Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   THIVI_RS16070 Genome accession   NC_018012
Coordinates   3627394..3628551 (-) Length   385 a.a.
NCBI ID   WP_014779596.1    Uniprot ID   I3YDX1
Organism   Thiocystis violascens DSM 198     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3622394..3633551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THIVI_RS16045 (Thivi_3311) - 3623432..3623998 (+) 567 WP_014779591.1 YqgE/AlgH family protein -
  THIVI_RS16050 (Thivi_3312) ruvX 3624017..3624430 (+) 414 WP_014779592.1 Holliday junction resolvase RuvX -
  THIVI_RS16055 (Thivi_3313) pyrR 3624417..3625070 (+) 654 WP_014779593.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  THIVI_RS16060 (Thivi_3314) - 3625067..3626065 (+) 999 WP_014779594.1 aspartate carbamoyltransferase catalytic subunit -
  THIVI_RS16065 (Thivi_3315) - 3626058..3627350 (+) 1293 WP_014779595.1 dihydroorotase -
  THIVI_RS16070 (Thivi_3316) pilU 3627394..3628551 (-) 1158 WP_014779596.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THIVI_RS16075 (Thivi_3317) pilT 3628551..3629588 (-) 1038 WP_014779597.1 type IV pilus twitching motility protein PilT Machinery gene
  THIVI_RS16080 (Thivi_3318) - 3629694..3630491 (-) 798 WP_014779598.1 Uma2 family endonuclease -
  THIVI_RS16085 (Thivi_3319) purE 3630494..3631015 (-) 522 WP_014779599.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  THIVI_RS16090 (Thivi_3320) purK 3631012..3632166 (-) 1155 WP_014779600.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  THIVI_RS16095 (Thivi_3321) - 3632222..3632638 (-) 417 WP_014779601.1 hypothetical protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42899.22 Da        Isoelectric Point: 6.0970

>NTDB_id=51657 THIVI_RS16070 WP_014779596.1 3627394..3628551(-) (pilU) [Thiocystis violascens DSM 198]
MDLKRALEQLLGALDEKKGSDLFITTGWPPSIKIDGAIYPAAKQPLTADQARGMVYEIMNERQRAEFDDTKECQFAIDRS
PLGRFRVSAFVQRDSVGMVLRRIESRIPTLEELKLPSILRDLGMVKRGLVIFVGATGTGKSTSLAALVGYRNRKSSGHII
TVEDPIEFVHEHDGCIITQREVGVDTESFEVALRNTLRQAPDVILIGEIRTRATMEYAITFAETGHLVLATLHANNANQA
LDRVINFFPEDSRDQMLLDLSLNLKAIVAQQLVPRKSGQGRRAVVEILLNTPLAADLIRKGEVSKLKELMKKSGEQGMIT
FDQALFNLYEEGEISYEDAIRYADSANEVRLIIKLQGGTAQRAATDRTIDGITLVQEEERKPPPR

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=51657 THIVI_RS16070 WP_014779596.1 3627394..3628551(-) (pilU) [Thiocystis violascens DSM 198]
ATGGACCTGAAACGCGCGCTGGAACAACTGCTTGGCGCACTGGACGAAAAGAAGGGTTCCGACCTCTTCATCACGACCGG
CTGGCCGCCCAGTATCAAGATCGATGGCGCGATCTACCCCGCCGCCAAGCAGCCGCTCACCGCCGATCAGGCGCGCGGCA
TGGTCTATGAAATCATGAACGAGCGCCAGCGCGCCGAGTTCGACGACACCAAGGAGTGCCAATTCGCGATCGACCGCTCG
CCGCTCGGTCGCTTCCGCGTCAGCGCCTTCGTCCAGCGCGATTCGGTCGGGATGGTGCTGCGGCGCATCGAGTCCCGGAT
CCCGACCCTGGAAGAACTCAAACTGCCCTCGATTCTGCGCGATCTGGGCATGGTTAAACGCGGCCTCGTGATTTTCGTCG
GCGCGACCGGTACCGGCAAATCCACCTCGCTGGCGGCCCTCGTCGGCTATCGCAATCGCAAGAGCAGCGGACACATCATC
ACCGTCGAGGATCCGATCGAGTTCGTGCATGAACACGACGGCTGCATCATCACACAGCGCGAGGTGGGGGTAGATACCGA
ATCCTTCGAGGTCGCGCTGCGCAATACCCTGCGCCAGGCCCCGGATGTCATCCTGATCGGCGAGATCCGCACCCGGGCGA
CCATGGAATACGCCATCACCTTCGCCGAAACCGGCCATCTGGTGCTGGCCACCCTGCACGCCAACAATGCCAATCAGGCG
TTGGACCGCGTCATCAACTTCTTTCCGGAAGACTCCCGCGATCAGATGCTGTTGGATCTGTCGCTCAACCTCAAGGCCAT
CGTCGCCCAGCAGTTGGTGCCCCGCAAGAGCGGCCAGGGGCGGCGCGCGGTGGTCGAAATCCTGCTCAACACGCCGCTGG
CCGCCGACCTGATCCGCAAGGGCGAGGTGAGCAAACTCAAGGAGTTGATGAAGAAGTCCGGCGAGCAGGGCATGATCACC
TTCGACCAGGCGCTCTTCAATCTCTACGAGGAGGGCGAGATCAGCTACGAGGACGCGATCCGCTACGCCGATTCGGCCAA
CGAGGTGCGACTCATCATCAAATTACAGGGTGGCACCGCGCAACGGGCCGCGACCGATCGCACCATCGACGGTATCACGC
TGGTGCAGGAGGAAGAGCGGAAGCCGCCGCCGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3YDX1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.195

90.649

0.618
  pilU Acinetobacter baylyi ADP1

62.248

90.13

0.561
  pilU Vibrio cholerae strain A1552

57.955

91.429

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.293

85.195

0.369
  pilT Acinetobacter baylyi ADP1

41.593

88.052

0.366
  pilT Legionella pneumophila strain Lp02

41.742

86.494

0.361
  pilT Legionella pneumophila strain ERS1305867

41.742

86.494

0.361

Multiple sequence alignment