Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JGR64_RS02960 Genome accession   NZ_CP067112
Coordinates   650663..651793 (+) Length   376 a.a.
NCBI ID   WP_182822668.1    Uniprot ID   A0A7X0QNJ0
Organism   Luteimonas sp. MC1572     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645663..656793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JGR64_RS02930 (JGR64_02930) - 646121..646951 (+) 831 WP_234446988.1 cobalamin-binding protein -
  JGR64_RS02935 (JGR64_02935) - 647001..647396 (+) 396 WP_199375033.1 DoxX family protein -
  JGR64_RS02940 (JGR64_02940) - 647406..647879 (-) 474 WP_199375034.1 DUF4426 domain-containing protein -
  JGR64_RS02945 (JGR64_02945) proC 647887..648732 (-) 846 WP_199375035.1 pyrroline-5-carboxylate reductase -
  JGR64_RS02950 (JGR64_02950) - 648783..649475 (-) 693 WP_199375036.1 YggS family pyridoxal phosphate-dependent enzyme -
  JGR64_RS02955 (JGR64_02955) pilT 649613..650650 (+) 1038 WP_199375037.1 type IV pilus twitching motility protein PilT Machinery gene
  JGR64_RS02960 (JGR64_02960) pilU 650663..651793 (+) 1131 WP_182822668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JGR64_RS02965 (JGR64_02965) - 651909..652535 (+) 627 WP_199375038.1 superoxide dismutase -
  JGR64_RS02970 (JGR64_02970) - 653054..653515 (+) 462 WP_199375039.1 peptidylprolyl isomerase -
  JGR64_RS02975 (JGR64_02975) - 653555..655402 (-) 1848 WP_233348306.1 DUF4153 domain-containing protein -
  JGR64_RS02980 (JGR64_02980) - 655488..656048 (+) 561 WP_199375040.1 YqgE/AlgH family protein -
  JGR64_RS02985 (JGR64_02985) ruvX 656048..656509 (+) 462 WP_199375041.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41886.21 Da        Isoelectric Point: 6.8287

>NTDB_id=525091 JGR64_RS02960 WP_182822668.1 650663..651793(+) (pilU) [Luteimonas sp. MC1572]
MSAIDFTSFLKLMAHQKASDLFITAGMPPSMKVHGKISPITQNPLTPQQARDLVLNVMSPPQREEFERTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRGQLLMDLSLNLKGVVAQQLIPTPDGKGRRVAMEIMLGTPLVQDYIRDGEIHKLKEVMKESTNLGMKTF
DQALFELYQAGEISYEDALRYADSANEVRLRIKLAQGGDARTLSQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=525091 JGR64_RS02960 WP_182822668.1 650663..651793(+) (pilU) [Luteimonas sp. MC1572]
ATGAGCGCCATCGATTTCACCTCGTTCCTCAAACTGATGGCCCACCAGAAGGCCTCGGACCTGTTCATCACCGCCGGCAT
GCCGCCGTCGATGAAGGTCCACGGCAAGATCTCGCCCATCACCCAGAACCCGCTCACGCCGCAGCAGGCGCGCGACCTGG
TGCTCAACGTGATGTCGCCGCCGCAGCGCGAGGAGTTCGAGCGCACGCACGAATGCAACTTCGCCATCGGCGTCTCGGGC
GTGGGCCGTTTCCGTGTGTCGTGCTTCTACCAGCGCAACCAGGTGGGCATGGTCTTGCGCCGCATCGAAACCAGGATCCC
GACCGTCGAGGAGCTCAACCTCCCGCCGATCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTTGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAACTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGTGAAGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTCAAGAACACCCTGCGCCAGGCGCCCGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCCATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGTCGCGGCCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCCACGCCGGACGGCAAGGGGCGGCGCGTGGCGATGGAAATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAGTCGACCAACCTCGGCATGAAGACCTTC
GACCAGGCGCTGTTCGAGCTTTACCAGGCCGGCGAGATCTCCTACGAGGATGCGCTGCGCTACGCCGACTCCGCCAACGA
GGTGCGCCTGCGCATCAAGCTGGCGCAGGGTGGCGACGCGCGCACGCTGTCGCAGGGGCTGGACGGGGTCGAGGTCGCCG
AGGTGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X0QNJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.392

97.606

0.668
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

54.494

94.681

0.516
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.109

90.691

0.391
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Legionella pneumophila strain Lp02

40.597

89.096

0.362
  pilT Legionella pneumophila strain ERS1305867

40.597

89.096

0.362
  pilT Acinetobacter nosocomialis M2

40.597

89.096

0.362
  pilT Acinetobacter baumannii D1279779

40.597

89.096

0.362
  pilT Acinetobacter baumannii strain A118

40.597

89.096

0.362