NTDB

Overview

Name	pilA2	Type	Machinery gene
Locus tag	JH270_RS05530	Genome accession	NZ_CP066931
Coordinates	1321592..1322023 (+)	Length	143 a.a.
NCBI ID	WP_043922199.1	Uniprot ID	-
Organism	Xanthomonas campestris pv. raphani strain 609
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1316592..1327023

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JH270_RS05500 (JH270_05500)	-	1316989..1317480 (-)	492	WP_011038220.1	GNAT family N-acetyltransferase	-
JH270_RS05505 (JH270_05505)	-	1317477..1317767 (-)	291	WP_014508712.1	DUF1778 domain-containing protein	-
JH270_RS05510 (JH270_05510)	-	1317843..1318244 (-)	402	WP_274341704.1	SymE family type I addiction module toxin	-
JH270_RS05515 (JH270_05515)	coaE	1318480..1319103 (-)	624	WP_076038488.1	dephospho-CoA kinase	-
JH270_RS05520 (JH270_05520)	-	1319117..1319980 (-)	864	WP_011038216.1	A24 family peptidase	-
JH270_RS05525 (JH270_05525)	pilC	1319987..1321243 (-)	1257	WP_274342103.1	type II secretion system F family protein	Machinery gene
JH270_RS05530 (JH270_05530)	pilA2	1321592..1322023 (+)	432	WP_043922199.1	pilin	Machinery gene
JH270_RS05535 (JH270_05535)	pilB	1322161..1323897 (+)	1737	WP_043921911.1	type IV-A pilus assembly ATPase PilB	Machinery gene
JH270_RS05540 (JH270_05540)	-	1324080..1324871 (-)	792	WP_202796265.1	toxin	-
JH270_RS05545 (JH270_05545)	-	1324816..1325178 (-)	363	WP_148263072.1	hypothetical protein	-
JH270_RS05550 (JH270_05550)	pilR	1325370..1326764 (-)	1395	WP_274341705.1	sigma-54 dependent transcriptional regulator	Regulator

Sequence

Protein

Download Length: 143 a.a. Molecular weight: 14758.07 Da Isoelectric Point: 9.0331

                                >NTDB_id=523318 JH270_RS05530 WP_043922199.1 1321592..1322023(+) (pilA2) [Xanthomonas campestris pv. raphani strain 609]

MKKQQGFTLIELMIVIAIIAILAAIALPAYQDYTIRSKVSETMIAMSAAKLAVAETAQSQGILATAVTNNTAAGYSGQTT

DKVASVVIANGVVTGTSKNTGAKVEPILKLTPTQADKDSPITWACSYTAGEAKHVPASCRTAG

Nucleotide

Download Length: 432 bp

                                >NTDB_id=523318 JH270_RS05530 WP_043922199.1 1321592..1322023(+) (pilA2) [Xanthomonas campestris pv. raphani strain 609]

ATGAAAAAGCAGCAAGGTTTTACTCTTATTGAATTGATGATTGTTATTGCCATCATCGCGATCCTAGCCGCCATTGCTCT

TCCGGCATATCAGGATTACACCATTCGAAGCAAAGTCAGCGAAACCATGATTGCTATGTCTGCAGCTAAGCTGGCGGTAG

CTGAGACTGCCCAGTCTCAAGGTATTCTGGCAACTGCCGTTACTAACAACACCGCCGCTGGATACTCCGGGCAAACTACC

GATAAGGTAGCGAGCGTGGTCATTGCCAATGGTGTGGTCACCGGTACTTCCAAGAACACTGGCGCAAAAGTTGAGCCGAT

CTTGAAGCTTACCCCCACGCAGGCTGATAAAGACTCGCCGATTACATGGGCTTGCAGCTACACCGCTGGTGAAGCTAAGC

ATGTCCCGGCAAGCTGCCGTACCGCTGGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-140)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA2	Legionella pneumophila str. Paris	51.02	100	0.524
pilA2	Legionella pneumophila strain ERS1305867	50.34	100	0.517
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	38.378	100	0.496
comP	Acinetobacter baylyi ADP1	42.767	100	0.476
pilE	Neisseria gonorrhoeae strain FA1090	40.764	100	0.448
pilA	Ralstonia pseudosolanacearum GMI1000	37.952	100	0.441
pilE	Neisseria gonorrhoeae MS11	39.873	100	0.441
pilA/pilAI	Pseudomonas stutzeri DSM 10701	40.559	100	0.406
pilA	Haemophilus influenzae 86-028NP	39.716	98.601	0.392
pilA	Haemophilus influenzae Rd KW20	38.462	100	0.385
pilA/pilA1	Eikenella corrodens VA1	36	100	0.378
pilA	Vibrio cholerae C6706	35.811	100	0.371
pilA	Vibrio cholerae strain A1552	35.811	100	0.371
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	35.811	100	0.371

Detailed information NTG0523318