCGCCAA
CGCCCTGCGCAGCGCGTTGCGCGAGGATCCGGACGTGATCATGGTCGGCGAGCTGCGGGACCTGGAAACCATCCGGCTCG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCCTCGCTGCACACCAGTTCCGCCCCGAAAACCATCGACCGGATC
GTCGATGTGTTTCCGGCGGGAGAAAAAGACATGGTGCGCACCATGCTGTCCGAATCGTTGCGCGCGGTGGTGTCGCAGAC
CCTGATCAAGCAAAGCGACGGCAACGGACGGGTCGCGGCGTTCGAAATCATGATGGGCACACCGGCCGTGCGCAACCTCA
TCCGGGAAAACAAGCTGGCCCAGTTGCTGACGGCCATGCAGACAGGCAGCCAGTCGGGCATGCAGACGCTCGACCAGGCA
TTGCAGGAACTGTTGCGCCGCGGCCGCATCTCGCTGGCCGACGCGCGTGGCAAGGCCGCCCAGAAAGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

67.536

100

0.675
  pilT Acinetobacter baumannii D1279779

67.536

100

0.675
  pilT Acinetobacter baumannii strain A118

67.536

100

0.675
  pilT Acinetobacter baylyi ADP1

67.536

100

0.675
  pilT Pseudomonas aeruginosa PAK

68.437

98.261

0.672
  pilT Pseudomonas stutzeri DSM 10701

67.246

100

0.672
  pilT Neisseria meningitidis 8013

66.764

99.42

0.664
  pilT Neisseria gonorrhoeae MS11

66.472

99.42

0.661
  pilT Legionella pneumophila strain Lp02

65.217

100

0.652
  pilT Legionella pneumophila strain ERS1305867

65.217

100

0.652
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

61.834

97.971

0.606
  pilT Vibrio cholerae strain A1552

61.834

97.971

0.606
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.905

94.783

0.501
  pilU Pseudomonas stutzeri DSM 10701

42.687

97.101

0.414
  pilU Acinetobacter baylyi ADP1

41.369

97.391

0.403
  pilU Vibrio cholerae strain A1552

40.299

97.101

0.391