Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JFY49_RS03690 Genome accession   NZ_CP067019
Coordinates   799018..800139 (-) Length   373 a.a.
NCBI ID   WP_200223830.1    Uniprot ID   A0A7T7R856
Organism   Acinetobacter sp. CS-2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 794018..805139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFY49_RS03660 (JFY49_03660) - 794682..795530 (+) 849 WP_086196422.1 class II glutamine amidotransferase -
  JFY49_RS03665 (JFY49_03665) - 795948..796403 (+) 456 WP_086196421.1 bacteriohemerythrin -
  JFY49_RS03670 (JFY49_03670) - 796546..797628 (+) 1083 WP_200223828.1 hypothetical protein -
  JFY49_RS03675 (JFY49_03675) - 797628..797951 (+) 324 WP_180043000.1 RnfH family protein -
  JFY49_RS03680 (JFY49_03680) - 797997..798395 (-) 399 WP_200223829.1 outer membrane protein assembly factor BamE -
  JFY49_RS03685 (JFY49_03685) fur 798508..798945 (+) 438 WP_086175193.1 ferric iron uptake transcriptional regulator -
  JFY49_RS03690 (JFY49_03690) pilU 799018..800139 (-) 1122 WP_200223830.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JFY49_RS03695 (JFY49_03695) pilT 800166..801203 (-) 1038 WP_086196416.1 type IV pilus twitching motility protein PilT Machinery gene
  JFY49_RS03700 (JFY49_03700) - 801327..802013 (+) 687 WP_200223832.1 YggS family pyridoxal phosphate-dependent enzyme -
  JFY49_RS03705 (JFY49_03705) - 802175..802972 (+) 798 WP_200223834.1 thioesterase family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41923.15 Da        Isoelectric Point: 6.7757

>NTDB_id=524467 JFY49_RS03690 WP_200223830.1 799018..800139(-) (pilU) [Acinetobacter sp. CS-2]
MDFNDLLNLMIQQKASDLFITADVEPSMKINGQIVPIAKTKLTGEIVGQLLNSIMSDKQRKEFAETRECNFAINNRDKTA
RFRVSAFQQRDQPGMVLRRIETVIPTIDDLKLPPILKELAMNKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHNQLFMDLSLNLRAIVAQQLIPTIDGNSRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGDRLMRASSNITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=524467 JFY49_RS03690 WP_200223830.1 799018..800139(-) (pilU) [Acinetobacter sp. CS-2]
ATGGATTTTAATGACTTGCTCAATTTGATGATTCAACAGAAGGCATCGGACTTATTTATTACGGCCGATGTTGAACCATC
CATGAAAATTAATGGTCAGATTGTGCCCATCGCCAAAACCAAGCTGACTGGTGAAATTGTGGGACAGCTTTTAAACTCGA
TTATGAGTGATAAGCAACGTAAAGAATTTGCCGAAACACGCGAATGTAACTTCGCTATCAACAACCGCGACAAAACTGCC
CGTTTCCGTGTCAGCGCATTTCAACAGCGCGATCAACCGGGTATGGTTTTACGTCGTATTGAAACTGTAATTCCAACCAT
AGATGACCTCAAGTTACCGCCCATTTTAAAAGAACTGGCGATGAACAAACGTGGTATCATCATTTTTGTCGGTGCAACTG
GTACCGGTAAATCCACCTCTTTAGCCTCGATTATTGGTTACCGTAACCAAAATTCTAAAGGTCATATTATTACCATTGAA
GACCCGATTGAATTTATCCATGAACATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATCGATACTGACTCTTTTGA
AGTGGCCTTGAAAAACACCCTGCGTCAGGCACCAGATGTAATTCTGATTGGTGAGATTCGTTCCCGCGAAACCATGGATT
ATGCCATTGCCTTTGCCGAAACCGGCCACCTGGTTTTTGCCACCCTGCACGCCAACAACGCCAACCAGGCGATTGACCGT
ATTATTCACTTCTTTGAGGCAGACCGTCATAACCAGCTGTTTATGGATTTATCGCTCAATTTAAGAGCTATTGTAGCGCA
GCAATTGATTCCAACCATAGATGGCAACTCACGTCGTGCAGCGATTGAAATCCTGATCAATTCACCTTTAATTTCAGACC
TGATCCGTAAAGGTGATGTGCATGAAATTAAAGACCTGATGAAACGTTCACGTGAACTGGGCATGCAAACCTTTGACCAG
GCCCTGTTTGACTTGTACAAAGCCAAGCAAATTAGCTATAAAGATGCATTGAAACATGCCGACTCACCAAACGATCTGCG
TTTGCAAATCAAGTTGTCGGAAGAAGGCGGAGATCGCTTAATGCGCGCCAGTTCGAATATTACTTTCGATGGTCAGGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7T7R856

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.775
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.464

0.684
  pilU Vibrio cholerae strain A1552

54.324

99.196

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

92.761

0.394
  pilT Legionella pneumophila strain ERS1305867

41.964

90.08

0.378
  pilT Legionella pneumophila strain Lp02

41.964

90.08

0.378
  pilT Acinetobacter baumannii strain A118

40.525

91.957

0.373
  pilT Acinetobacter nosocomialis M2

40.525

91.957

0.373
  pilT Acinetobacter baumannii D1279779

40.525

91.957

0.373
  pilT Pseudomonas aeruginosa PAK

40.173

92.761

0.373
  pilT Acinetobacter baylyi ADP1

39.942

91.957

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.306

92.761

0.365
  pilT Neisseria gonorrhoeae MS11

39.706

91.153

0.362
  pilT Neisseria meningitidis 8013

39.017

92.761

0.362