Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JH270_RS05535 Genome accession   NZ_CP066931
Coordinates   1322161..1323897 (+) Length   578 a.a.
NCBI ID   WP_043921911.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 609     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1317161..1328897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH270_RS05505 (JH270_05505) - 1317477..1317767 (-) 291 WP_014508712.1 DUF1778 domain-containing protein -
  JH270_RS05510 (JH270_05510) - 1317843..1318244 (-) 402 WP_274341704.1 SymE family type I addiction module toxin -
  JH270_RS05515 (JH270_05515) coaE 1318480..1319103 (-) 624 WP_076038488.1 dephospho-CoA kinase -
  JH270_RS05520 (JH270_05520) - 1319117..1319980 (-) 864 WP_011038216.1 A24 family peptidase -
  JH270_RS05525 (JH270_05525) pilC 1319987..1321243 (-) 1257 WP_274342103.1 type II secretion system F family protein Machinery gene
  JH270_RS05530 (JH270_05530) pilA2 1321592..1322023 (+) 432 WP_043922199.1 pilin Machinery gene
  JH270_RS05535 (JH270_05535) pilB 1322161..1323897 (+) 1737 WP_043921911.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH270_RS05540 (JH270_05540) - 1324080..1324871 (-) 792 WP_202796265.1 toxin -
  JH270_RS05545 (JH270_05545) - 1324816..1325178 (-) 363 WP_148263072.1 hypothetical protein -
  JH270_RS05550 (JH270_05550) pilR 1325370..1326764 (-) 1395 WP_274341705.1 sigma-54 dependent transcriptional regulator Regulator
  JH270_RS05555 (JH270_05555) - 1326973..1328583 (-) 1611 WP_274318290.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62655.76 Da        Isoelectric Point: 5.6039

>NTDB_id=523319 JH270_RS05535 WP_043921911.1 1322161..1323897(+) (pilB) [Xanthomonas campestris pv. raphani strain 609]
MSAGVSVNLVGITGIARRLVQDGVLEEAAARSAMDQAGSAKVPLPQWFSEKKLVTAAQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASLGSSLG
DDDDDMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALM
KASHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=523319 JH270_RS05535 WP_043921911.1 1322161..1323897(+) (pilB) [Xanthomonas campestris pv. raphani strain 609]
ATGAGTGCTGGAGTTTCTGTCAATCTCGTTGGCATAACAGGAATCGCCAGGCGCTTGGTCCAAGACGGTGTTCTAGAGGA
AGCTGCTGCACGATCTGCAATGGATCAGGCGGGTAGTGCCAAAGTACCGTTGCCCCAATGGTTTTCTGAGAAAAAGTTGG
TGACGGCTGCTCAGCTAGCTGCAGCCAACGCTGTCGAGTTCGGTATGCCGCTACTTGATGTGTCGGCGTTCGACGCCAGC
CAGAATGCGGTCAAGCTAGTCAGTGAAGAGTTGCTCCAGAAATATCAAGTGCTGCCTTTGTTCAAACGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACTCAGACCAGGGCGCTGGACGATATCAAGTTCCATACGAACCTGGTGGTCGAGCCTA
TCCTGGTCGACGAGGACCAGATTCGTCGCACCTTGGAGCAGTGGCAGGCCAGCAATGCGTCGCTTGGCTCATCGCTCGGT
GACGACGATGACGACATGGGGGATTTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGATGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTATCGGGTGCGGTTGCGGATCGATGGGCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTCAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCTTGTTCGGCGAAA
AGGTTGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTCGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTATTCCTGGAGGCGATCCACAAACCCTACGGCATGGTGCTGGTGACCGGGCCGACTGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATTTCAACGGCCGAGGATCCGGTCGAAATCCGCTTGCCCG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCTTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCTTACAACATCA
CCTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCTGCGCAGATCGCTGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGTACCGAAGGCTACAAGGGCCGTACCGGCATCTATCAGGTGATGCCGATGACGGACGAAATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCTGCGCAGAAGATCGGTATCCGCGACCTGCGCCAGTCGGCGTTAATG
AAGGCTTCGCACGGCGTGACCAGCCTGGCCGAAATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.27

0.548
  pilB Acinetobacter baylyi ADP1

54.754

98.27

0.538
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilF Neisseria gonorrhoeae MS11

49.12

98.27

0.483
  pilB Vibrio cholerae strain A1552

48.174

99.481

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.25

96.886

0.448
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.924

91.003

0.363
  pilF Thermus thermophilus HB27

40.192

89.965

0.362