Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DESTI_RS05865 Genome accession   NC_018025
Coordinates   1394314..1395417 (-) Length   367 a.a.
NCBI ID   WP_014809041.1    Uniprot ID   I4C2U7
Organism   Desulfomonile tiedjei DSM 6799     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1389314..1400417
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DESTI_RS05850 (Desti_1172) - 1391547..1392251 (-) 705 WP_014809038.1 hypothetical protein -
  DESTI_RS05855 (Desti_1173) - 1392255..1393532 (-) 1278 WP_014809039.1 sulfite exporter TauE/SafE family protein -
  DESTI_RS05860 (Desti_1174) - 1393787..1394206 (-) 420 WP_014809040.1 response regulator -
  DESTI_RS05865 (Desti_1175) pilT 1394314..1395417 (-) 1104 WP_014809041.1 type IV pilus twitching motility protein PilT Machinery gene
  DESTI_RS05870 (Desti_1176) recA 1395407..1396510 (-) 1104 WP_014809042.1 recombinase RecA Machinery gene
  DESTI_RS05875 (Desti_1177) - 1396877..1397815 (+) 939 WP_014809043.1 DUF1848 domain-containing protein -
  DESTI_RS05880 (Desti_1178) - 1397818..1398957 (+) 1140 WP_041285996.1 energy transducer TonB -
  DESTI_RS05885 (Desti_1179) gcvH 1399348..1399740 (-) 393 WP_014809045.1 glycine cleavage system protein GcvH -
  DESTI_RS05890 (Desti_1180) - 1399990..1400256 (+) 267 WP_014809046.1 hypothetical protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40856.12 Da        Isoelectric Point: 6.7732

>NTDB_id=51738 DESTI_RS05865 WP_014809041.1 1394314..1395417(-) (pilT) [Desulfomonile tiedjei DSM 6799]
MDLDECLRKAIESGASDIHLKVPLPPVTRINGVLAPMRGEDRLSPDEVRRIALSIMNERQREIFTKTKELDMAYSVSKLA
RFRVNVFSQRDSIAMVFRTIPSEPPMLKDLSLPPVIEKIAMEHRGLVLVTGTTGSGKSTTLAAMINHINSFRNCHLITIE
DPIEFLHRDRKSIINQREVGQDTLSFQSALRAALREDPDVILVGEMRDLETIEIAMLAAETGHLVFSTLHTLDATETINR
IISAFPIEHQGSIRLQLAGILKGVISQRLLSKADGKGRVPAVEVLVSTARIRECIGDERRIREIRDAIAEGHISYGMQTF
DQSLMQLYKKNLVTYETALGAASNPDDFALFVRGISGTSEGKWDMSA

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=51738 DESTI_RS05865 WP_014809041.1 1394314..1395417(-) (pilT) [Desulfomonile tiedjei DSM 6799]
ATGGATCTTGATGAATGTCTGAGAAAAGCAATTGAAAGCGGAGCTTCCGATATCCACTTGAAAGTTCCTCTTCCGCCGGT
AACCCGGATCAACGGCGTACTCGCACCCATGAGAGGTGAGGACAGGCTCAGTCCTGATGAAGTTCGTCGAATCGCTCTGT
CCATTATGAACGAGCGCCAACGCGAAATCTTCACCAAGACGAAAGAGTTGGACATGGCTTACAGTGTTTCAAAACTGGCC
AGGTTCAGGGTAAACGTGTTCTCCCAGCGGGATTCCATTGCCATGGTGTTCAGGACGATTCCCTCTGAACCTCCCATGCT
GAAAGATCTCAGTCTTCCGCCGGTCATCGAGAAAATTGCAATGGAACACAGGGGCTTAGTCCTCGTCACCGGAACAACCG
GTTCAGGAAAATCGACAACCCTCGCTGCAATGATCAACCACATCAACTCATTCAGGAACTGTCATCTTATAACGATCGAA
GATCCGATAGAATTCCTTCACAGAGACCGCAAAAGCATTATCAATCAACGCGAGGTCGGTCAGGATACGCTTTCGTTCCA
GAGCGCATTGAGGGCTGCATTGCGTGAAGATCCCGACGTCATCCTCGTGGGCGAAATGCGGGATCTCGAGACAATCGAAA
TAGCCATGTTGGCGGCAGAGACCGGGCATCTTGTCTTCAGCACCCTGCATACTCTGGATGCGACCGAAACCATCAACCGA
ATCATCTCTGCATTCCCTATCGAGCACCAGGGTTCGATCCGGCTTCAGTTAGCAGGCATTCTCAAAGGGGTTATATCCCA
GCGCCTTCTCTCAAAGGCGGATGGAAAAGGCCGTGTACCTGCCGTTGAAGTCCTGGTATCTACAGCCCGCATTCGGGAGT
GCATCGGAGACGAGCGCAGAATCAGAGAAATTCGAGATGCAATTGCCGAAGGTCACATCAGTTACGGAATGCAAACCTTC
GATCAATCGCTCATGCAATTGTACAAGAAGAATCTGGTCACGTACGAGACGGCTCTCGGCGCAGCCAGTAACCCGGACGA
TTTTGCCCTATTCGTAAGAGGTATCAGCGGCACGTCAGAAGGCAAATGGGATATGAGCGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4C2U7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

50.142

96.185

0.482
  pilT Legionella pneumophila strain ERS1305867

50.142

96.185

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.76

98.91

0.482
  pilT Acinetobacter baylyi ADP1

48.563

94.823

0.46
  pilT Pseudomonas aeruginosa PAK

47.309

96.185

0.455
  pilT Neisseria meningitidis 8013

47.429

95.368

0.452
  pilT Pseudomonas stutzeri DSM 10701

47.701

94.823

0.452
  pilT Acinetobacter nosocomialis M2

47.701

94.823

0.452
  pilT Acinetobacter baumannii D1279779

47.701

94.823

0.452
  pilT Acinetobacter baumannii strain A118

47.701

94.823

0.452
  pilT Neisseria gonorrhoeae MS11

47.143

95.368

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.447

90.736

0.431
  pilT Vibrio cholerae strain A1552

47.447

90.736

0.431
  pilU Acinetobacter baylyi ADP1

41.854

97.003

0.406
  pilU Vibrio cholerae strain A1552

39.946

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

41.643

96.185

0.401

Multiple sequence alignment