NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	JH311_RS05230	Genome accession	NZ_CP066987
Coordinates	1182118..1182528 (+)	Length	136 a.a.
NCBI ID	WP_228424134.1	Uniprot ID	-
Organism	Xanthomonas campestris pv. campestris strain 42-4
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1177118..1187528

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH311_RS05200 (JH311_05205)	-	1177214..1177705 (-)	492	WP_011038220.1	GNAT family N-acetyltransferase	-
JH311_RS05205 (JH311_05210)	-	1177702..1177992 (-)	291	WP_011038219.1	DUF1778 domain-containing protein	-
JH311_RS05210 (JH311_05215)	-	1178067..1178468 (-)	402	WP_040940795.1	SymE family type I addiction module toxin	-
JH311_RS05215 (JH311_05220)	coaE	1179000..1179623 (-)	624	WP_274398949.1	dephospho-CoA kinase	-
JH311_RS05220 (JH311_05225)	-	1179637..1180500 (-)	864	WP_228876553.1	A24 family peptidase	-
JH311_RS05225 (JH311_05230)	pilC	1180507..1181766 (-)	1260	WP_228424132.1	type II secretion system F family protein	Machinery gene
JH311_RS05230 (JH311_05235)	pilA2	1182118..1182528 (+)	411	WP_228424134.1	pilin	Machinery gene
JH311_RS05235 (JH311_05240)	pilB	1182803..1184509 (+)	1707	WP_274400977.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH311_RS05240 (JH311_05245)	pilR	1184927..1186321 (-)	1395	WP_042594919.1	sigma-54 dependent transcriptional regulator	Regulator

Sequence

Protein

Download Length: 136 a.a. Molecular weight: 14119.35 Da Isoelectric Point: 9.0009

                                >NTDB_id=523925 JH311_RS05230 WP_228424134.1 1182118..1182528(+) (pilA2) [Xanthomonas campestris pv. campestris strain 42-4]

MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLVKSRVSEVMLAASSARTTVSEAAATDTATAMPATVAIETQASKYVS

SIAYKDGVITATAKDLGGSTPTGTITLTGAKQTNGQVLWTCAGTIDKKYRPATCQG

Nucleotide

Download Length: 411 bp

                                >NTDB_id=523925 JH311_RS05230 WP_228424134.1 1182118..1182528(+) (pilA2) [Xanthomonas campestris pv. campestris strain 42-4]

ATGAAGAAGCAACAAGGTTTTACCCTGATCGAATTGATGATTGTGGTTGCGATCATCGCCATCCTGGCTGCTATCGCATT

GCCGGCTTATCAGGATTACCTGGTCAAGTCGCGCGTGTCGGAGGTGATGTTGGCCGCCTCGTCCGCACGTACAACCGTCT

CAGAAGCCGCCGCTACTGACACTGCCACAGCCATGCCTGCAACTGTTGCCATTGAGACACAGGCTTCGAAGTATGTTTCC

TCGATTGCTTACAAAGATGGCGTCATCACTGCAACTGCTAAGGACCTTGGTGGAAGCACGCCGACCGGCACCATTACGCT

TACTGGTGCTAAGCAGACCAACGGTCAAGTTCTTTGGACATGCGCAGGCACTATCGATAAGAAGTATCGTCCTGCGACCT

GCCAGGGGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-135)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila strain ERS1305867	52.518	100	0.537
pilA2	Legionella pneumophila str. Paris	52.518	100	0.537
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	38.378	100	0.522
comP	Acinetobacter baylyi ADP1	44.371	100	0.493
pilA	Ralstonia pseudosolanacearum GMI1000	38.365	100	0.449
pilE	Neisseria gonorrhoeae MS11	36.25	100	0.426
pilA/pilA1	Eikenella corrodens VA1	37.662	100	0.426
pilA	Pseudomonas aeruginosa PAK	37.5	100	0.419
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.304	100	0.419
pilE	Neisseria gonorrhoeae strain FA1090	36.538	100	0.419
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.706	100	0.397
pilA	Vibrio cholerae strain A1552	36.806	100	0.39
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.39
pilA	Vibrio cholerae C6706	36.806	100	0.39
pilA	Acinetobacter baumannii strain A118	37.589	100	0.39
pilA	Vibrio parahaemolyticus RIMD 2210633	39.535	94.853	0.375

Detailed information NTG0523925