Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ITG10_RS05445 Genome accession   NZ_CP066149
Coordinates   1256551..1257588 (-) Length   345 a.a.
NCBI ID   WP_017629549.1    Uniprot ID   -
Organism   Vibrio sp. ED004     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1251551..1262588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITG10_RS05415 (ITG10_05345) rsmE 1252283..1253014 (+) 732 WP_017629554.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ITG10_RS05420 (ITG10_05350) gshB 1253024..1253974 (+) 951 WP_017629553.1 glutathione synthase -
  ITG10_RS05425 (ITG10_05355) - 1254084..1254647 (+) 564 WP_017629552.1 YqgE/AlgH family protein -
  ITG10_RS05430 (ITG10_05360) ruvX 1254702..1255124 (+) 423 WP_017629551.1 Holliday junction resolvase RuvX -
  ITG10_RS05435 (ITG10_05365) - 1255223..1255366 (+) 144 WP_164913285.1 hypothetical protein -
  ITG10_RS05440 (ITG10_05370) pilU 1255427..1256536 (-) 1110 WP_017629550.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ITG10_RS05445 (ITG10_05375) pilT 1256551..1257588 (-) 1038 WP_017629549.1 type IV pilus twitching motility protein PilT Machinery gene
  ITG10_RS05450 (ITG10_05380) - 1257615..1258319 (+) 705 WP_248386743.1 YggS family pyridoxal phosphate-dependent enzyme -
  ITG10_RS05455 (ITG10_05385) proC 1258404..1259222 (+) 819 WP_017629547.1 pyrroline-5-carboxylate reductase -
  ITG10_RS05460 (ITG10_05390) - 1259280..1259837 (+) 558 WP_010435653.1 YggT family protein -
  ITG10_RS05465 (ITG10_05395) yggU 1259867..1260157 (+) 291 WP_009847703.1 DUF167 family protein YggU -
  ITG10_RS05470 (ITG10_05400) - 1260186..1260617 (+) 432 WP_016768173.1 DUF4426 domain-containing protein -
  ITG10_RS05475 (ITG10_05405) - 1260666..1261265 (+) 600 WP_017629546.1 XTP/dITP diphosphatase -
  ITG10_RS05480 (ITG10_05410) hemW 1261265..1262452 (+) 1188 WP_017629545.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38257.78 Da        Isoelectric Point: 6.0507

>NTDB_id=517871 ITG10_RS05445 WP_017629549.1 1256551..1257588(-) (pilT) [Vibrio sp. ED004]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQARGCSAVFRTIPVEIPTLDQLGAPEIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVERKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=517871 ITG10_RS05445 WP_017629549.1 1256551..1257588(-) (pilT) [Vibrio sp. ED004]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGTCATGCTGATGTGCATCGTTTAGTTTTTGAGA
TCATGAGCGATTCGCAGCGTGGTGAGTTTGAAGAAAAATTGGAAGTCGACTTCTCTTTTGAATTACCCAATGTTGGTCGT
TTCCGTGTGAATGCTTTTAACCAAGCTCGTGGTTGCTCAGCTGTCTTTCGAACAATCCCTGTAGAGATTCCAACTTTAGA
TCAGCTTGGTGCGCCAGAAATCTTCGAAAAGATTGCCAACTACGAAAAAGGTTTAGTGCTTGTCACAGGCCCTACTGGTT
CTGGTAAATCGACGACCCTTGCGGCGATGGTAGATTACGTAAACCGTAACCACAATAAGCATATCCTGACGATCGAAGAC
CCGATTGAATTCGTTCATACCAACAATAAATGCCTTGTTAACCAGCGTGAAGTTCATCGTGATACTCACAGCTTTAAAGC
GGCGCTGCGCAGTGCGTTACGTGAAGACCCAGACGTGATTCTTGTTGGTGAGCTTCGTGATCAAGAAACCATTAGCTTGG
CGCTAACCGCAGCGGAAACCGGCCACTTGGTTTTTGGTACTTTGCATACCAGTTCTGCTGCTAAAACCATCGACCGCATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGCTGTCTGAATCGTTACGTTCGGTCATTGCTCAGAA
GTTGTTGAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCTATTAGAAACTTGATTC
GTGAAGACAAGGTCGCGCAGATGTATTCGATCATTCAAACGGGCGCAGCACATGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGCTAATGGCTCAAGGCTTGGTTGACTCGGAAGAGGTCGAGAGAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

71.988

96.232

0.693
  pilT Acinetobacter baumannii strain A118

71.988

96.232

0.693
  pilT Acinetobacter nosocomialis M2

71.687

96.232

0.69
  pilT Pseudomonas aeruginosa PAK

68.824

98.551

0.678
  pilT Acinetobacter baylyi ADP1

70.909

95.652

0.678
  pilT Pseudomonas stutzeri DSM 10701

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.899

100

0.629
  pilT Neisseria gonorrhoeae MS11

62.609

100

0.626
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.44

98.841

0.499
  pilU Vibrio cholerae strain A1552

43.077

94.203

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.719

96.812

0.394
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371