Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JNO50_RS00525 Genome accession   NZ_CP069161
Coordinates   126055..127191 (+) Length   378 a.a.
NCBI ID   WP_189532736.1    Uniprot ID   A0A918P1B9
Organism   Paludibacterium paludis strain BCRC 80514     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121055..132191
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JNO50_RS00495 dksA 121782..122192 (-) 411 WP_189532724.1 RNA polymerase-binding protein DksA -
  JNO50_RS00500 - 122379..122684 (-) 306 WP_189532726.1 c-type cytochrome -
  JNO50_RS00505 - 122770..123351 (-) 582 WP_189532728.1 YggT family protein -
  JNO50_RS00510 proC 123353..124156 (-) 804 WP_189532729.1 pyrroline-5-carboxylate reductase -
  JNO50_RS00515 - 124170..124865 (-) 696 WP_189532731.1 YggS family pyridoxal phosphate-dependent enzyme -
  JNO50_RS00520 pilT 124999..126036 (+) 1038 WP_189532734.1 type IV pilus twitching motility protein PilT Machinery gene
  JNO50_RS00525 pilU 126055..127191 (+) 1137 WP_189532736.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JNO50_RS00530 - 127270..127998 (-) 729 WP_189532910.1 amino acid ABC transporter ATP-binding protein -
  JNO50_RS00535 - 128004..128678 (-) 675 WP_189532738.1 ABC transporter permease subunit -
  JNO50_RS00540 - 128675..129421 (-) 747 WP_189532740.1 amino acid ABC transporter permease -
  JNO50_RS00545 - 129485..130378 (-) 894 WP_229804572.1 glutamate/aspartate ABC transporter substrate-binding protein -
  JNO50_RS00550 - 130545..131279 (-) 735 WP_189532744.1 amino acid ABC transporter ATP-binding protein -
  JNO50_RS00555 rsmA 131393..132175 (-) 783 WP_189532746.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42014.37 Da        Isoelectric Point: 6.5056

>NTDB_id=532542 JNO50_RS00525 WP_189532736.1 126055..127191(+) (pilU) [Paludibacterium paludis strain BCRC 80514]
MDQHEARRLIADLLATAVARHASDVFVSADFPPALKIDGRLQPAMEKPLLAQEAKELVQAMMTAHHLGEFTATKEANFAI
TQEGIGRFRVSAFMQQGHPAMVIRRITTDIPTLDELRLPAVLKDISMIRRGLVIFVGGTGSGKSTSLAALIDWRNTHAED
HIITIEEPVEFIHHHKKSLITQREVGIDTDSWGIALKNTLRQAPDVIMMGEVRDQASMMYALQFAETGHLCLATLHANNA
NQALERVINFFPEERRQQVLLDLSLNMRAIISQRLVPLKGGKGRCAAVEVLLNSPLVSDLIVSGDVHGIKDIMGRSTDLG
MRTFDQSLFELYEEGRVGLEDALRNADSINDLRLRIKLYSERSKSRDPLGGLDHLDLV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=532542 JNO50_RS00525 WP_189532736.1 126055..127191(+) (pilU) [Paludibacterium paludis strain BCRC 80514]
ATGGATCAACACGAGGCTCGGCGGCTGATCGCCGATCTTCTGGCAACGGCGGTCGCCCGTCACGCCTCCGATGTGTTCGT
CAGCGCGGATTTTCCCCCCGCCCTGAAAATCGACGGACGCCTGCAACCCGCGATGGAAAAACCCCTGCTCGCGCAGGAAG
CGAAAGAGCTGGTGCAGGCGATGATGACGGCGCATCACCTTGGCGAATTCACCGCCACCAAGGAGGCGAACTTCGCCATC
ACCCAGGAGGGCATCGGCCGCTTCCGGGTCAGCGCCTTCATGCAACAAGGACACCCGGCGATGGTGATCCGCCGCATCAC
CACCGACATCCCGACGCTTGACGAGTTGCGCCTGCCCGCCGTGCTCAAGGATATTTCGATGATCCGGCGCGGGCTGGTGA
TTTTCGTGGGAGGCACCGGGTCGGGCAAATCCACCTCGCTCGCCGCGCTGATCGACTGGCGCAATACCCATGCCGAAGAC
CATATCATCACCATCGAGGAACCGGTCGAATTCATCCATCATCACAAAAAGAGCCTGATCACGCAGCGGGAGGTCGGCAT
CGATACCGACAGCTGGGGAATCGCGCTGAAGAACACGCTACGCCAGGCTCCCGACGTGATCATGATGGGCGAGGTCCGCG
ATCAGGCCTCGATGATGTATGCGCTGCAATTCGCCGAAACCGGCCACTTGTGCCTGGCCACCCTGCACGCCAACAACGCC
AACCAGGCGCTGGAACGGGTCATCAACTTCTTCCCCGAGGAGCGCCGCCAACAGGTGCTGCTCGACCTGTCCCTCAACAT
GCGCGCCATCATTTCGCAACGGCTGGTGCCGCTCAAGGGCGGCAAGGGCCGTTGCGCGGCGGTGGAAGTCCTGCTCAACA
GCCCGCTGGTGTCGGATCTGATCGTCTCGGGCGATGTGCACGGCATCAAGGACATCATGGGCCGCTCCACCGACCTTGGC
ATGCGCACCTTCGACCAGTCCTTGTTCGAACTGTACGAGGAGGGCCGCGTCGGCCTCGAGGACGCGCTGCGCAACGCCGA
CTCCATCAACGACCTGCGCTTGCGCATCAAGCTCTATAGCGAGCGAAGCAAATCGCGCGATCCGCTGGGAGGACTCGATC
ATCTGGATCTGGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(15-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.166

92.328

0.537
  pilU Acinetobacter baylyi ADP1

55.462

94.444

0.524
  pilU Vibrio cholerae strain A1552

52.679

88.889

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.95

89.153

0.365