Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   I6G33_RS13355 Genome accession   NZ_CP065721
Coordinates   2793492..2794526 (-) Length   344 a.a.
NCBI ID   WP_008568507.1    Uniprot ID   A0A8E2QBM5
Organism   Stutzerimonas degradans strain FDAARGOS_876     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2788492..2799526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G33_RS13330 (I6G33_13330) pyrR 2788893..2789405 (+) 513 WP_102829093.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  I6G33_RS13335 (I6G33_13335) - 2789415..2790431 (+) 1017 WP_102829094.1 aspartate carbamoyltransferase catalytic subunit -
  I6G33_RS13340 (I6G33_13340) - 2790431..2791702 (+) 1272 WP_102829095.1 dihydroorotase -
  I6G33_RS13345 (I6G33_13345) - 2791786..2792181 (-) 396 WP_008568505.1 TM2 domain-containing protein -
  I6G33_RS13350 (I6G33_13350) pilU 2792291..2793436 (-) 1146 WP_008568506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6G33_RS13355 (I6G33_13355) pilT 2793492..2794526 (-) 1035 WP_008568507.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G33_RS13360 (I6G33_13360) - 2794593..2795282 (+) 690 WP_102829096.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6G33_RS13365 (I6G33_13365) proC 2795293..2796117 (+) 825 WP_102829097.1 pyrroline-5-carboxylate reductase -
  I6G33_RS13370 (I6G33_13370) - 2796127..2796717 (+) 591 WP_008568510.1 YggT family protein -
  I6G33_RS13375 (I6G33_13375) - 2796834..2798801 (+) 1968 WP_102829098.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37900.65 Da        Isoelectric Point: 6.8359

>NTDB_id=513504 I6G33_RS13355 WP_008568507.1 2793492..2794526(-) (pilT) [Stutzerimonas degradans strain FDAARGOS_876]
MDITELLAFSAKQGASDLHLSSGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVVSQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGALGMQTLDACL
KGLLAKGLISRDSAKEKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=513504 I6G33_RS13355 WP_008568507.1 2793492..2794526(-) (pilT) [Stutzerimonas degradans strain FDAARGOS_876]
ATGGATATCACAGAACTGCTGGCCTTCAGCGCCAAGCAGGGCGCGTCCGACCTGCACCTTTCCTCCGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTGCGGCGGATCAACCTGCCGGCGATGGACCACAAGCAGGTGCACGCGCTTATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAAGAATTCCTCGAGACCGACTTCTCCTTCGAGGTGCCCGGCGTCGCGCGC
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGGACCATTCCCTCCAAGGTGCTGACGATGGA
AGACCTGGGCATGGGCGAGGTCTTCCGCAAGATCACCGACGTACCGCGCGGTCTGGTGCTGGTCACCGGTCCGACCGGCT
CGGGCAAGTCGACCACCCTGGCGGCGATGCTTGATTACCTGAACAACACCAAGTACCACCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTTCACGAATCGAAGAAGTGCCTGGTCAACCAGCGCGAAGTGCACCGCGACACGCTGGGCTTCGCCGA
AGCGCTGCGTTCGGCGCTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTGGTATTCGGCACGCTGCACACCACCTCGGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCAGCCGAGGAAAAGTCGATGGTGCGCTCCATGCTGTCCGAGTCGCTGCAGGCGGTGGTTTCCCAGAC
CCTGCTGAAGAAGGTCGGCGGCGGACGGGTGGCGGCGCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGTGGCGCGCTGGGCATGCAGACCCTCGATGCGTGCCTC
AAGGGCCTGCTCGCCAAGGGGCTGATCAGCCGCGACAGCGCCAAGGAAAAGGCCAAGCAGCCGGAAAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

98.547

100

0.985
  pilT Pseudomonas aeruginosa PAK

93.023

100

0.93
  pilT Acinetobacter baumannii D1279779

84.012

100

0.84
  pilT Acinetobacter baumannii strain A118

84.012

100

0.84
  pilT Acinetobacter nosocomialis M2

83.721

100

0.837
  pilT Acinetobacter baylyi ADP1

83.43

100

0.834
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Neisseria meningitidis 8013

67.826

100

0.68
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

40.413

98.547

0.398
  pilU Acinetobacter baylyi ADP1

38.439

100

0.387