Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I8T81_RS14550 Genome accession   NZ_CP065820
Coordinates   3172871..3173908 (+) Length   345 a.a.
NCBI ID   WP_025469189.1    Uniprot ID   -
Organism   Acinetobacter seifertii strain S21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3167871..3178908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I8T81_RS14540 (I8T81_14540) - 3171103..3171945 (-) 843 WP_068933939.1 undecaprenyl-diphosphate phosphatase -
  I8T81_RS14545 (I8T81_14545) - 3172052..3172744 (-) 693 WP_198071542.1 YggS family pyridoxal phosphate-dependent enzyme -
  I8T81_RS14550 (I8T81_14550) pilT 3172871..3173908 (+) 1038 WP_025469189.1 type IV pilus twitching motility protein PilT Machinery gene
  I8T81_RS14555 (I8T81_14555) pilU 3173936..3175054 (+) 1119 WP_004703285.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I8T81_RS14560 (I8T81_14560) fur 3175124..3175561 (-) 438 WP_004703287.1 ferric iron uptake transcriptional regulator -
  I8T81_RS14565 (I8T81_14565) - 3175673..3176071 (+) 399 WP_004703289.1 outer membrane protein assembly factor BamE -
  I8T81_RS14570 (I8T81_14570) - 3176127..3176450 (-) 324 WP_077165706.1 RnfH family protein -
  I8T81_RS14575 (I8T81_14575) - 3176450..3177517 (-) 1068 WP_039254389.1 membrane protein -
  I8T81_RS14580 (I8T81_14580) - 3177670..3178125 (-) 456 WP_004703295.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38387.01 Da        Isoelectric Point: 6.6653

>NTDB_id=514750 I8T81_RS14550 WP_025469189.1 3172871..3173908(+) (pilT) [Acinetobacter seifertii strain S21]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KALVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=514750 I8T81_RS14550 WP_025469189.1 3172871..3173908(+) (pilT) [Acinetobacter seifertii strain S21]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCTTCCGATTTACATTTATCTGCTGGCATGCCACCAAT
GATTCGTGTCGATGGTGAAGTTCGTCGTATTAACTTACCAGCCTTAGAACATAAAGATGTCCACCGCCTTGTGTACGACA
TCATGAATGATAAACAGCGCCGTGATTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCACGT
TTCCGTGTGAACGCATTTAACCAGAACCGTGGTGCTGGCGCAGTATTTCGTACCATTCCATCTAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGACATTTGTGACTATCCTCGCGGGATTGTCCTAGTTACAGGTCCAACAGGTT
CGGGTAAATCAACCACGCTTGCAGCGATGATGGACTATATTAATGAAAATCGTTATGACCATATTTTAACGGTTGAAGAC
CCAATCGAATTTGTACACCAGTCAAAAAAATGCCTCATTAACCAGCGTGAAGTACATCGTGATACACATGGCTTTAATGA
AGCACTTCGCTCAGCATTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACGATTCGTTTGG
CCCTTACAGCTGCCGAAACAGGTCACTTAGTATTTGGTACGCTTCATACCACATCTGCCGCTAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTACGTGCCATGCTGTCTGAATCTTTGCAAGCCGTTATTTCGCAAAC
TCTGCTTAAAAAGAACGGCGGCGGTCGTGTGGCAGCACATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATCC
GTGAAAACAAAGTCGCACAAATGTATTCAGCTATTCAAACTGGTGCTAACTATGGCATGACAACGCTTGACCAAAGCTTA
AAAGCTTTAGTGTCACGTGGTGTAATTAGCCCACAAACTGCACGTACTGCGGCAAAACAACCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

98.841

100

0.988
  pilT Acinetobacter baumannii strain A118

98.841

100

0.988
  pilT Acinetobacter baylyi ADP1

88.081

99.71

0.878
  pilT Pseudomonas aeruginosa PAK

83.43

99.71

0.832
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388