Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THIVI_RS16075 Genome accession   NC_018012
Coordinates   3628551..3629588 (-) Length   345 a.a.
NCBI ID   WP_014779597.1    Uniprot ID   I3YDX2
Organism   Thiocystis violascens DSM 198     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3623551..3634588
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIVI_RS16050 (Thivi_3312) ruvX 3624017..3624430 (+) 414 WP_014779592.1 Holliday junction resolvase RuvX -
  THIVI_RS16055 (Thivi_3313) pyrR 3624417..3625070 (+) 654 WP_014779593.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  THIVI_RS16060 (Thivi_3314) - 3625067..3626065 (+) 999 WP_014779594.1 aspartate carbamoyltransferase catalytic subunit -
  THIVI_RS16065 (Thivi_3315) - 3626058..3627350 (+) 1293 WP_014779595.1 dihydroorotase -
  THIVI_RS16070 (Thivi_3316) pilU 3627394..3628551 (-) 1158 WP_014779596.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THIVI_RS16075 (Thivi_3317) pilT 3628551..3629588 (-) 1038 WP_014779597.1 type IV pilus twitching motility protein PilT Machinery gene
  THIVI_RS16080 (Thivi_3318) - 3629694..3630491 (-) 798 WP_014779598.1 Uma2 family endonuclease -
  THIVI_RS16085 (Thivi_3319) purE 3630494..3631015 (-) 522 WP_014779599.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  THIVI_RS16090 (Thivi_3320) purK 3631012..3632166 (-) 1155 WP_014779600.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  THIVI_RS16095 (Thivi_3321) - 3632222..3632638 (-) 417 WP_014779601.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38276.11 Da        Isoelectric Point: 7.6495

>NTDB_id=51658 THIVI_RS16075 WP_014779597.1 3628551..3629588(-) (pilT) [Thiocystis violascens DSM 198]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDMRRINVPPLEHRSVHSLVYDIMNDKQRKDYEEFLETDFSFEIPGVAR
FRVNAFNQKRGAGAVFRTIPSKVLTLEDLKAPASFKDIVDVPRGLVLVTGPTGSGKSTTLAAMVDHLNDNKYEHILTIED
PIEFVHTSKKCLVNQREVHRDTLGFSEALRSALRQDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMVRAMLSESLRSVIAQTLLKKTGGGRIAAHEIMIGTPAIRNLIREAKVAQMYSAIQTGQAHGMQTLDQNL
KELMQKGLIARKDARAKAQNKDDFM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=51658 THIVI_RS16075 WP_014779597.1 3628551..3629588(-) (pilT) [Thiocystis violascens DSM 198]
GTGGATATCGCCGAACTACTTGCCTTCAGTGTCAAGAACAATGCCTCCGACCTGCATCTGTCGGCCGGGCTTCCCCCCAT
GATTCGTGTCGACGGCGATATGCGCCGGATCAATGTTCCGCCCCTGGAACATCGTTCGGTGCATTCGTTGGTCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTGGAGACCGACTTTTCGTTCGAGATCCCGGGCGTGGCGCGT
TTTCGGGTCAATGCCTTCAACCAGAAGCGTGGCGCTGGCGCGGTCTTTCGGACCATTCCCTCGAAGGTGCTGACGCTGGA
GGATCTGAAGGCTCCGGCGAGTTTCAAGGACATCGTCGATGTCCCGCGCGGTCTGGTGCTGGTGACCGGGCCGACCGGCT
CCGGCAAATCGACCACCCTGGCGGCGATGGTGGATCATCTCAACGACAATAAATACGAACACATCCTCACCATCGAGGAT
CCGATCGAGTTCGTGCATACCAGCAAAAAGTGTCTGGTCAATCAGCGCGAGGTCCATCGCGATACCCTCGGCTTCAGCGA
GGCGCTACGCTCCGCCCTGCGCCAGGATCCGGACATCATCCTGGTCGGCGAAATGCGCGACCTGGAGACCATCCGGCTCG
CGCTCACCGCCGCCGAGACCGGCCATTTGGTGTTCGGCACCCTGCATACCACCTCGGCGGCCAAGACCATTGACCGCATC
ATCGACGTCTTTCCCGCCGCCGAGAAGGACATGGTCCGCGCCATGCTCTCGGAATCGCTGCGCTCGGTCATCGCCCAGAC
ACTGCTGAAAAAGACCGGGGGCGGCCGGATCGCGGCCCACGAGATCATGATCGGCACGCCCGCCATCCGCAACCTGATCC
GCGAGGCCAAGGTCGCTCAAATGTATTCGGCGATCCAGACCGGTCAGGCGCACGGTATGCAGACCCTGGATCAGAATCTG
AAAGAGCTGATGCAGAAGGGCCTGATCGCGCGCAAGGATGCCCGCGCGAAGGCCCAGAACAAGGACGATTTCATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3YDX2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Pseudomonas stutzeri DSM 10701

81.686

99.71

0.814
  pilT Acinetobacter nosocomialis M2

80.87

100

0.809
  pilT Acinetobacter baumannii D1279779

80.87

100

0.809
  pilT Acinetobacter baumannii strain A118

80.87

100

0.809
  pilT Acinetobacter baylyi ADP1

79.07

99.71

0.788
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.206

98.261

0.69
  pilT Vibrio cholerae strain A1552

70.206

98.261

0.69
  pilT Neisseria meningitidis 8013

67.536

100

0.675
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Pseudomonas stutzeri DSM 10701

42.029

100

0.42
  pilU Vibrio cholerae strain A1552

40.29

100

0.403
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment