Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JK628_RS06680 Genome accession   NZ_CP068437
Coordinates   1520302..1521339 (+) Length   345 a.a.
NCBI ID   WP_202288687.1    Uniprot ID   -
Organism   Shewanella sp. KX20019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1515302..1526339
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JK628_RS06650 (JK628_06650) rdgB 1516381..1516980 (-) 600 WP_202288680.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JK628_RS06655 (JK628_06655) - 1517200..1517634 (-) 435 WP_202288681.1 DUF4426 domain-containing protein -
  JK628_RS06660 (JK628_06660) yggU 1517718..1518005 (-) 288 WP_202288682.1 DUF167 family protein YggU -
  JK628_RS06665 (JK628_06665) - 1518005..1518553 (-) 549 WP_202288683.1 YggT family protein -
  JK628_RS06670 (JK628_06670) proC 1518589..1519407 (-) 819 WP_202288684.1 pyrroline-5-carboxylate reductase -
  JK628_RS06675 (JK628_06675) - 1519571..1520266 (-) 696 WP_202288686.1 YggS family pyridoxal phosphate-dependent enzyme -
  JK628_RS06680 (JK628_06680) pilT 1520302..1521339 (+) 1038 WP_202288687.1 type IV pilus twitching motility protein PilT Machinery gene
  JK628_RS06685 (JK628_06685) pilU 1521354..1522466 (+) 1113 WP_202288688.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JK628_RS06690 (JK628_06690) hemH 1522673..1523686 (+) 1014 WP_202288689.1 ferrochelatase -
  JK628_RS06695 (JK628_06695) - 1523772..1524161 (-) 390 WP_202288690.1 DUF3192 domain-containing protein -
  JK628_RS06700 (JK628_06700) xni 1524273..1525037 (-) 765 WP_202288691.1 flap endonuclease Xni -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38314.84 Da        Isoelectric Point: 6.7842

>NTDB_id=529060 JK628_RS06680 WP_202288687.1 1520302..1521339(+) (pilT) [Shewanella sp. KX20019]
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKIASFPRGLVLVTGPTGSGKSTTLAAMVDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQITREDAMLKSSNKQTNF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=529060 JK628_RS06680 WP_202288687.1 1520302..1521339(+) (pilT) [Shewanella sp. KX20019]
ATGGAAATCACAGAATTACTTGCCTTTAGTGTAAAGCACAATGCATCAGATCTACACCTTTCTGCGGGGGTTTCTCCTAT
GATCCGTGTTGATGGTGAAGTTAGGAAGATCAACCTTCCAGCTTTAGACCATCAAGGTGTACATAGTCTTGTGTACGATA
TAATGAATGATAAGCAGCGAAAAGATTATGAAGAGCATCTTGAGATCGATTTCTCGTTTGAAGTGCCGGGTCTTGCCCGC
TTTCGTGTTAATGCGTTTAACCAATCCCGTGGTTCAGCAGCGGTATTTCGTACCATCCCCAGTGATATCTTGAGCCTTGA
GCAACTTGGCGCGCCAGAAATCTTTAAAAAAATAGCTAGTTTCCCGCGAGGACTGGTATTGGTCACTGGCCCGACTGGCT
CAGGTAAGAGTACGACGCTAGCTGCGATGGTTGATTATGTGAATGAAAATCGCCATGACCACATATTGACGATTGAAGAC
CCAATAGAATTTGTACATCAAAACAAGCAATGCCTGATTAACCAACGTGAAGTTCATCGCCATACTCATAGTTTTAATGC
CGCACTACGCAGTGCGCTACGTGAAGACCCCGATGTTATTCTTGTCGGTGAGATGCGCGATCTTGAAACGATCAGATTGG
CGATGACGGCTGCTGAAACGGGTCATCTTGTGTTTGGTACCTTGCATACAACTTCTGCGGCTAAAACGATTGACCGTGTG
GTTGATGTGTTCCCCGAGGGGGAAAAAGGCATGGTGCGGACAATGTTATCTGAATCACTGCAAGCCGTTATTTCTCAGAC
CTTGATTAAAAAGGTCGGTGGTGGTCGTGTGGCTGCTCATGAGATTATGATGGGGACGCCAGCGATCCGTAACTTGATCC
GCGAAGATAAAGTCGCGCAAATGTACTCCGCAATTCAAACGGGTATGGCGCACGGCATGCAAACACTGGATCAATGTCTG
CAGAACTTGGTTAATCGCGGTCAAATTACCCGTGAAGACGCCATGCTTAAAAGCTCAAATAAGCAAACCAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.532

99.13

0.788
  pilT Acinetobacter baumannii D1279779

79.532

99.13

0.788
  pilT Acinetobacter baumannii strain A118

79.532

99.13

0.788
  pilT Pseudomonas stutzeri DSM 10701

77.101

100

0.771
  pilT Pseudomonas aeruginosa PAK

78.402

97.971

0.768
  pilT Acinetobacter baylyi ADP1

79.331

95.362

0.757
  pilT Legionella pneumophila strain Lp02

74.194

98.841

0.733
  pilT Legionella pneumophila strain ERS1305867

74.194

98.841

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.997

97.681

0.713
  pilT Vibrio cholerae strain A1552

72.997

97.681

0.713
  pilT Neisseria meningitidis 8013

68.605

99.71

0.684
  pilT Neisseria gonorrhoeae MS11

68.314

99.71

0.681
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.853

98.261

0.49
  pilU Pseudomonas stutzeri DSM 10701

42.274

99.42

0.42
  pilU Vibrio cholerae strain A1552

41.369

97.391

0.403
  pilU Acinetobacter baylyi ADP1

39.481

100

0.397