Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JK628_RS06685 Genome accession   NZ_CP068437
Coordinates   1521354..1522466 (+) Length   370 a.a.
NCBI ID   WP_202288688.1    Uniprot ID   -
Organism   Shewanella sp. KX20019     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1516354..1527466
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JK628_RS06650 (JK628_06650) rdgB 1516381..1516980 (-) 600 WP_202288680.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  JK628_RS06655 (JK628_06655) - 1517200..1517634 (-) 435 WP_202288681.1 DUF4426 domain-containing protein -
  JK628_RS06660 (JK628_06660) yggU 1517718..1518005 (-) 288 WP_202288682.1 DUF167 family protein YggU -
  JK628_RS06665 (JK628_06665) - 1518005..1518553 (-) 549 WP_202288683.1 YggT family protein -
  JK628_RS06670 (JK628_06670) proC 1518589..1519407 (-) 819 WP_202288684.1 pyrroline-5-carboxylate reductase -
  JK628_RS06675 (JK628_06675) - 1519571..1520266 (-) 696 WP_202288686.1 YggS family pyridoxal phosphate-dependent enzyme -
  JK628_RS06680 (JK628_06680) pilT 1520302..1521339 (+) 1038 WP_202288687.1 type IV pilus twitching motility protein PilT Machinery gene
  JK628_RS06685 (JK628_06685) pilU 1521354..1522466 (+) 1113 WP_202288688.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JK628_RS06690 (JK628_06690) hemH 1522673..1523686 (+) 1014 WP_202288689.1 ferrochelatase -
  JK628_RS06695 (JK628_06695) - 1523772..1524161 (-) 390 WP_202288690.1 DUF3192 domain-containing protein -
  JK628_RS06700 (JK628_06700) xni 1524273..1525037 (-) 765 WP_202288691.1 flap endonuclease Xni -
  JK628_RS06705 (JK628_06705) ppnN 1525034..1526386 (-) 1353 WP_202288692.1 nucleotide 5'-monophosphate nucleosidase PpnN -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40895.68 Da        Isoelectric Point: 6.1879

>NTDB_id=529061 JK628_RS06685 WP_202288688.1 1521354..1522466(+) (pilU) [Shewanella sp. KX20019]
MDVRPFLKAMTDRKASDLFITAGFPPSAKIDGELRPLSESTFSPAQALDFVESLMTDVQKEEFHQSRECNFAFAAKDLGR
FRVSAFWQREAPGCVMRRIETKIPDVDELMLPPILKDLVMSKRGLVIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGVDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHSLKETMSKSNEQGMQTFDQA
LLRLYADGEISYADALHHADSPNDLRLMIKLQSSDTTSSSSMDGVTLNLD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=529061 JK628_RS06685 WP_202288688.1 1521354..1522466(+) (pilU) [Shewanella sp. KX20019]
ATGGATGTTCGTCCCTTTTTAAAGGCTATGACAGATCGTAAAGCCTCAGACCTCTTCATTACTGCAGGCTTTCCACCTAG
TGCAAAAATTGATGGTGAGTTACGGCCGTTGAGTGAGAGCACATTTAGTCCCGCGCAGGCATTAGATTTTGTTGAATCAT
TAATGACAGATGTACAAAAAGAGGAGTTTCACCAATCTCGCGAATGTAACTTCGCTTTTGCCGCTAAAGATCTTGGCCGA
TTTCGTGTTAGTGCTTTTTGGCAAAGAGAAGCTCCAGGCTGCGTAATGCGTCGTATTGAGACGAAAATCCCAGATGTCGA
TGAGTTAATGTTGCCGCCTATTCTAAAAGACTTAGTGATGAGTAAGCGTGGCTTAGTGATCATGGTTGGCGGTACGGGTA
CCGGTAAATCTACCTCATTAGCGGCACTCGTTGGTTATCGAAACGCACATGCACGCGGGCATATCTTAACCATTGAAGAT
CCGGTTGAATTTGTTCACGATCACCGCAAAAGTATTATCACTCAGCGTGAAGTGGGCGTCGATACTGAATCTTTTGATGC
GGCGCTTAAAAGCTCACTGCGCCAGGCACCAGATGTTATTTTGATTGGTGAGATCCGTAGCCAAGAGACCATGGAGTTCG
CATTGTCTTTTGCCGAAACCGGTCATCTTTGTATGGCAACATTGCATGCTAACAATGCTAACCAAGCACTTGATCGTATT
ATGCATTTGGTACCAGAAAGTAAACATCATCAATTGTTATTTGATCTGTCGCTAAATTTACGAGGCATTGTAGCCCAGCA
GCTGGTGCCTAAAAGCGACGGTTCTGGCCGAAGAGCCGCAATTGAAGTGTTGATTAATACGCCTCGAGTGGCCAGTTTGA
TCGCTAAAAATGAATTGCACTCGTTAAAGGAGACCATGTCCAAGTCTAATGAGCAGGGCATGCAGACGTTCGATCAGGCA
TTGTTAAGGCTCTATGCTGATGGTGAAATTAGCTACGCAGATGCACTGCACCATGCCGATTCACCGAATGACTTACGATT
AATGATTAAGTTGCAAAGTTCAGATACAACGAGTTCAAGTTCTATGGATGGGGTAACACTCAACTTGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.76

100

0.589
  pilU Vibrio cholerae strain A1552

58.649

100

0.586
  pilU Acinetobacter baylyi ADP1

59.659

95.135

0.568
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.739

93.243

0.389
  pilT Vibrio cholerae strain A1552

42.424

89.189

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.424

89.189

0.378
  pilT Acinetobacter baylyi ADP1

39.181

92.432

0.362
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Legionella pneumophila strain Lp02

40.483

89.459

0.362
  pilT Legionella pneumophila strain ERS1305867

40.483

89.459

0.362