Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JKJ11_RS14990 Genome accession   NZ_CP068024
Coordinates   3207642..3208682 (+) Length   346 a.a.
NCBI ID   WP_252038401.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43133     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3202642..3213682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKJ11_RS14955 (JKJ11_14945) hemW 3202887..3204041 (-) 1155 WP_252039128.1 radical SAM family heme chaperone HemW -
  JKJ11_RS14960 (JKJ11_14950) - 3204058..3204660 (-) 603 WP_252038398.1 XTP/dITP diphosphatase -
  JKJ11_RS14965 (JKJ11_14955) - 3204709..3205140 (-) 432 WP_062459978.1 DUF4426 domain-containing protein -
  JKJ11_RS14970 (JKJ11_14960) yggU 3205165..3205452 (-) 288 WP_006075534.1 DUF167 family protein YggU -
  JKJ11_RS14975 (JKJ11_14965) - 3205452..3206009 (-) 558 WP_006075533.1 YggT family protein -
  JKJ11_RS14980 (JKJ11_14970) proC 3206041..3206859 (-) 819 WP_006075532.1 pyrroline-5-carboxylate reductase -
  JKJ11_RS14985 (JKJ11_14975) - 3206906..3207616 (-) 711 WP_252038400.1 YggS family pyridoxal phosphate-dependent enzyme -
  JKJ11_RS14990 (JKJ11_14980) pilT 3207642..3208682 (+) 1041 WP_252038401.1 type IV pilus twitching motility protein PilT Machinery gene
  JKJ11_RS14995 (JKJ11_14985) pilU 3208692..3209801 (+) 1110 WP_252038403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JKJ11_RS15025 (JKJ11_15015) - 3210679..3210819 (-) 141 WP_252038404.1 hypothetical protein -
  JKJ11_RS26105 (JKJ11_15020) - 3210940..3211119 (-) 180 WP_309245327.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38462.96 Da        Isoelectric Point: 6.2984

>NTDB_id=526344 JKJ11_RS14990 WP_252038401.1 3207642..3208682(+) (pilT) [Vibrio sp. SCSIO 43133]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGIPALEHSEVHKLVFDIMNDKQRSEYEEHLEIDFSFDVPDIGR
FRVNAFNQSRGCSAVFRTIPSEVPTLAQIDAPDKFSEIAGLERGLVLVTGPTGSGKSTTLAAMVDHINQHQNKHILTIED
PIEFVHQNKKCLVNQREVHRDTHSFKAALRSALREDPDIILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGQDKEMVRSMLSESLRAVIAQTLLKRVGGGRIACHEIMLATPAIRNLIREDKVAQMYSIIQTGAASGMQTMEQHS
KRLLSQGLVELEEVNKKIEQHGSMFQ

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=526344 JKJ11_RS14990 WP_252038401.1 3207642..3208682(+) (pilT) [Vibrio sp. SCSIO 43133]
ATGGATATCACCGAGTTACTAGACTTTAGTGTAAAGCATAACGCTTCAGATCTACACCTTTCTGCTGGTGTCCCACCTAT
GGTACGTATTGATGGTGAAGTAAGAAAGCTTGGTATCCCTGCTCTAGAGCATTCTGAGGTCCATAAATTAGTTTTTGACA
TTATGAATGATAAACAGCGCAGCGAATACGAAGAGCATCTCGAAATCGATTTTTCATTTGATGTGCCCGATATCGGTCGT
TTTCGTGTCAATGCGTTTAATCAATCTCGTGGCTGCTCTGCCGTTTTTCGTACCATCCCCAGTGAAGTCCCAACACTGGC
GCAAATAGACGCACCCGACAAATTTTCCGAGATAGCCGGTTTAGAACGCGGCTTGGTATTAGTAACAGGGCCGACTGGTT
CAGGTAAATCGACGACACTAGCAGCCATGGTCGACCACATTAACCAACATCAAAACAAACATATTCTGACCATTGAGGAT
CCTATTGAGTTTGTTCATCAGAATAAAAAATGTTTGGTTAACCAGCGAGAAGTTCATCGCGACACCCACAGTTTTAAAGC
TGCACTCCGCTCTGCACTGCGTGAAGACCCGGACATCATTTTAGTTGGTGAGCTGCGAGATCAAGAGACCATTAGCTTAG
CTCTTACCGCAGCCGAAACCGGCCACTTAGTGTTTGGCACTCTGCACACGAGCTCTGCAGCCAAAACCATTGACCGAATT
ATTGATGTATTTCCAGGCCAAGATAAAGAAATGGTTCGCTCTATGCTCTCAGAATCGTTGCGAGCAGTTATCGCACAAAC
TCTGTTAAAGCGCGTGGGAGGAGGGCGCATTGCTTGTCATGAAATTATGCTAGCGACACCCGCTATCAGAAACCTAATTC
GAGAAGATAAAGTAGCTCAGATGTACTCAATTATCCAAACAGGTGCGGCAAGTGGTATGCAGACCATGGAACAGCACTCT
AAACGGTTATTGTCTCAAGGATTGGTTGAACTAGAAGAAGTGAATAAGAAGATTGAACAACATGGTTCAATGTTTCAATA
G

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

83.481

97.977

0.818
  pilT Vibrio cholerae strain A1552

83.481

97.977

0.818
  pilT Acinetobacter baylyi ADP1

72.508

95.665

0.694
  pilT Acinetobacter nosocomialis M2

72.948

95.087

0.694
  pilT Acinetobacter baumannii strain A118

72.644

95.087

0.691
  pilT Acinetobacter baumannii D1279779

72.644

95.087

0.691
  pilT Pseudomonas stutzeri DSM 10701

70

98.266

0.688
  pilT Pseudomonas aeruginosa PAK

69.733

97.399

0.679
  pilT Legionella pneumophila strain Lp02

69.605

95.087

0.662
  pilT Legionella pneumophila strain ERS1305867

69.605

95.087

0.662
  pilT Neisseria meningitidis 8013

62.609

99.711

0.624
  pilT Neisseria gonorrhoeae MS11

62.319

99.711

0.621
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.096

96.532

0.503
  pilU Vibrio cholerae strain A1552

43.574

92.197

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.419

96.532

0.39
  pilU Acinetobacter baylyi ADP1

40.312

92.486

0.373