Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JFY49_RS03695 Genome accession   NZ_CP067019
Coordinates   800166..801203 (-) Length   345 a.a.
NCBI ID   WP_086196416.1    Uniprot ID   -
Organism   Acinetobacter sp. CS-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795166..806203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFY49_RS03665 (JFY49_03665) - 795948..796403 (+) 456 WP_086196421.1 bacteriohemerythrin -
  JFY49_RS03670 (JFY49_03670) - 796546..797628 (+) 1083 WP_200223828.1 hypothetical protein -
  JFY49_RS03675 (JFY49_03675) - 797628..797951 (+) 324 WP_180043000.1 RnfH family protein -
  JFY49_RS03680 (JFY49_03680) - 797997..798395 (-) 399 WP_200223829.1 outer membrane protein assembly factor BamE -
  JFY49_RS03685 (JFY49_03685) fur 798508..798945 (+) 438 WP_086175193.1 ferric iron uptake transcriptional regulator -
  JFY49_RS03690 (JFY49_03690) pilU 799018..800139 (-) 1122 WP_200223830.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JFY49_RS03695 (JFY49_03695) pilT 800166..801203 (-) 1038 WP_086196416.1 type IV pilus twitching motility protein PilT Machinery gene
  JFY49_RS03700 (JFY49_03700) - 801327..802013 (+) 687 WP_200223832.1 YggS family pyridoxal phosphate-dependent enzyme -
  JFY49_RS03705 (JFY49_03705) - 802175..802972 (+) 798 WP_200223834.1 thioesterase family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38241.92 Da        Isoelectric Point: 6.6655

>NTDB_id=524468 JFY49_RS03695 WP_086196416.1 800166..801203(-) (pilT) [Acinetobacter sp. CS-2]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICNYPRGIVLVTGPTGSGKSTTLAAMIDYINDQRYDHILTVED
PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVAKGVISAQTARTMAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=524468 JFY49_RS03695 WP_086196416.1 800166..801203(-) (pilT) [Acinetobacter sp. CS-2]
ATGGATATCACTGAACTTTTAGCGTTTTCAGCTAAAAACGGGGCATCAGATTTACACTTGTCTGCGGGGATGCCGCCACT
GATCCGTGTCGATGGTGAAGTTCGCCGTATTAATCTGCCTGCTTTAGAACATAAAGAAGTACACAAACTTGTGTATGACA
TTATGAATGATAAACAGCGTCGTGATTTTGAAGAAAAGCTAGAAACAGACTTTTCCTTTGAAGTTCCAGGTGTAGCCCGT
TTCCGTGTGAATGCATTTAACCAGAACCGTGGTGCAGGTGCTGTGTTCCGTACCATTCCTTCAAAAGTCCTCACCATTGA
AGATCTGGGCATGGGTCAGATCTTTAAAGATATCTGTAATTACCCGCGCGGTATCGTACTGGTGACCGGACCTACAGGTT
CAGGTAAATCTACTACGCTGGCGGCCATGATCGACTATATTAACGATCAGCGTTATGACCATATCCTGACGGTAGAAGAC
CCGATCGAATTTGTACACGAATCTAAAAAATGCCTGGTCAACCAGCGTGAAGTGCATCGCGATACCCATGGCTTTAATGA
AGCTCTTCGTTCAGCACTACGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATTCGTTTGG
CCTTGACTGCTGCTGAAACAGGTCACCTGGTGTTCGGTACACTACATACCACCTCTGCGGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCTGAAGAAAAAGACATGGTACGTGCCATGTTGTCTGAATCCTTGCAAGCTGTTATTTCACAAAC
CCTGCTCAAGAAAAACGGTGGCGGCCGTGTCGCTGCACACGAAATCATGATTGGTATTCCTGCCATTCGTAACCTGATCC
GTGAAAACAAAGTGGCGCAAATGTATTCTGCCATTCAAACCGGTGCCAACTACGGTATGACCACGCTTGATCAAAGTTTA
AAAACCTTGGTTGCAAAAGGTGTGATCAGTGCGCAAACTGCCCGCACAATGGCAAAACAGCCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

84.884

99.71

0.846
  pilT Pseudomonas stutzeri DSM 10701

84.593

99.71

0.843
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.643

97.391

0.678
  pilT Vibrio cholerae strain A1552

69.643

97.391

0.678
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.388

97.101

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394