Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JDW18_RS20240 Genome accession   NZ_CP066783
Coordinates   4388371..4389414 (+) Length   347 a.a.
NCBI ID   WP_218241399.1    Uniprot ID   -
Organism   Comamonas fluminis strain CJ34     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4383371..4394414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JDW18_RS20220 - 4383643..4385241 (-) 1599 WP_218241395.1 AMP-binding protein -
  JDW18_RS20225 - 4385407..4386132 (+) 726 WP_218241396.1 TetR/AcrR family transcriptional regulator -
  JDW18_RS20230 - 4386204..4387454 (+) 1251 WP_218241397.1 branched-chain amino acid ABC transporter substrate-binding protein -
  JDW18_RS20235 - 4387602..4388333 (-) 732 WP_218241398.1 YggS family pyridoxal phosphate-dependent enzyme -
  JDW18_RS20240 pilT 4388371..4389414 (+) 1044 WP_218241399.1 type IV pilus twitching motility protein PilT Machinery gene
  JDW18_RS20245 pilU 4389563..4390699 (+) 1137 WP_218241400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JDW18_RS20250 - 4390852..4391757 (+) 906 WP_218241401.1 NAD(P)-dependent oxidoreductase -
  JDW18_RS20255 - 4391834..4392493 (-) 660 WP_218244004.1 BON domain-containing protein -
  JDW18_RS20260 - 4392499..4393107 (-) 609 WP_218241402.1 SIS domain-containing protein -
  JDW18_RS20265 - 4393186..4393575 (-) 390 WP_218241404.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38314.12 Da        Isoelectric Point: 6.7745

>NTDB_id=522517 JDW18_RS20240 WP_218241399.1 4388371..4389414(+) (pilT) [Comamonas fluminis strain CJ34]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKTVHSMVYDIMSDAQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNQNRGSAAVFRTIPSKILTLEQLNAPKIFGELALKPRGLVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPMTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTPAIRNLIREAKVAQMYSTIQTSQNVGMQTLDQN
LTDLVRRNVISAAEARSKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=522517 JDW18_RS20240 WP_218241399.1 4388371..4389414(+) (pilT) [Comamonas fluminis strain CJ34]
GTGGACATCACGCAATTGCTGGCATTCAGTGTCAAGAACAAGGCTTCGGACTTGCATTTGTCCGCAGGGCTGCCGCCCAT
GATCCGTGTGCACGGCGATGTGCGCCGCATCAATATCGATCCGCTGGACCACAAGACCGTGCACTCCATGGTGTACGACA
TCATGAGCGATGCCCAGCGCAAGCAGTACGAAGAATTTCTGGAAGTGGACTTTTCCTTCGAAATTGAAGGGCTGGCACGT
TTTCGTGTGAATGCGTTCAACCAGAACCGTGGCTCTGCCGCCGTCTTTCGTACCATTCCCAGCAAGATTCTGACGCTGGA
GCAGCTCAACGCGCCCAAGATTTTTGGCGAACTGGCCCTGAAGCCACGCGGCTTGGTGCTGGTGACTGGCCCCACGGGTT
CGGGTAAATCCACCACGCTGGCGGCCATGGTCAACTACCTGAATGAAACCGAATACGGCCACATTCTGACGGTGGAAGAC
CCTATCGAATTCGTGCACGAATCCAAGAAGTGCCTGATCAACCAGCGCGAAGTCGGCCCCATGACGCTGTCGTTCGCCGC
CGCGCTGAAATCTGCACTGCGTGAAGACCCAGACGCCATTCTGGTTGGTGAAATGCGTGACCTGGAAACCATTCGCCTGG
CCATGACCGCTGCCGAAACTGGCCACCTGGTGTTTGGCACGCTGCACACTTCATCAGCCGCCAAAACCATCGACCGTATC
ATCGACGTCTTCCCCGCCGAAGAAAAAGAAATGGTGCGCGCGATGTTGTCCGAATCGCTGCAGGCCGTGATTTCGCAGAC
GCTGTGCAAGACCAAGGACGGCCAGGGCCGCGTGGCCGCGCACGAAATCATGCTGGGCACGCCCGCCATTCGTAACCTGA
TTCGCGAGGCCAAGGTGGCGCAGATGTACTCCACCATCCAGACCAGCCAGAACGTGGGCATGCAGACGCTGGACCAGAAC
CTGACGGACCTGGTGCGCCGCAACGTCATCAGCGCAGCCGAGGCACGCAGCAAGGCGAAGATTCCTGAGAACTTCCCTGG
GTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

76.232

99.424

0.758
  pilT Acinetobacter baumannii D1279779

76.232

99.424

0.758
  pilT Acinetobacter baumannii strain A118

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

75.362

99.424

0.749
  pilT Acinetobacter baylyi ADP1

74.493

99.424

0.741
  pilT Legionella pneumophila strain Lp02

74.041

97.695

0.723
  pilT Legionella pneumophila strain ERS1305867

74.041

97.695

0.723
  pilT Neisseria gonorrhoeae MS11

68.406

99.424

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.035

98.271

0.669
  pilT Vibrio cholerae strain A1552

68.035

98.271

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

99.424

0.504
  pilU Vibrio cholerae strain A1552

44.478

96.542

0.429
  pilU Acinetobacter baylyi ADP1

42.197

99.712

0.421
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.542

0.406