Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JGR64_RS02955 Genome accession   NZ_CP067112
Coordinates   649613..650650 (+) Length   345 a.a.
NCBI ID   WP_199375037.1    Uniprot ID   -
Organism   Luteimonas sp. MC1572     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 644613..655650
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR64_RS02920 (JGR64_02920) - 644613..645461 (-) 849 WP_199375030.1 kinase -
  JGR64_RS02925 (JGR64_02925) pdxH 645528..646124 (+) 597 WP_199375031.1 pyridoxamine 5'-phosphate oxidase -
  JGR64_RS02930 (JGR64_02930) - 646121..646951 (+) 831 WP_234446988.1 cobalamin-binding protein -
  JGR64_RS02935 (JGR64_02935) - 647001..647396 (+) 396 WP_199375033.1 DoxX family protein -
  JGR64_RS02940 (JGR64_02940) - 647406..647879 (-) 474 WP_199375034.1 DUF4426 domain-containing protein -
  JGR64_RS02945 (JGR64_02945) proC 647887..648732 (-) 846 WP_199375035.1 pyrroline-5-carboxylate reductase -
  JGR64_RS02950 (JGR64_02950) - 648783..649475 (-) 693 WP_199375036.1 YggS family pyridoxal phosphate-dependent enzyme -
  JGR64_RS02955 (JGR64_02955) pilT 649613..650650 (+) 1038 WP_199375037.1 type IV pilus twitching motility protein PilT Machinery gene
  JGR64_RS02960 (JGR64_02960) pilU 650663..651793 (+) 1131 WP_182822668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JGR64_RS02965 (JGR64_02965) - 651909..652535 (+) 627 WP_199375038.1 superoxide dismutase -
  JGR64_RS02970 (JGR64_02970) - 653054..653515 (+) 462 WP_199375039.1 peptidylprolyl isomerase -
  JGR64_RS02975 (JGR64_02975) - 653555..655402 (-) 1848 WP_233348306.1 DUF4153 domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38542.30 Da        Isoelectric Point: 6.7836

>NTDB_id=525090 JGR64_RS02955 WP_199375037.1 649613..650650(+) (pilT) [Luteimonas sp. MC1572]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHSLVYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTVPSEVLTLEDLGTPRIFKELIDQPQGLILVTGPTGSGKSTTLAAMVDHINKTEYGHLLTIED
PIEFVHTAQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGIPAIRNLIREDKVAQMYSAIQTGQQHGMMTLDQHL
QDLVKRGMVTRPQAKDYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=525090 JGR64_RS02955 WP_199375037.1 649613..650650(+) (pilT) [Luteimonas sp. MC1572]
ATGGATATCGCTGAACTGCTGGCATTCTCGGTCAAGAACAAGGCGTCCGACCTGCACCTGTCCGCGGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTCCGCCGGATCAACATCCCGGCGTTGGACCACAAACAGGTCCATTCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCCGGCCTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGGCGCGGTGTTCCGCACGGTGCCCTCCGAGGTGCTGACGCTCGA
AGACCTCGGCACGCCGCGGATCTTCAAGGAGCTCATCGACCAGCCGCAGGGCCTGATCCTGGTCACCGGTCCCACCGGCT
CCGGCAAGTCCACCACGCTTGCGGCCATGGTCGACCACATCAACAAGACCGAATACGGGCACCTCCTCACCATCGAGGAC
CCGATCGAGTTCGTGCACACCGCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAGCTGCGCGACCTGGAGACCATCCGCCTGG
CGTTGACCGCCGCGGAAACCGGGCATCTCGTGTTCGGCACGCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGTTCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGGCGCACGGCGGCCTGGGAGATCATGGTCGGCATCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCAGCCATCCAGACCGGCCAGCAGCACGGCATGATGACCCTCGACCAGCATCTC
CAGGACCTGGTGAAGCGCGGCATGGTCACGCGGCCGCAGGCCAAGGATTACGCCAAGGACAAGCGGCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

77.581

98.261

0.762
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.429

95.362

0.681
  pilT Vibrio cholerae strain A1552

71.429

95.362

0.681
  pilT Neisseria meningitidis 8013

64.327

99.13

0.638
  pilT Neisseria gonorrhoeae MS11

64.035

99.13

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

39.655

100

0.4
  pilU Vibrio cholerae strain A1552

38.793

100

0.391
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388