Detailed information NTG0514751

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I8T81_RS14555
• Genome accession: NZ_CP065820
• Coordinates: 3173936..3175054 (+)
• Length: 372 a.a.
• NCBI ID: WP_004703285.1
• Uniprot ID: N8S8R6
• Organism: Acinetobacter seifertii strain S21
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3168936..3180054

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I8T81_RS14540 (I8T81_14540)	-	3171103..3171945 (-)	843	WP_068933939.1	undecaprenyl-diphosphate phosphatase	-
I8T81_RS14545 (I8T81_14545)	-	3172052..3172744 (-)	693	WP_198071542.1	YggS family pyridoxal phosphate-dependent enzyme	-
I8T81_RS14550 (I8T81_14550)	pilT	3172871..3173908 (+)	1038	WP_025469189.1	type IV pilus twitching motility protein PilT	Machinery gene
I8T81_RS14555 (I8T81_14555)	pilU	3173936..3175054 (+)	1119	WP_004703285.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
I8T81_RS14560 (I8T81_14560)	fur	3175124..3175561 (-)	438	WP_004703287.1	ferric iron uptake transcriptional regulator	-
I8T81_RS14565 (I8T81_14565)	-	3175673..3176071 (+)	399	WP_004703289.1	outer membrane protein assembly factor BamE	-
I8T81_RS14570 (I8T81_14570)	-	3176127..3176450 (-)	324	WP_077165706.1	RnfH family protein	-
I8T81_RS14575 (I8T81_14575)	-	3176450..3177517 (-)	1068	WP_039254389.1	membrane protein	-
I8T81_RS14580 (I8T81_14580)	-	3177670..3178125 (-)	456	WP_004703295.1	bacteriohemerythrin	-
I8T81_RS14585 (I8T81_14585)	-	3178376..3179224 (-)	849	WP_019456827.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41966.15 Da
• Isoelectric Point: 6.6944

>NTDB_id=514751 I8T81_RS14555 WP_004703285.1 3173936..3175054(+) (pilU) [Acinetobacter seifertii strain S21]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPSVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKTGQITYKDALKHADSPNDLRLTIKLANEGPDQIAEEKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=514751 I8T81_RS14555 WP_004703285.1 3173936..3175054(+) (pilU) [Acinetobacter seifertii strain S21]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGTGTTGCACCATC
TATGAAAATTAATGGTCAAATTGTTCCCATTTCAAAGAATAGCCTTTCTGGTGAAGTAATTGGACAGCTCTTACATTCGA
TTATGAGTGAGAAGCAGCGTAAAGAATTTGCGGACACTCGTGAATGTAACTTTGCCATTATGAATCGTGACAAAACGGCA
CGTTTTCGTGTGAGTGCTTTTCAGCAACGCGATATGCCAGGCATGGTTCTACGTCGAATTGAAACTAAAATTCCTTCGAT
TGATGACTTACAGTTACCCTCTGTGCTTAAAGATTTATCAATGACCAAACGCGGCATTATTATTTTTGTAGGCGCAACAG
GTACTGGTAAATCGACTTCGTTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAAGGTCATATCATTACTATTGAA
GACCCGATTGAGTTTATTCACGAACATGCGGGCTGTATTATTACTCAGCGTGAGGTCGGTATTGATACTGACTCGTTTGA
AATTGCTTTGAAAAATACTTTACGACAAGCACCCGATGTCATCTTAATTGGTGAGATCCGTTCTCGTGAAGTAATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCCACAATGCACGCCAACAACGCTAACCAAGCACTCGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTATATGGACTTATCTCTCAACTTAAAGGCCATGATTGCTCA
GCAACTTATTCCAACGCCAGATGGAAATTCACGCCGTGCAGCGATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGACTTAATGAAACGCTCACGTGAACTCGGTATGCAAACCTTTGACCAG
GCTTTATTTGACTTATATAAAACAGGTCAAATTACCTATAAAGATGCGCTTAAACATGCCGACTCACCAAACGATTTACG
TTTGACGATTAAGCTTGCAAATGAAGGTCCAGATCAAATTGCAGAAGAAAAACAGCATTTGACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (36-270)

3D structure

• AlphaFold DB: N8S8R6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.132	100	0.763
pilU	Pseudomonas stutzeri DSM 10701	68.478	98.925	0.677
pilU	Vibrio cholerae strain A1552	55.271	94.355	0.522
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.972	95.43	0.401