Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   JH270_RS05525 Genome accession   NZ_CP066931
Coordinates   1319987..1321243 (-) Length   418 a.a.
NCBI ID   WP_274342103.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 609     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1314987..1326243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH270_RS05495 (JH270_05495) - 1315512..1316846 (+) 1335 WP_012437598.1 HAMP domain-containing sensor histidine kinase -
  JH270_RS05500 (JH270_05500) - 1316989..1317480 (-) 492 WP_011038220.1 GNAT family N-acetyltransferase -
  JH270_RS05505 (JH270_05505) - 1317477..1317767 (-) 291 WP_014508712.1 DUF1778 domain-containing protein -
  JH270_RS05510 (JH270_05510) - 1317843..1318244 (-) 402 WP_274341704.1 SymE family type I addiction module toxin -
  JH270_RS05515 (JH270_05515) coaE 1318480..1319103 (-) 624 WP_076038488.1 dephospho-CoA kinase -
  JH270_RS05520 (JH270_05520) - 1319117..1319980 (-) 864 WP_011038216.1 A24 family peptidase -
  JH270_RS05525 (JH270_05525) pilC 1319987..1321243 (-) 1257 WP_274342103.1 type II secretion system F family protein Machinery gene
  JH270_RS05530 (JH270_05530) pilA2 1321592..1322023 (+) 432 WP_043922199.1 pilin Machinery gene
  JH270_RS05535 (JH270_05535) pilB 1322161..1323897 (+) 1737 WP_043921911.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JH270_RS05540 (JH270_05540) - 1324080..1324871 (-) 792 WP_202796265.1 toxin -
  JH270_RS05545 (JH270_05545) - 1324816..1325178 (-) 363 WP_148263072.1 hypothetical protein -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 45715.04 Da        Isoelectric Point: 10.3371

>NTDB_id=523317 JH270_RS05525 WP_274342103.1 1319987..1321243(-) (pilC) [Xanthomonas campestris pv. raphani strain 609]
MSAARHAMKKQVADRGSQMQSFLWEGTDKRGVKMKGEQSARNVNMLRAELRRQGIMPSSVKAKPKPLFGAAGKKITPKDI
AFFSRQMATMMKSGVPIVGSLEIIGGGHKNPRMKQMVGRVRTDIEGGSSLHEAISKHPVQFDELYRNLVRAGEGAGVLET
VLDTVASYKENIETLKGKIKKALFYPAMVVAVAIIVSAILLIFVVPQFEEVFKSFGAELPAFTQMIVNASRFMVSYWWIM
LIAGVGCAAGSIFAYKRSPRMQHSMDRLILKFPIIGQIMHNSSIARFARTTAVTFKAGVPLVEALGIVAGATGNKVYEEA
VLRMRDDVSVGYPVNMAMKQVNLFPHMVIQMTAIGEEAGALDTMLFKVAEYFEQEVNNAVDALSSLLEPLIMVFIGTIVG
GMVIGMYLPIFKLGAVVG

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=523317 JH270_RS05525 WP_274342103.1 1319987..1321243(-) (pilC) [Xanthomonas campestris pv. raphani strain 609]
ATGTCAGCGGCACGACACGCTATGAAAAAGCAAGTGGCAGACAGGGGCAGCCAAATGCAGTCGTTCCTCTGGGAGGGGAC
GGACAAGCGCGGTGTTAAGATGAAAGGCGAACAGAGTGCTCGCAATGTGAATATGTTGCGAGCCGAACTTCGTCGTCAAG
GGATCATGCCCTCCTCCGTGAAGGCCAAGCCCAAACCGCTCTTTGGTGCAGCCGGAAAAAAAATTACGCCAAAGGATATT
GCGTTCTTCAGCCGCCAGATGGCCACGATGATGAAGTCGGGTGTCCCAATTGTTGGCTCATTAGAGATTATTGGCGGAGG
ACATAAGAACCCGAGAATGAAACAGATGGTTGGTCGAGTCCGAACTGACATTGAGGGCGGATCCTCACTCCATGAAGCGA
TCAGCAAGCACCCCGTACAATTCGACGAACTTTATCGCAACCTTGTAAGAGCTGGCGAAGGAGCGGGCGTATTAGAAACA
GTCTTAGATACAGTTGCTAGCTATAAAGAAAATATCGAGACCCTAAAAGGGAAGATAAAAAAGGCTTTATTCTATCCTGC
CATGGTTGTCGCGGTAGCGATCATAGTCAGCGCAATTCTTCTTATTTTTGTCGTGCCTCAGTTTGAAGAAGTTTTTAAAA
GCTTTGGAGCTGAGCTGCCGGCTTTTACACAAATGATCGTAAATGCATCGCGCTTCATGGTTAGCTACTGGTGGATTATG
CTTATCGCAGGAGTAGGCTGCGCCGCGGGATCAATCTTTGCTTATAAACGCTCACCCCGTATGCAGCACAGCATGGATCG
ACTGATTTTGAAGTTTCCGATTATTGGTCAAATCATGCACAACAGTTCCATCGCGCGTTTTGCTAGAACCACTGCTGTTA
CTTTCAAAGCGGGCGTTCCACTAGTTGAAGCGCTCGGCATCGTAGCAGGCGCCACCGGCAACAAAGTGTACGAAGAAGCC
GTGCTGCGCATGCGGGACGACGTGTCGGTTGGCTATCCGGTCAACATGGCAATGAAGCAGGTCAACCTGTTCCCCCATAT
GGTGATCCAGATGACCGCAATCGGTGAAGAAGCAGGCGCACTGGATACGATGTTGTTCAAAGTTGCCGAGTACTTCGAGC
AGGAAGTCAACAATGCCGTTGATGCGCTCAGCAGTCTCCTTGAACCACTAATTATGGTGTTCATCGGTACTATCGTAGGC
GGCATGGTCATCGGCATGTATCTTCCGATCTTCAAACTCGGCGCAGTGGTTGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(287-409)

Type II secretion system (T2SS), protein F (T2SSF)

(83-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.03

94.737

0.502
  pilC Legionella pneumophila strain ERS1305867

51.605

96.89

0.5
  pilC Acinetobacter baylyi ADP1

51.232

97.129

0.498
  pilC Acinetobacter baumannii D1279779

50.99

96.651

0.493
  pilG Neisseria gonorrhoeae MS11

45.05

96.651

0.435
  pilG Neisseria meningitidis 44/76-A

44.802

96.651

0.433
  pilC Vibrio cholerae strain A1552

41

95.694

0.392
  pilC Vibrio campbellii strain DS40M4

38.903

95.933

0.373
  pilC Thermus thermophilus HB27

37.624

96.651

0.364