Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JHW38_RS22410 Genome accession   NZ_CP067395
Coordinates   5316543..5317580 (+) Length   345 a.a.
NCBI ID   WP_207523495.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5311543..5322580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW38_RS22380 (JHW38_22380) - 5312133..5313077 (+) 945 WP_207523490.1 triacylglycerol lipase -
  JHW38_RS22385 (JHW38_22385) - 5313081..5314103 (+) 1023 WP_207523491.1 lipase secretion chaperone -
  JHW38_RS22390 (JHW38_22390) - 5314119..5314580 (-) 462 WP_207523492.1 DUF4426 domain-containing protein -
  JHW38_RS22395 (JHW38_22395) proC 5314577..5315416 (-) 840 WP_207523493.1 pyrroline-5-carboxylate reductase -
  JHW38_RS22400 (JHW38_22400) - 5315437..5315640 (-) 204 WP_207523494.1 hypothetical protein -
  JHW38_RS22405 (JHW38_22405) - 5315637..5316341 (-) 705 WP_242691048.1 YggS family pyridoxal phosphate-dependent enzyme -
  JHW38_RS22410 (JHW38_22410) pilT 5316543..5317580 (+) 1038 WP_207523495.1 type IV pilus twitching motility protein PilT Machinery gene
  JHW38_RS22415 (JHW38_22415) pilU 5317696..5318862 (+) 1167 WP_207523496.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JHW38_RS22420 (JHW38_22420) - 5318978..5319469 (+) 492 WP_207523497.1 hypothetical protein -
  JHW38_RS22425 (JHW38_22425) - 5319459..5320355 (-) 897 WP_207523498.1 hypothetical protein -
  JHW38_RS22430 (JHW38_22430) - 5320729..5321886 (+) 1158 WP_207523499.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38473.32 Da        Isoelectric Point: 6.7271

>NTDB_id=525858 JHW38_RS22410 WP_207523495.1 5316543..5317580(+) (pilT) [Lysobacter enzymogenes strain CX03]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLSLEDLGCPPIFRQLIEQPQGLILVTGPTGSGKSTTLAAMIDHINKSEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQAYGMMTLDQHL
QDLVKRGLILRPQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=525858 JHW38_RS22410 WP_207523495.1 5316543..5317580(+) (pilT) [Lysobacter enzymogenes strain CX03]
ATGGATATCGCCGAACTTCTGGCGTTTTCGGTAAAGAACAAAGCATCCGACTTGCATCTTTCGGCCGGGCTGCCGCCGAT
GATCCGGGTCGACGGCGACGTGCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTGCACGCCCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAAGAATTCCTGGAAGTCGACTTCTCGTTCGAGATCCCGGGCCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGTACCATTCCCTCCGAGGTCCTGAGCCTCGA
AGACCTGGGCTGTCCGCCGATCTTCCGCCAGCTGATCGAACAGCCGCAGGGCCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAGCGAGTACGCGCACATCCTCTCGGTCGAAGAC
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTCATCAACCAGCGCGAAGTCCACCGCGACACGCACGGGTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAAGATCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCGTGGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCGGAATCGTTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGCACCGCGGCCTGGGAGATCATGGTCGGCATCCCCGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGGCCTACGGCATGATGACCCTGGACCAGCACCTG
CAAGACCTGGTCAAGCGCGGCCTGATCCTGCGCCCGCAGGCCAAGGAATACGCCAAGGACAAGCGTCTGTTCGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.909

95.362

0.667
  pilT Vibrio cholerae strain A1552

69.909

95.362

0.667
  pilT Neisseria meningitidis 8013

66.569

98.841

0.658
  pilT Neisseria gonorrhoeae MS11

66.276

98.841

0.655
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Acinetobacter baylyi ADP1

40

98.551

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.606

95.652

0.388
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38