Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6G27_RS04925 Genome accession   NZ_CP065751
Coordinates   1103683..1104732 (-) Length   349 a.a.
NCBI ID   WP_062331666.1    Uniprot ID   A0A378Q9P8
Organism   Moraxella osloensis strain FDAARGOS_870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1098683..1109732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G27_RS04910 (I6G27_04910) ribF 1100683..1101708 (-) 1026 WP_062331675.1 riboflavin biosynthesis protein RibF -
  I6G27_RS04915 (I6G27_04915) - 1101834..1102370 (+) 537 WP_062331672.1 GNAT family N-acetyltransferase -
  I6G27_RS04920 (I6G27_04920) pilU 1102442..1103593 (-) 1152 WP_062331669.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6G27_RS04925 (I6G27_04925) pilT 1103683..1104732 (-) 1050 WP_062331666.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G27_RS04930 (I6G27_04930) - 1105005..1105730 (+) 726 WP_062331663.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6G27_RS04935 (I6G27_04935) - 1106156..1106680 (+) 525 WP_062331660.1 C40 family peptidase -
  I6G27_RS04940 (I6G27_04940) - 1107018..1108367 (+) 1350 WP_062331657.1 diguanylate cyclase -
  I6G27_RS04945 (I6G27_04945) truD 1108362..1109465 (-) 1104 WP_062331655.1 tRNA pseudouridine(13) synthase TruD -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38861.59 Da        Isoelectric Point: 6.9911

>NTDB_id=513970 I6G27_RS04925 WP_062331666.1 1103683..1104732(-) (pilT) [Moraxella osloensis strain FDAARGOS_870]
MAKELSIGDLLRFAFKNKASDLHISAGLPPMIRVDGEITKINMPPLEHQKVHSLIYDIMNDKQRADYEEFFETDFSFEIP
NVARFRVNAFNQNRGAGAVFRTIPSEVLTMEQLGMGPIFEKISNYKRGVVLVTGPTGSGKSTTLAAMINYINESRKEHIL
TIEDPIEFVHQSKKSLINQREVHRDTLSFDAALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKT
IDRVVDVFPAAEKDMIRAMLSESLQAVISQSLLKRQTGGRVAAHEIMIGTPAIRNLIRENKVAQMYSAIQTSAGDGMTTL
DQSLRELVNKGVISKESARQYAKQPESFM

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=513970 I6G27_RS04925 WP_062331666.1 1103683..1104732(-) (pilT) [Moraxella osloensis strain FDAARGOS_870]
ATGGCAAAGGAATTATCGATTGGCGATTTGTTGCGTTTTGCATTCAAAAATAAAGCCTCTGACTTGCACATTTCAGCAGG
CTTACCGCCGATGATTCGGGTAGATGGTGAAATCACCAAAATCAACATGCCGCCGCTTGAGCATCAAAAAGTGCATAGTT
TGATTTATGACATCATGAACGACAAACAACGTGCCGATTATGAAGAGTTTTTTGAAACCGACTTTTCGTTTGAAATTCCC
AATGTGGCGCGCTTTCGTGTCAATGCCTTTAACCAAAACCGTGGCGCAGGTGCGGTATTTCGTACCATTCCGTCTGAGGT
CTTGACGATGGAGCAGCTTGGGATGGGACCGATTTTTGAGAAAATCTCTAATTACAAACGCGGCGTGGTACTGGTCACCG
GCCCAACGGGTTCGGGCAAGTCAACTACCCTTGCGGCGATGATTAACTATATCAATGAAAGCCGTAAAGAACATATTTTA
ACCATCGAAGACCCGATTGAATTTGTGCATCAATCAAAAAAATCCCTCATCAACCAACGGGAAGTCCACCGTGATACCTT
AAGCTTTGACGCCGCGCTGCGCTCTGCCCTACGTGAAGACCCTGACGTTATTCTTGTCGGTGAGATGCGTGACCTTGAGA
CCATCCGCTTGGCATTGACGGCTGCTGAAACAGGGCACTTGGTATTTGGCACATTGCATACCTCATCTGCTGCCAAGACC
ATTGACCGTGTGGTCGACGTGTTCCCCGCGGCTGAAAAAGACATGATTCGTGCGATGTTATCTGAGTCACTGCAAGCGGT
TATCTCGCAATCGCTACTCAAACGCCAAACGGGTGGCCGTGTCGCCGCGCATGAAATCATGATTGGCACCCCTGCCATTC
GTAACTTGATTCGTGAAAACAAAGTCGCCCAGATGTACTCTGCCATCCAAACCAGTGCGGGCGATGGTATGACCACACTT
GATCAAAGCCTTCGCGAGCTGGTCAATAAAGGCGTGATTAGCAAAGAATCGGCGCGCCAATATGCCAAACAACCGGAAAG
CTTTATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378Q9P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

80.58

98.854

0.797
  pilT Acinetobacter baumannii D1279779

80.58

98.854

0.797
  pilT Acinetobacter baumannii strain A118

80.58

98.854

0.797
  pilT Pseudomonas aeruginosa PAK

77.326

98.567

0.762
  pilT Acinetobacter baylyi ADP1

76.744

98.567

0.756
  pilT Pseudomonas stutzeri DSM 10701

76.453

98.567

0.754
  pilT Legionella pneumophila strain Lp02

73.837

98.567

0.728
  pilT Legionella pneumophila strain ERS1305867

73.837

98.567

0.728
  pilT Neisseria meningitidis 8013

67.536

98.854

0.668
  pilT Neisseria gonorrhoeae MS11

67.246

98.854

0.665
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.358

95.989

0.656
  pilT Vibrio cholerae strain A1552

68.358

95.989

0.656
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.149

100

0.521
  pilU Acinetobacter baylyi ADP1

40.751

99.14

0.404
  pilU Pseudomonas stutzeri DSM 10701

41.317

95.702

0.395
  pilU Vibrio cholerae strain A1552

40.923

93.123

0.381