Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q7A_RS03270 Genome accession   NC_017857
Coordinates   693781..694818 (+) Length   345 a.a.
NCBI ID   WP_014705911.1    Uniprot ID   I1XGL7
Organism   Methylophaga nitratireducenticrescens     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 688781..699818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7A_RS03240 (Q7A_684) pyrE 689131..689772 (-) 642 WP_014705905.1 orotate phosphoribosyltransferase -
  Q7A_RS03245 (Q7A_685) metW 689769..690371 (-) 603 WP_014705906.1 methionine biosynthesis protein MetW -
  Q7A_RS03250 (Q7A_686) - 690371..691519 (-) 1149 WP_014705907.1 homoserine O-acetyltransferase -
  Q7A_RS03255 (Q7A_687) - 691541..692116 (-) 576 WP_014705908.1 YggT family protein -
  Q7A_RS03260 (Q7A_688) proC 692109..692942 (-) 834 WP_014705909.1 pyrroline-5-carboxylate reductase -
  Q7A_RS03265 (Q7A_689) - 692991..693689 (-) 699 WP_014705910.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q7A_RS03270 (Q7A_690) pilT 693781..694818 (+) 1038 WP_014705911.1 type IV pilus twitching motility protein PilT Machinery gene
  Q7A_RS03275 (Q7A_691) pilU 694828..695970 (+) 1143 WP_014705912.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q7A_RS03280 (Q7A_692) - 696143..696994 (+) 852 WP_014705913.1 OmpA family protein -
  Q7A_RS03285 (Q7A_693) - 697396..697587 (+) 192 WP_014705914.1 CsbD family protein -
  Q7A_RS03290 (Q7A_694) - 697803..698363 (+) 561 WP_202971549.1 BON domain-containing protein -
  Q7A_RS03295 (Q7A_695) - 698371..698832 (+) 462 WP_014705916.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38660.27 Da        Isoelectric Point: 6.5792

>NTDB_id=51053 Q7A_RS03270 WP_014705911.1 693781..694818(+) (pilT) [Methylophaga nitratireducenticrescens]
MDITELLTFSAKNNASDLHISSGEPPMIRVDGDIKRINLPAMEHKEVHAMIYDIMNDRQRKDFEEFLEADFSFEIPNLAR
FRVNVFNQNRGAAAVFRTIPSTVLTLEELGAPEIFKQISDNNRGIVLVTGPTGSGKSTTLAAMINYRNEKDNEHILTIED
PIEFVHRSKKCLINQREVHRDTHGFSEALRSALREDPDIVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKDMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMIGTPAIRNLIREDKVPQMYSSIQTGGALGMQTLDQCL
QDLVRRQQITKHEALPRAVNKDLFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=51053 Q7A_RS03270 WP_014705911.1 693781..694818(+) (pilT) [Methylophaga nitratireducenticrescens]
ATGGATATTACTGAATTGCTGACCTTTAGTGCAAAAAACAATGCATCCGATTTGCACATCTCCTCTGGTGAGCCGCCAAT
GATTCGGGTTGATGGCGATATAAAACGCATCAATTTGCCCGCCATGGAGCACAAAGAAGTGCATGCCATGATATATGACA
TCATGAACGACCGTCAGCGTAAGGATTTTGAAGAATTTCTGGAGGCAGACTTTTCGTTTGAGATTCCCAACCTGGCGCGT
TTCCGGGTCAATGTGTTTAATCAGAACCGGGGGGCAGCCGCCGTTTTCCGAACCATTCCTTCAACGGTGCTGACACTGGA
AGAATTGGGTGCCCCCGAAATTTTCAAACAGATTTCCGATAATAATCGCGGTATTGTGCTGGTAACCGGTCCGACTGGTT
CCGGTAAGTCAACCACGTTGGCTGCAATGATCAATTATCGTAATGAAAAAGATAACGAACATATTCTGACCATTGAAGAC
CCGATCGAATTTGTACACCGCAGTAAGAAATGTCTGATCAATCAGCGTGAAGTGCATCGTGATACCCATGGCTTCAGTGA
GGCGTTACGCAGTGCTTTGCGGGAAGATCCTGACATAGTTCTGGTCGGTGAATTACGGGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCGGAAACCGGTCACTTGGTATTTGGTACTCTGCATACCTCCTCAGCGGCAAAAACCATAGACCGTATT
ATCGATGTGTTTCCGGCGGCTGAAAAAGATATGGTACGTTCGATGTTGTCAGAATCTTTACGTGCGGTCATTTCCCAGAC
GCTATTGAAGAAAGTCGGGGGTGGGCGGATTGCGGCACATGAAATCATGATTGGTACGCCGGCTATCCGTAATCTGATTC
GTGAAGATAAAGTCCCGCAAATGTATTCTTCAATTCAAACAGGTGGTGCCTTAGGAATGCAGACATTGGATCAGTGTTTA
CAGGATTTGGTACGTCGTCAGCAAATCACCAAGCATGAAGCATTACCCCGTGCCGTGAACAAGGATCTGTTCCGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I1XGL7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

79.586

97.971

0.78
  pilT Acinetobacter baylyi ADP1

78.299

98.841

0.774
  pilT Acinetobacter baumannii D1279779

77.326

99.71

0.771
  pilT Acinetobacter baumannii strain A118

77.326

99.71

0.771
  pilT Acinetobacter nosocomialis M2

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Neisseria meningitidis 8013

69.388

99.42

0.69
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.171

96.522

0.687
  pilT Vibrio cholerae strain A1552

71.171

96.522

0.687
  pilT Neisseria gonorrhoeae MS11

69.096

99.42

0.687
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Vibrio cholerae strain A1552

40.413

98.261

0.397

Multiple sequence alignment