Detailed information NTG0523636

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: JH262_RS16775
• Genome accession: NZ_CP066961
• Coordinates: 3783020..3783430 (-)
• Length: 136 a.a.
• NCBI ID: WP_228424134.1
• Uniprot ID: -
• Organism: Xanthomonas campestris pv. incanae strain 5057
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3774949..3786548	3783020..3783430	within	0

Gene organization within MGE regions

Location: 3774949..3786548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH262_RS16745 (JH262_16680)	sucC	3774949..3776118 (-)	1170	WP_011038209.1	ADP-forming succinate--CoA ligase subunit beta	-
JH262_RS16750 (JH262_16685)	-	3776352..3777962 (+)	1611	WP_223646353.1	HAMP domain-containing sensor histidine kinase	-
JH262_RS16755 (JH262_16690)	pilR	3778171..3779565 (+)	1395	WP_043921909.1	sigma-54 dependent transcriptional regulator	Regulator
JH262_RS16760 (JH262_16695)	-	3779763..3780119 (+)	357	WP_139328384.1	hypothetical protein	-
JH262_RS16765 (JH262_16700)	-	3780119..3780856 (+)	738	WP_070690009.1	zeta toxin family protein	-
JH262_RS16770 (JH262_16705)	pilB	3781039..3782745 (-)	1707	WP_274393759.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH262_RS16775 (JH262_16710)	pilA2	3783020..3783430 (-)	411	WP_228424134.1	pilin	Machinery gene
JH262_RS16780 (JH262_16715)	pilC	3783782..3785041 (+)	1260	WP_228424132.1	type II secretion system F family protein	Machinery gene
JH262_RS16785 (JH262_16720)	-	3785048..3785911 (+)	864	WP_223646361.1	A24 family peptidase	-
JH262_RS16790 (JH262_16725)	coaE	3785925..3786548 (+)	624	WP_274390763.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 136 a.a.
• Molecular weight: 14119.35 Da
• Isoelectric Point: 9.0009

>NTDB_id=523636 JH262_RS16775 WP_228424134.1 3783020..3783430(-) (pilA2) [Xanthomonas campestris pv. incanae strain 5057]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLVKSRVSEVMLAASSARTTVSEAAATDTATAMPATVAIETQASKYVS
SIAYKDGVITATAKDLGGSTPTGTITLTGAKQTNGQVLWTCAGTIDKKYRPATCQG

Nucleotide

• Length: 411 bp

>NTDB_id=523636 JH262_RS16775 WP_228424134.1 3783020..3783430(-) (pilA2) [Xanthomonas campestris pv. incanae strain 5057]
ATGAAGAAGCAACAAGGTTTTACCCTGATCGAATTGATGATTGTGGTTGCGATCATCGCCATCCTGGCTGCTATCGCATT
GCCGGCTTATCAGGATTACCTGGTCAAGTCGCGCGTGTCGGAAGTGATGTTGGCCGCCTCGTCCGCACGTACAACCGTCT
CAGAAGCCGCCGCTACTGACACTGCCACAGCCATGCCTGCAACTGTTGCCATTGAGACACAGGCTTCGAAGTATGTTTCC
TCGATTGCTTACAAAGATGGCGTCATCACTGCAACTGCTAAGGACCTTGGTGGAAGCACGCCGACCGGCACCATTACGCT
TACTGGTGCTAAGCAGACCAACGGTCAAGTTCTTTGGACATGCGCAGGCACTATCGATAAGAAGTATCGTCCTGCGACCT
GCCAGGGGTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

• Pilin (bacterial filament) (Pilin) (35-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila strain ERS1305867	52.518	100	0.537
pilA2	Legionella pneumophila str. Paris	52.518	100	0.537
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	38.378	100	0.522
comP	Acinetobacter baylyi ADP1	44.371	100	0.493
pilA	Ralstonia pseudosolanacearum GMI1000	38.365	100	0.449
pilE	Neisseria gonorrhoeae MS11	36.25	100	0.426
pilA/pilA1	Eikenella corrodens VA1	37.662	100	0.426
pilA	Pseudomonas aeruginosa PAK	37.5	100	0.419
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.304	100	0.419
pilE	Neisseria gonorrhoeae strain FA1090	36.538	100	0.419
pilA/pilAII	Pseudomonas stutzeri DSM 10701	39.706	100	0.397
pilA	Vibrio cholerae strain A1552	36.806	100	0.39
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	36.806	100	0.39
pilA	Vibrio cholerae C6706	36.806	100	0.39
pilA	Acinetobacter baumannii strain A118	37.589	100	0.39
pilA	Vibrio parahaemolyticus RIMD 2210633	39.535	94.853	0.375