Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6G27_RS04920 Genome accession   NZ_CP065751
Coordinates   1102442..1103593 (-) Length   383 a.a.
NCBI ID   WP_062331669.1    Uniprot ID   A0A378QCI1
Organism   Moraxella osloensis strain FDAARGOS_870     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1097442..1108593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G27_RS04910 (I6G27_04910) ribF 1100683..1101708 (-) 1026 WP_062331675.1 riboflavin biosynthesis protein RibF -
  I6G27_RS04915 (I6G27_04915) - 1101834..1102370 (+) 537 WP_062331672.1 GNAT family N-acetyltransferase -
  I6G27_RS04920 (I6G27_04920) pilU 1102442..1103593 (-) 1152 WP_062331669.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6G27_RS04925 (I6G27_04925) pilT 1103683..1104732 (-) 1050 WP_062331666.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G27_RS04930 (I6G27_04930) - 1105005..1105730 (+) 726 WP_062331663.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6G27_RS04935 (I6G27_04935) - 1106156..1106680 (+) 525 WP_062331660.1 C40 family peptidase -
  I6G27_RS04940 (I6G27_04940) - 1107018..1108367 (+) 1350 WP_062331657.1 diguanylate cyclase -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42953.24 Da        Isoelectric Point: 5.9759

>NTDB_id=513969 I6G27_RS04920 WP_062331669.1 1102442..1103593(-) (pilU) [Moraxella osloensis strain FDAARGOS_870]
MDFDKLLQLMVQKNGSDLFITADAPPSMKVNGTIMPVTKSALTPEQSMTLVKSVMTPSQIKEFDETLECQFAISDKHQNA
RFRVSAFVQKDCAGMVLRKIETKIPTVEQLNLPPILNEIAMKKRGIVLVVGATGTGKSTTLAAMMGYRNENSRGHIITIE
DPIEYVHEHKGCIITQREVGIDTKSFEAGLKNTLRQAPDVILIGEIRTRETMDYAIQYAETGHLVFATLHANNANQAIDR
IIHFFDAEHHEQILMDLSLNLRAIVAQQLIATPDGKGRRAAIEILLNSQLIGDYIRKGEIHEIKPVMKRSRELGMQTFDQ
ALFDLYENGEITYRDAIKHADSPNDLRLLIKLESKSANKIDENELNFSLQSDDNSAANENTMF

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=513969 I6G27_RS04920 WP_062331669.1 1102442..1103593(-) (pilU) [Moraxella osloensis strain FDAARGOS_870]
ATGGATTTTGATAAATTACTGCAGCTAATGGTACAAAAAAACGGCTCTGACCTTTTTATCACCGCCGATGCGCCACCATC
AATGAAAGTCAATGGTACTATCATGCCAGTCACCAAGTCAGCGCTGACCCCAGAGCAATCGATGACGCTGGTCAAAAGCG
TGATGACGCCTAGTCAAATCAAAGAATTTGACGAAACCCTCGAATGCCAGTTTGCCATTAGTGACAAACACCAAAACGCC
CGCTTTCGTGTCTCTGCCTTTGTCCAAAAAGATTGTGCGGGCATGGTGCTACGTAAAATTGAAACCAAAATTCCGACGGT
AGAGCAACTCAATCTACCGCCGATTTTGAATGAAATTGCTATGAAAAAACGCGGTATCGTGCTAGTGGTCGGTGCGACGG
GTACAGGTAAATCTACCACGCTGGCAGCTATGATGGGTTATCGCAACGAGAATTCGCGCGGTCATATCATCACCATCGAA
GACCCGATTGAATACGTCCATGAGCATAAAGGCTGTATCATCACCCAACGCGAAGTTGGCATTGACACCAAATCATTTGA
AGCAGGATTAAAAAACACCCTGCGTCAAGCACCTGATGTGATTTTGATTGGCGAGATTCGTACCCGCGAGACCATGGATT
ATGCCATTCAATATGCCGAAACAGGACACTTGGTGTTTGCCACCCTCCACGCTAACAACGCCAACCAAGCGATTGACCGT
ATCATTCACTTTTTTGACGCAGAACATCATGAGCAGATTTTGATGGATTTGTCATTAAACCTTCGCGCCATTGTAGCGCA
GCAATTGATTGCCACCCCTGATGGCAAAGGCCGCCGCGCTGCGATTGAAATCTTGCTCAATTCGCAACTGATTGGCGATT
ACATCCGTAAAGGGGAAATCCACGAAATTAAGCCCGTGATGAAACGCTCTCGTGAACTTGGCATGCAAACCTTTGACCAA
GCGTTGTTTGATTTGTATGAAAACGGCGAAATCACCTACCGCGATGCCATCAAACACGCTGACTCACCCAACGATTTACG
CCTACTTATTAAGCTTGAGAGTAAATCAGCCAATAAAATTGATGAAAATGAACTAAATTTCTCCCTGCAATCTGATGATA
ATAGCGCCGCAAATGAGAACACAATGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378QCI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.258

92.95

0.634
  pilU Acinetobacter baylyi ADP1

65.11

95.039

0.619
  pilU Vibrio cholerae strain A1552

55.271

91.645

0.507
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.02

91.645

0.394
  pilT Acinetobacter baumannii strain A118

42.274

89.556

0.379
  pilT Acinetobacter nosocomialis M2

42.274

89.556

0.379
  pilT Acinetobacter baumannii D1279779

42.274

89.556

0.379
  pilT Legionella pneumophila strain ERS1305867

42.857

87.728

0.376
  pilT Legionella pneumophila strain Lp02

42.857

87.728

0.376
  pilT Acinetobacter baylyi ADP1

41.691

89.556

0.373
  pilT Vibrio cholerae strain A1552

42.296

86.423

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.296

86.423

0.366
  pilT Pseudomonas aeruginosa PAK

40.462

90.339

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.462

90.339

0.366
  pilT Neisseria gonorrhoeae MS11

40.588

88.773

0.36
  pilT Neisseria meningitidis 8013

40.588

88.773

0.36