Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   JFT56_RS13700 Genome accession   NZ_CP066369
Coordinates   3148851..3149963 (-) Length   370 a.a.
NCBI ID   WP_198783580.1    Uniprot ID   A0A2M7HSS2
Organism   Shewanella putrefaciens strain CGMCC-1.6515     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3143851..3154963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFT56_RS13665 - 3144338..3144898 (-) 561 WP_198780611.1 DUF4136 domain-containing protein -
  JFT56_RS13670 - 3145078..3145389 (+) 312 WP_198780612.1 hypothetical protein -
  JFT56_RS13675 yciH 3145545..3145874 (+) 330 WP_198780613.1 stress response translation initiation inhibitor YciH -
  JFT56_RS13680 - 3145968..3146531 (+) 564 WP_198780614.1 YqgE/AlgH family protein -
  JFT56_RS13685 ruvX 3146562..3146987 (+) 426 WP_198780615.1 Holliday junction resolvase RuvX -
  JFT56_RS13690 hemH 3147064..3148077 (-) 1014 WP_198780616.1 ferrochelatase -
  JFT56_RS13695 - 3148129..3148668 (-) 540 WP_198780617.1 glutathione peroxidase -
  JFT56_RS13700 pilU 3148851..3149963 (-) 1113 WP_198783580.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  JFT56_RS13705 pilT 3149973..3151010 (-) 1038 WP_007646255.1 type IV pilus twitching motility protein PilT Machinery gene
  JFT56_RS13710 - 3151046..3151744 (+) 699 WP_198780618.1 YggS family pyridoxal phosphate-dependent enzyme -
  JFT56_RS13715 proC 3151910..3152728 (+) 819 WP_198780619.1 pyrroline-5-carboxylate reductase -
  JFT56_RS13720 - 3152770..3153318 (+) 549 WP_198780620.1 YggT family protein -
  JFT56_RS13725 yggU 3153318..3153608 (+) 291 WP_198780621.1 DUF167 family protein YggU -
  JFT56_RS13730 - 3153700..3154134 (+) 435 WP_198780622.1 DUF4426 domain-containing protein -
  JFT56_RS13735 rdgB 3154260..3154877 (+) 618 WP_198780623.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41140.35 Da        Isoelectric Point: 6.5052

>NTDB_id=519486 JFT56_RS13700 WP_198783580.1 3148851..3149963(-) (pilU) [Shewanella putrefaciens strain CGMCC-1.6515]
MDVRPFLKVIVERKASDLFITAGFPPSAKIDGELRPLAESAFTPAQSLDFVESLMTEAQKKEFHTSRECNFAFAAKELGR
FRVSAFWQRESPGCVMRRIETKIPDVDDLKLPPILKDLVMSKRGLIIMVGGTGTGKSTSLAALVGYRNAHARGHILTIED
PVEFVHDHRKSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRTQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHQQLLFDLSLNLRGIVAQQLIPKVDGTGRRAAIEVLINTPRVASLIAKNELHLLKETMAKSNEQGMQTFDQA
LLKLYVDGEISYADALHHADSPNDLRLMIKLQNKEPNSSSFMEGVTLDMD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=519486 JFT56_RS13700 WP_198783580.1 3148851..3149963(-) (pilU) [Shewanella putrefaciens strain CGMCC-1.6515]
ATGGATGTACGTCCGTTTTTAAAAGTTATAGTGGAGCGAAAAGCCTCGGACTTATTTATTACCGCAGGCTTTCCGCCGAG
CGCGAAAATAGATGGTGAGTTACGCCCCCTCGCTGAGAGTGCATTTACGCCTGCGCAGTCCTTGGATTTTGTTGAATCCC
TGATGACAGAAGCGCAGAAGAAAGAGTTTCATACTAGTCGTGAATGTAACTTTGCCTTTGCAGCAAAGGAACTTGGGCGT
TTCCGGGTGAGTGCTTTTTGGCAGCGTGAATCCCCTGGTTGTGTTATGCGCCGAATCGAGACCAAAATTCCTGATGTGGA
TGATTTAAAACTCCCACCCATTCTTAAAGATCTGGTCATGAGTAAACGTGGGCTGATCATCATGGTGGGCGGTACTGGTA
CGGGTAAATCAACCTCCTTAGCCGCTTTAGTCGGTTATCGTAATGCCCATGCCCGCGGTCATATTTTGACTATTGAGGAT
CCTGTTGAATTTGTTCATGATCATAGAAAAAGTATCATTACCCAGCGTGAAGTTGGGATTGATACAGAATCTTTTGATGC
GGCGCTAAAAAGCTCGCTGCGTCAGGCGCCTGATGTGATCTTGATTGGTGAGATCCGTACTCAAGAAACCATGGAGTTCG
CTCTCTCGTTTGCCGAGACTGGTCACCTTTGTATGGCGACCTTGCATGCGAACAACGCTAACCAAGCGCTCGATCGGATC
ATGCATTTAGTGCCCGAGAGTAAACATCAACAGCTACTATTCGATTTATCCTTGAATTTACGTGGCATTGTGGCGCAGCA
ACTGATCCCTAAGGTGGATGGCACTGGGCGACGTGCCGCCATTGAGGTGCTTATTAACACCCCTAGGGTGGCGAGTTTAA
TTGCCAAAAATGAGCTGCATTTACTCAAAGAAACCATGGCCAAATCGAATGAGCAAGGTATGCAGACATTCGACCAAGCC
CTGTTAAAACTCTATGTCGATGGTGAGATTAGTTATGCCGATGCCCTGCACCATGCTGACTCACCTAACGATTTACGCTT
AATGATTAAGCTGCAAAATAAAGAACCTAACAGTTCAAGCTTTATGGAAGGCGTCACCCTAGACATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2M7HSS2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.838

100

0.6
  pilU Vibrio cholerae strain A1552

57.838

100

0.578
  pilU Acinetobacter baylyi ADP1

60.795

95.135

0.578
  pilT Vibrio cholerae strain A1552

42.727

89.189

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.727

89.189

0.381
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.58

93.243

0.378
  pilT Legionella pneumophila strain Lp02

40.785

89.459

0.365
  pilT Legionella pneumophila strain ERS1305867

40.785

89.459

0.365
  pilT Acinetobacter baumannii D1279779

39.181

92.432

0.362
  pilT Acinetobacter baumannii strain A118

39.181

92.432

0.362
  pilT Acinetobacter nosocomialis M2

39.181

92.432

0.362