Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J6O21_RS19810 Genome accession   NZ_CP081706
Coordinates   3970163..3970603 (+) Length   146 a.a.
NCBI ID   WP_000360901.1    Uniprot ID   -
Organism   Escherichia coli strain AH04     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3965163..3975603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6O21_RS19790 (J6O21_19690) aroP 3966161..3967534 (+) 1374 WP_077784498.1 aromatic amino acid transporter AroP -
  J6O21_RS19795 (J6O21_19695) ampE 3967577..3968431 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  J6O21_RS19800 (J6O21_19700) ampD 3968428..3968979 (-) 552 WP_000923727.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J6O21_RS19805 (J6O21_19705) nadC 3969067..3969960 (+) 894 WP_001135176.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J6O21_RS19810 (J6O21_19710) pilA 3970163..3970603 (+) 441 WP_000360901.1 prepilin peptidase-dependent pilin Machinery gene
  J6O21_RS19815 (J6O21_19715) pilB 3970613..3971998 (+) 1386 WP_057107789.1 type II secretion system protein GspE Machinery gene
  J6O21_RS19820 (J6O21_19720) hofC 3971988..3973190 (+) 1203 WP_000157259.1 protein transport protein HofC -
  J6O21_RS19825 (J6O21_19725) guaC 3973225..3974268 (-) 1044 WP_001217337.1 GMP reductase -
  J6O21_RS19830 (J6O21_19730) - 3974424..3974468 (-) 45 WP_120795372.1 protein YacM -
  J6O21_RS19835 (J6O21_19735) coaE 3974493..3975113 (+) 621 WP_001269531.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15648.78 Da        Isoelectric Point: 4.3938

>NTDB_id=523320 J6O21_RS19810 WP_000360901.1 3970163..3970603(+) (pilA) [Escherichia coli strain AH04]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQNDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=523320 J6O21_RS19810 WP_000360901.1 3970163..3970603(+) (pilA) [Escherichia coli strain AH04]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCTGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAATGACAGCGCATTGCAGC
AAGCCTGTGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363