NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	ITG10_RS05440	Genome accession	NZ_CP066149
Coordinates	1255427..1256536 (-)	Length	369 a.a.
NCBI ID	WP_017629550.1	Uniprot ID	-
Organism	Vibrio sp. ED004
Function	type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1250427..1261536

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ITG10_RS05405 (ITG10_05335)	-	1250746..1251246 (+)	501	WP_017629556.1	SprT family zinc-dependent metalloprotease	-
ITG10_RS05410 (ITG10_05340)	-	1251398..1252129 (+)	732	WP_017629555.1	endonuclease	-
ITG10_RS05415 (ITG10_05345)	rsmE	1252283..1253014 (+)	732	WP_017629554.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ITG10_RS05420 (ITG10_05350)	gshB	1253024..1253974 (+)	951	WP_017629553.1	glutathione synthase	-
ITG10_RS05425 (ITG10_05355)	-	1254084..1254647 (+)	564	WP_017629552.1	YqgE/AlgH family protein	-
ITG10_RS05430 (ITG10_05360)	ruvX	1254702..1255124 (+)	423	WP_017629551.1	Holliday junction resolvase RuvX	-
ITG10_RS05435 (ITG10_05365)	-	1255223..1255366 (+)	144	WP_164913285.1	hypothetical protein	-
ITG10_RS05440 (ITG10_05370)	pilU	1255427..1256536 (-)	1110	WP_017629550.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ITG10_RS05445 (ITG10_05375)	pilT	1256551..1257588 (-)	1038	WP_017629549.1	type IV pilus twitching motility protein PilT	Machinery gene
ITG10_RS05450 (ITG10_05380)	-	1257615..1258319 (+)	705	WP_248386743.1	YggS family pyridoxal phosphate-dependent enzyme	-
ITG10_RS05455 (ITG10_05385)	proC	1258404..1259222 (+)	819	WP_017629547.1	pyrroline-5-carboxylate reductase	-
ITG10_RS05460 (ITG10_05390)	-	1259280..1259837 (+)	558	WP_010435653.1	YggT family protein	-
ITG10_RS05465 (ITG10_05395)	yggU	1259867..1260157 (+)	291	WP_009847703.1	DUF167 family protein YggU	-
ITG10_RS05470 (ITG10_05400)	-	1260186..1260617 (+)	432	WP_016768173.1	DUF4426 domain-containing protein	-
ITG10_RS05475 (ITG10_05405)	-	1260666..1261265 (+)	600	WP_017629546.1	XTP/dITP diphosphatase	-

Sequence

Protein

Download Length: 369 a.a. Molecular weight: 41353.17 Da Isoelectric Point: 5.6716

                                >NTDB_id=517870 ITG10_RS05440 WP_017629550.1 1255427..1256536(-) (pilU) [Vibrio sp. ED004]

MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGDKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVREFGRFR

VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI

EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH

LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEVGMQTFDQALY

DLVEAGKISEEDAFHSADSANDLRLMLKTRRGDDDYGTGALAGVKIDMG

Nucleotide

Download Length: 1110 bp

                                >NTDB_id=517870 ITG10_RS05440 WP_017629550.1 1255427..1256536(-) (pilU) [Vibrio sp. ED004]

ATGGAATTGAATCAAATCCTTGAAGGGATGCTGTCTCAAAAGGCATCGGATCTTTATATAACCGTTGACGCGCCTATCCT

GTTTCGTGTTGATGGTGAATTACGACCTCAAGGCGATAAGCTGAATGCGGCTCAGGTTGCTCAATTGCTTGATGCGATGA

TGGATCAAGACCGACGTGATGAATATCAGCAAACACGTGAGGCAAACTTTGCCATTGTGCGTGAGTTTGGTCGTTTTCGT

GTCAGTGCATTCTTCCAACGAGAGCTACCTGGAGCGGTAATTCGACGCATCGAGACTAACATCCCAACCTTTGAGCAATT

AAAGCTTCCTGATGTATTACAAGACCTGTCAATCGCTAAGCGTGGGCTTGTGCTGGTGGTTGGTGCGACTGGCTCTGGTA

AATCGACCTCGATGGCTGCAATGACAGGTTATCGCAATACCAATCGTTCAGGGCACATCTTAACGGTTGAAGACCCAATT

GAGTTCGTCCACGAGCATAAAAAATGTATCGTGACTCAGCGTGAGGTAGGGCTCGACACTGAGAGCTATGAAGTCGCACT

TAAGAACTCTTTGCGTCAAGCGCCAGACATGATCTTGATTGGCGAAATCCGTAGCCGTGAAACTATGGAATACGCGATGA

CCTTTGCTGAAACGGGTCACTTGTGTATGGCTACACTGCATGCGAATAACGCCAACCAAGCGCTAGAACGTATTCTTCAC

TTGGTGCCGAAAGAACAGAAAGAGCAGTTCCTGTTTGACCTGTCGATGAATCTGCGTGGTGTGGTCGCTCAACAGTTAAT

TCGAGATAAGAATGGCAGCGGTCGTCATGGTGTGTTTGAGATTTTACTCAACAGCCCCCGAGTGTCTGACTTAATTCGTC

GCGGTGAGCTGCACGAGCTAAAAGCAACAATGGCGAAATCGAAAGAGGTTGGTATGCAGACCTTTGACCAAGCTTTGTAT

GATTTAGTTGAAGCAGGCAAGATCAGCGAAGAGGATGCGTTCCACAGTGCTGACTCTGCTAATGACTTGCGCTTGATGCT

GAAAACCAGACGAGGCGATGATGACTATGGGACTGGTGCTTTGGCTGGTGTGAAGATAGATATGGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Vibrio cholerae strain A1552	84.239	99.729	0.84
pilU	Pseudomonas stutzeri DSM 10701	56.065	100	0.564
pilU	Acinetobacter baylyi ADP1	54.986	95.122	0.523
pilT	Acinetobacter baumannii D1279779	42.353	92.141	0.39
pilT	Acinetobacter nosocomialis M2	42.353	92.141	0.39
pilT	Acinetobacter baumannii strain A118	42.353	92.141	0.39
pilT	Legionella pneumophila strain ERS1305867	42.183	91.87	0.388
pilT	Legionella pneumophila strain Lp02	42.183	91.87	0.388
pilT	Acinetobacter baylyi ADP1	42.169	89.973	0.379
pilT	Pseudomonas aeruginosa PAK	41.791	90.786	0.379
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.593	87.805	0.374
pilT	Pseudomonas stutzeri DSM 10701	40.299	90.786	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.419	90.515	0.366
pilT	Vibrio cholerae strain A1552	40.419	90.515	0.366
pilT	Neisseria meningitidis 8013	38.841	93.496	0.363
pilT	Neisseria gonorrhoeae MS11	38.841	93.496	0.363

Detailed information NTG0517870