Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JKJ11_RS14995 Genome accession   NZ_CP068024
Coordinates   3208692..3209801 (+) Length   369 a.a.
NCBI ID   WP_252038403.1    Uniprot ID   -
Organism   Vibrio sp. SCSIO 43133     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3203692..3214801
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JKJ11_RS14960 (JKJ11_14950) - 3204058..3204660 (-) 603 WP_252038398.1 XTP/dITP diphosphatase -
  JKJ11_RS14965 (JKJ11_14955) - 3204709..3205140 (-) 432 WP_062459978.1 DUF4426 domain-containing protein -
  JKJ11_RS14970 (JKJ11_14960) yggU 3205165..3205452 (-) 288 WP_006075534.1 DUF167 family protein YggU -
  JKJ11_RS14975 (JKJ11_14965) - 3205452..3206009 (-) 558 WP_006075533.1 YggT family protein -
  JKJ11_RS14980 (JKJ11_14970) proC 3206041..3206859 (-) 819 WP_006075532.1 pyrroline-5-carboxylate reductase -
  JKJ11_RS14985 (JKJ11_14975) - 3206906..3207616 (-) 711 WP_252038400.1 YggS family pyridoxal phosphate-dependent enzyme -
  JKJ11_RS14990 (JKJ11_14980) pilT 3207642..3208682 (+) 1041 WP_252038401.1 type IV pilus twitching motility protein PilT Machinery gene
  JKJ11_RS14995 (JKJ11_14985) pilU 3208692..3209801 (+) 1110 WP_252038403.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JKJ11_RS15025 (JKJ11_15015) - 3210679..3210819 (-) 141 WP_252038404.1 hypothetical protein -
  JKJ11_RS26105 (JKJ11_15020) - 3210940..3211119 (-) 180 WP_309245327.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41337.01 Da        Isoelectric Point: 6.0940

>NTDB_id=526345 JKJ11_RS14995 WP_252038403.1 3208692..3209801(+) (pilU) [Vibrio sp. SCSIO 43133]
MHNIDTILNHMLDEKASDLYLTVDAPLLYRVDGELQAKDDKLTTEAIQALLSSVMERSKYSEYQTTKEANFAIVKPYGRF
RVSAFYQRELPGAVIRRIETQIPTFEQLSLPEVLQDLAIAKRGLVLIVGATGSGKSTTMAAMTGYRNQHRSGHILTVEDP
IEFVHEHAKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMSFAETGHLCIATLHANNASQALERIL
HLTPEDRREQFLFDLSSNLKGIVGQQLIRDKNGQGRHGVFEVLLNSPRISELIRKSDLHEIKAAIAKGKEVGMQTFDQAL
FDLVSAGKINQEDALHSADSRNDLRLMLKLQSNDNNSSSNLSDIKIDMS

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=526345 JKJ11_RS14995 WP_252038403.1 3208692..3209801(+) (pilU) [Vibrio sp. SCSIO 43133]
ATGCACAACATTGATACCATTCTAAATCACATGCTTGACGAAAAAGCCTCTGATCTTTACTTAACAGTCGATGCGCCACT
GTTATATCGCGTAGATGGCGAACTGCAAGCAAAAGATGACAAGCTCACTACAGAGGCGATTCAAGCGCTTTTAAGCAGCG
TTATGGAGCGTTCTAAGTACTCGGAGTATCAGACAACAAAAGAAGCTAATTTTGCGATAGTGAAGCCATACGGCCGTTTT
AGAGTAAGTGCATTCTACCAGCGAGAATTGCCTGGTGCAGTCATCCGAAGAATTGAAACTCAGATACCGACCTTCGAGCA
GCTTTCGTTGCCAGAAGTATTGCAAGATCTAGCGATTGCCAAACGTGGCTTAGTCTTAATAGTAGGTGCTACTGGCTCTG
GTAAATCTACCACTATGGCAGCGATGACAGGTTATCGAAATCAGCACCGCAGTGGTCATATTCTTACCGTCGAAGATCCC
ATCGAATTCGTTCATGAGCACGCAAAATGTATCGTGACACAGCGTGAGGTTGGGCTGGATACTGAAAGCTATGAAGTTGC
TCTTAAAAATTCACTTCGACAAGCTCCTGATATGATTTTAATTGGTGAGATACGTTCTAGGGAAACGATGGAATACGCCA
TGTCGTTTGCTGAAACTGGGCACCTTTGTATCGCAACATTGCACGCGAATAATGCTAGCCAAGCTTTAGAGCGAATACTA
CACCTTACACCGGAAGATAGACGGGAACAGTTCCTCTTTGACTTATCTTCGAATTTAAAAGGCATCGTAGGACAACAGCT
CATTCGAGATAAGAATGGACAGGGCCGTCACGGTGTTTTCGAGGTTCTCCTAAATAGTCCTCGTATTTCCGAGCTCATCC
GTAAAAGTGATTTACATGAAATCAAAGCTGCAATTGCGAAAGGTAAAGAAGTCGGAATGCAAACCTTTGATCAGGCCTTA
TTTGATTTGGTTTCAGCTGGCAAAATAAATCAAGAGGACGCTTTGCACAGTGCTGATTCGCGAAATGATCTCAGATTAAT
GCTAAAGCTGCAAAGTAACGACAATAACAGCTCATCAAACCTTAGCGATATTAAGATTGATATGAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

74.247

98.916

0.734
  pilU Pseudomonas stutzeri DSM 10701

56.41

95.122

0.537
  pilU Acinetobacter baylyi ADP1

52.909

97.832

0.518
  pilT Acinetobacter baylyi ADP1

40

92.141

0.369
  pilT Legionella pneumophila strain ERS1305867

39.941

91.599

0.366
  pilT Acinetobacter nosocomialis M2

39.941

91.599

0.366
  pilT Legionella pneumophila strain Lp02

39.941

91.599

0.366
  pilT Pseudomonas aeruginosa PAK

39.941

91.599

0.366
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.645

91.599

0.363
  pilT Acinetobacter baumannii D1279779

39.645

91.599

0.363
  pilT Acinetobacter baumannii strain A118

39.645

91.599

0.363
  pilT Vibrio cholerae strain A1552

40.24

90.244

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

90.244

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.181

92.683

0.363