Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JHW38_RS22415 Genome accession   NZ_CP067395
Coordinates   5317696..5318862 (+) Length   388 a.a.
NCBI ID   WP_207523496.1    Uniprot ID   -
Organism   Lysobacter enzymogenes strain CX03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5312696..5323862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JHW38_RS22385 (JHW38_22385) - 5313081..5314103 (+) 1023 WP_207523491.1 lipase secretion chaperone -
  JHW38_RS22390 (JHW38_22390) - 5314119..5314580 (-) 462 WP_207523492.1 DUF4426 domain-containing protein -
  JHW38_RS22395 (JHW38_22395) proC 5314577..5315416 (-) 840 WP_207523493.1 pyrroline-5-carboxylate reductase -
  JHW38_RS22400 (JHW38_22400) - 5315437..5315640 (-) 204 WP_207523494.1 hypothetical protein -
  JHW38_RS22405 (JHW38_22405) - 5315637..5316341 (-) 705 WP_242691048.1 YggS family pyridoxal phosphate-dependent enzyme -
  JHW38_RS22410 (JHW38_22410) pilT 5316543..5317580 (+) 1038 WP_207523495.1 type IV pilus twitching motility protein PilT Machinery gene
  JHW38_RS22415 (JHW38_22415) pilU 5317696..5318862 (+) 1167 WP_207523496.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JHW38_RS22420 (JHW38_22420) - 5318978..5319469 (+) 492 WP_207523497.1 hypothetical protein -
  JHW38_RS22425 (JHW38_22425) - 5319459..5320355 (-) 897 WP_207523498.1 hypothetical protein -
  JHW38_RS22430 (JHW38_22430) - 5320729..5321886 (+) 1158 WP_207523499.1 ATP-grasp domain-containing protein -
  JHW38_RS22435 (JHW38_22435) - 5321883..5322995 (+) 1113 WP_207523500.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43006.38 Da        Isoelectric Point: 6.8286

>NTDB_id=525859 JHW38_RS22415 WP_207523496.1 5317696..5318862(+) (pilU) [Lysobacter enzymogenes strain CX03]
MNTPAPTNPTTASGAIDFTSFLKLMAHQRASDLFITAGMPPSMKVHGKIAPITQNPLTPQQSRDLVLNVMSPQQREEFEK
THECNFAIGVTGVGRFRVSCFYQRNQVGMVLRRIETKIPTVEELNLPPIIKTLAMTKRGIIIFVGATGTGKSTSLAAMIG
YRNQNSTGHIITIEDPIEFVHRHEGCIITQREVGIDTDSWDAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVL
CTLHANNANQAMDRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEILLGTPLAQDYIRDGEIHKLKEL
MKESTQLGMKTFDQALFELYQGGEISYEDALRYADSQNEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=525859 JHW38_RS22415 WP_207523496.1 5317696..5318862(+) (pilU) [Lysobacter enzymogenes strain CX03]
ATGAACACTCCCGCCCCGACCAATCCGACCACCGCCTCCGGTGCGATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCA
CCAGCGCGCCTCCGACTTGTTCATCACCGCCGGCATGCCGCCGTCGATGAAGGTGCACGGCAAGATCGCGCCGATCACCC
AGAACCCGCTGACGCCGCAGCAGAGCCGCGACCTGGTGCTCAACGTGATGAGCCCGCAGCAGCGCGAAGAATTCGAAAAG
ACCCACGAGTGCAACTTCGCCATCGGCGTCACCGGCGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGT
CGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCCGACCGTCGAGGAGCTGAACCTGCCGCCGATCATCAAGACGCTGG
CGATGACCAAGCGCGGCATCATCATCTTCGTCGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGC
TACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAATTCGTCCACCGCCACGAGGGCTGCAT
CATCACCCAGCGCGAGGTCGGCATCGACACCGACAGCTGGGACGCCGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACG
TGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTG
TGCACCCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCGGGCAGCT
GCTGATGGACTTGTCGCTGAACCTCAAGGGCGTGGTCGCCCAGCAGCTGATCCCGACCCCCGACGGCAAGGGCCGCCGCG
TGGCGATGGAGATCCTGCTCGGCACCCCGCTGGCGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGCTG
ATGAAGGAATCGACCCAGCTCGGCATGAAGACCTTCGACCAGGCCCTGTTCGAGCTGTACCAGGGCGGCGAGATCAGCTA
CGAAGACGCGCTGCGCTATGCGGACTCGCAGAACGAGGTGCGCCTGCGGATCAAGCTGGCGCAGGGCGGCGATGCGCGGA
CCTTGTCGCAGGGGTTGGATGGGGTCGAGGTGGCTGAGGTGCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-284)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.21

94.588

0.655
  pilU Acinetobacter baylyi ADP1

64.426

92.01

0.593
  pilU Vibrio cholerae strain A1552

53.371

91.753

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.165

90.464

0.381