Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I6G33_RS13350 Genome accession   NZ_CP065721
Coordinates   2792291..2793436 (-) Length   381 a.a.
NCBI ID   WP_008568506.1    Uniprot ID   A0A8E2QBM6
Organism   Stutzerimonas degradans strain FDAARGOS_876     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2787291..2798436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G33_RS13320 (I6G33_13320) - 2787876..2788445 (+) 570 WP_043299512.1 YqgE/AlgH family protein -
  I6G33_RS13325 (I6G33_13325) ruvX 2788442..2788870 (+) 429 WP_043299511.1 Holliday junction resolvase RuvX -
  I6G33_RS13330 (I6G33_13330) pyrR 2788893..2789405 (+) 513 WP_102829093.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  I6G33_RS13335 (I6G33_13335) - 2789415..2790431 (+) 1017 WP_102829094.1 aspartate carbamoyltransferase catalytic subunit -
  I6G33_RS13340 (I6G33_13340) - 2790431..2791702 (+) 1272 WP_102829095.1 dihydroorotase -
  I6G33_RS13345 (I6G33_13345) - 2791786..2792181 (-) 396 WP_008568505.1 TM2 domain-containing protein -
  I6G33_RS13350 (I6G33_13350) pilU 2792291..2793436 (-) 1146 WP_008568506.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I6G33_RS13355 (I6G33_13355) pilT 2793492..2794526 (-) 1035 WP_008568507.1 type IV pilus twitching motility protein PilT Machinery gene
  I6G33_RS13360 (I6G33_13360) - 2794593..2795282 (+) 690 WP_102829096.1 YggS family pyridoxal phosphate-dependent enzyme -
  I6G33_RS13365 (I6G33_13365) proC 2795293..2796117 (+) 825 WP_102829097.1 pyrroline-5-carboxylate reductase -
  I6G33_RS13370 (I6G33_13370) - 2796127..2796717 (+) 591 WP_008568510.1 YggT family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42402.62 Da        Isoelectric Point: 6.5059

>NTDB_id=513503 I6G33_RS13350 WP_008568506.1 2792291..2793436(-) (pilU) [Stutzerimonas degradans strain FDAARGOS_876]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKINGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEDLKLPDVLKKLAMTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEFIHQHQSCIVTQREVGIDTESFDVALKNTLRQAPDVILIGEVRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQQQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKNLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALAHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDDDPGRSFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=513503 I6G33_RS13350 WP_008568506.1 2792291..2793436(-) (pilU) [Stutzerimonas degradans strain FDAARGOS_876]
ATGGAATTCGAGAAACTGTTGCGCCTGATGGTGGAAAAAGGCGGCTCCGATCTGTTCATCACGGCCGGTGTACCGCCGTC
GATGAAGATCAACGGCAAGATCATGCCGGTGAGCAAAACGCCGATGTCGCCGGAGATGACCCGCGAAACGGTGCACGGCG
TGATGAACGAACAGCAGCGTCGCGAGTTCACCGAAAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGTATCGGGCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGCCGGCATGGTGCTGCGTCGCATCGAAACCAACATCCCGACCATCGA
AGACCTAAAGCTGCCCGATGTGCTGAAGAAGCTGGCGATGACCAAGCGCGGTCTGGTGCTGTTCGTCGGCGCCACGGGTA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCGAGCGGTCACATCATTTCCATCGAGGAC
CCGATCGAGTTCATCCACCAGCATCAGAGCTGCATCGTCACTCAGCGCGAGGTCGGCATCGACACCGAATCGTTCGATGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGGTGCGGACCCGCGAGACGATGGACTACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGCCGACCGGCAGCAGCAGGTGTGGATGGATCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGACGCCGGATGGCAAGGGTCGCCGTGCGGTCATCGAGGTGCTGATCAATACTCCGCTGGCCGCCGACCTGA
TCCGCAAGGGTGAGGTGCACGAGCTGAAGAATCTGATGAAGCGCTCCACCGAACTGGGCATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTCCAGGGCGAGATCACCTACGAAGACGCGCTGGCTCATGCCGACTCGGCCAACGACCTGCGTCT
GCTGATCAAGCTGGGTTCGGAAACCGACGGCGAGCATCTGACCAGTGTGTCGCAGGGCCTGAGCCTGGAAGTCAGCGACG
ACGATCCGGGGCGTAGCTTCCGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

95.801

100

0.958
  pilU Acinetobacter baylyi ADP1

66.387

93.701

0.622
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.953

88.976

0.391
  pilT Vibrio cholerae strain A1552

41.124

88.714

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.124

88.714

0.365
  pilT Legionella pneumophila strain Lp02

41.194

87.927

0.362
  pilT Legionella pneumophila strain ERS1305867

41.194

87.927

0.362