Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   JFT56_RS13705 Genome accession   NZ_CP066369
Coordinates   3149973..3151010 (-) Length   345 a.a.
NCBI ID   WP_007646255.1    Uniprot ID   F7RK78
Organism   Shewanella putrefaciens strain CGMCC-1.6515     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3144973..3156010
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFT56_RS13670 - 3145078..3145389 (+) 312 WP_198780612.1 hypothetical protein -
  JFT56_RS13675 yciH 3145545..3145874 (+) 330 WP_198780613.1 stress response translation initiation inhibitor YciH -
  JFT56_RS13680 - 3145968..3146531 (+) 564 WP_198780614.1 YqgE/AlgH family protein -
  JFT56_RS13685 ruvX 3146562..3146987 (+) 426 WP_198780615.1 Holliday junction resolvase RuvX -
  JFT56_RS13690 hemH 3147064..3148077 (-) 1014 WP_198780616.1 ferrochelatase -
  JFT56_RS13695 - 3148129..3148668 (-) 540 WP_198780617.1 glutathione peroxidase -
  JFT56_RS13700 pilU 3148851..3149963 (-) 1113 WP_198783580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JFT56_RS13705 pilT 3149973..3151010 (-) 1038 WP_007646255.1 type IV pilus twitching motility protein PilT Machinery gene
  JFT56_RS13710 - 3151046..3151744 (+) 699 WP_198780618.1 YggS family pyridoxal phosphate-dependent enzyme -
  JFT56_RS13715 proC 3151910..3152728 (+) 819 WP_198780619.1 pyrroline-5-carboxylate reductase -
  JFT56_RS13720 - 3152770..3153318 (+) 549 WP_198780620.1 YggT family protein -
  JFT56_RS13725 yggU 3153318..3153608 (+) 291 WP_198780621.1 DUF167 family protein YggU -
  JFT56_RS13730 - 3153700..3154134 (+) 435 WP_198780622.1 DUF4426 domain-containing protein -
  JFT56_RS13735 rdgB 3154260..3154877 (+) 618 WP_198780623.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38372.02 Da        Isoelectric Point: 6.9096

>NTDB_id=519487 JFT56_RS13705 WP_007646255.1 3149973..3151010(-) (pilT) [Shewanella putrefaciens strain CGMCC-1.6515]
MEITELLAFSVKHKASDLHLSAGISPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPNLAR
FRVNAFNQSRGAAAVFRTIPSEILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMVDYINENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAGEKDMVRTMLSESLQAVISQTLIKKIGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLEQCL
QNLVNRGLITREDAMSKSSNKQATF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=519487 JFT56_RS13705 WP_007646255.1 3149973..3151010(-) (pilT) [Shewanella putrefaciens strain CGMCC-1.6515]
ATGGAAATCACTGAGTTATTAGCCTTTAGTGTAAAACACAAAGCCTCGGATCTACACCTCTCTGCAGGGATATCTCCCAT
GATCCGTGTCGACGGTGAAGTGAGAAAAATTAACCTGCCGGCGCTCGATCATCAGGGCGTACACAGTTTAGTGTACGATA
TTATGAATGATAAACAGCGCAAGGACTATGAAGAGCATTTAGAAATCGATTTTTCCTTCGAGGTCCCTAATTTAGCCCGT
TTCCGGGTGAACGCATTTAATCAATCCCGTGGTGCGGCGGCGGTATTTCGTACCATTCCCAGTGAGATTTTGTCACTCGA
GCAGTTAGGTGCCCCCGAGATTTTTAAAAAAATCTCTAGTTTTCCCAGAGGGTTAGTCTTAGTTACTGGGCCTACAGGTT
CGGGTAAGAGTACCACACTTGCGGCCATGGTGGATTACATCAATGAAAACCGCCATGACCATATCTTAACGATCGAAGAT
CCAATCGAATTCGTTCACCAAAATAAGCAATGCCTGATCAACCAGCGGGAAGTACACCGTCATACCCACAGCTTTAATGC
CGCACTGCGCAGTGCACTGCGTGAGGACCCCGACGTGATCCTCGTCGGCGAGATGCGAGATCTTGAAACCATTCGCCTGG
CGATGACAGCGGCCGAAACCGGTCATTTAGTTTTCGGTACTTTGCACACCACCTCGGCAGCCAAAACCATCGACCGTGTG
GTCGACGTATTCCCCGCCGGTGAGAAAGACATGGTGCGCACTATGCTATCCGAGTCACTACAGGCGGTTATTTCACAAAC
GTTGATTAAAAAAATCGGTGGCGGGCGAGTCGCTGCCCACGAAATTATGATGGGCACACCTGCGATTCGTAACTTAATTC
GCGAAGATAAAGTGGCGCAAATGTACTCTGCAATTCAAACGGGTATGGCTCACGGGATGCAAACATTGGAACAATGTTTA
CAAAATCTAGTCAACCGTGGCCTCATCACCCGTGAGGACGCTATGTCTAAGAGTTCAAACAAACAAGCGACGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F7RK78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.883

99.42

0.794
  pilT Acinetobacter baumannii strain A118

79.883

99.42

0.794
  pilT Acinetobacter nosocomialis M2

79.883

99.42

0.794
  pilT Pseudomonas aeruginosa PAK

79.29

97.971

0.777
  pilT Acinetobacter baylyi ADP1

80.851

95.362

0.771
  pilT Pseudomonas stutzeri DSM 10701

77.193

99.13

0.765
  pilT Legionella pneumophila strain Lp02

75.367

98.841

0.745
  pilT Legionella pneumophila strain ERS1305867

75.367

98.841

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.681

0.71
  pilT Vibrio cholerae strain A1552

72.7

97.681

0.71
  pilT Neisseria meningitidis 8013

69.767

99.71

0.696
  pilT Neisseria gonorrhoeae MS11

69.477

99.71

0.693
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.558

98.261

0.487
  pilU Pseudomonas stutzeri DSM 10701

41.399

99.42

0.412
  pilU Vibrio cholerae strain A1552

41.071

97.391

0.4
  pilU Acinetobacter baylyi ADP1

39.466

97.681

0.386