Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JDW18_RS20245 Genome accession   NZ_CP066783
Coordinates   4389563..4390699 (+) Length   378 a.a.
NCBI ID   WP_218241400.1    Uniprot ID   -
Organism   Comamonas fluminis strain CJ34     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4384563..4395699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JDW18_RS20225 - 4385407..4386132 (+) 726 WP_218241396.1 TetR/AcrR family transcriptional regulator -
  JDW18_RS20230 - 4386204..4387454 (+) 1251 WP_218241397.1 branched-chain amino acid ABC transporter substrate-binding protein -
  JDW18_RS20235 - 4387602..4388333 (-) 732 WP_218241398.1 YggS family pyridoxal phosphate-dependent enzyme -
  JDW18_RS20240 pilT 4388371..4389414 (+) 1044 WP_218241399.1 type IV pilus twitching motility protein PilT Machinery gene
  JDW18_RS20245 pilU 4389563..4390699 (+) 1137 WP_218241400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JDW18_RS20250 - 4390852..4391757 (+) 906 WP_218241401.1 NAD(P)-dependent oxidoreductase -
  JDW18_RS20255 - 4391834..4392493 (-) 660 WP_218244004.1 BON domain-containing protein -
  JDW18_RS20260 - 4392499..4393107 (-) 609 WP_218241402.1 SIS domain-containing protein -
  JDW18_RS20265 - 4393186..4393575 (-) 390 WP_218241404.1 YraN family protein -
  JDW18_RS20270 rsmI 4393575..4394495 (+) 921 WP_218241405.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41963.39 Da        Isoelectric Point: 6.7687

>NTDB_id=522518 JDW18_RS20245 WP_218241400.1 4389563..4390699(+) (pilU) [Comamonas fluminis strain CJ34]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSQLHTLALARSIMSDKQVADFEHTKECNFAI
SPAGVGRFRVNAFVQQGKVGMVLRTIPTTLPTIDGLGVPQILKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNEQSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTESWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDISLNLRAIVSQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVAEIKEIMKKSRNVG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTQDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=522518 JDW18_RS20245 WP_218241400.1 4389563..4390699(+) (pilU) [Comamonas fluminis strain CJ34]
ATGGAACGCGATCAGGCCAGTAAATTTATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGTGACCTGTTCAT
CACGGCGGAGTTTCCGCCCGCTATCAAGATTGACGGCAAGGTGACCAAGGTGTCGCCGCAGCCGCTGTCGCAGTTGCACA
CGCTGGCACTGGCGCGCTCCATCATGAGCGACAAGCAGGTGGCCGACTTTGAACACACCAAGGAGTGCAACTTCGCCATC
TCGCCCGCTGGCGTGGGGCGCTTTCGTGTGAATGCCTTTGTGCAGCAGGGCAAAGTGGGCATGGTGCTGCGGACAATTCC
GACCACGTTGCCTACGATTGATGGCCTGGGCGTGCCCCAGATCCTCAAGGAAGTGACCATGACCAAGCGCGGTCTGTGCA
TTCTGGTGGGTGCCACGGGCTCAGGCAAATCAACCACGCTGGCGGCCATGGTGGACTGGCGCAATGAGCAATCCTTCGGC
CACATCATCACCGTGGAAGACCCCGTGGAATTTGTGCACCCGCACAAGAACTGCGTGGTGACCCAGCGCGAAGTGGGGCT
GGATACCGAGAGCTGGGAAGCGGCGCTCAAAAACACGCTGCGCCAGGCCCCTGATGTGATTCTGATGGGCGAAATTCGCG
ACCGCGAAACCATGGAGCACGCAATTGCGTTCTCGGAAACCGGTCACCTGTGCCTGGCCACACTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGCCGCCCGCAGTTGCTGATGGATATCTCGTTGAACCT
GCGTGCCATCGTCTCCCAGCGCCTGATTCCCAAGCAGGATTCCAAGGGGCGCGTGGCCGCGATTGAGGTGATGCTGAACT
CTCCCCTGATTGCCGATCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAAATCATGAAAAAGAGCCGCAACGTGGGC
ATGCAGACCTTTGACCAGGCGCTGTTTGACCTGTTCGAGTCCAACCTGATTACCTATGAGGATGCACTGCGCAACGCGGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAACTCAACAGCCAGCGTGCGCGCACACAGGATCTGGCCGCGGGCACTGAGC
ACTTGACGATTGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.656

96.825

0.587
  pilU Acinetobacter baylyi ADP1

55.989

94.974

0.532
  pilU Vibrio cholerae strain A1552

52.617

96.032

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas aeruginosa PAK

43.917

89.153

0.392
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Pseudomonas stutzeri DSM 10701

42.433

89.153

0.378
  pilT Acinetobacter baylyi ADP1

41.742

88.095

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362